Indica rice target line for gene stacking of specific sites mediated by recombinase

A technology of recombination sites and recombinases, applied in the field of indica rice target lines, can solve problems such as genetic transformation difficulties, achieve the effects of reducing separation sites, reducing breeding workload, and reducing release evaluation costs
CN111118058AActive Publication Date: 2020-05-08SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
Publication Date
2020-05-08

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Abstract

The present invention discloses five starting target lines of gene stacking of specific sites mediated by recombinase suitable for indica rice. Each starting target line has an accurate single copy transgenic DNA, the DNA does not affect endogenous genes, an insertion site is at least 1 kb away from a nearest gene coding region, the DNA is far away from centromere, and a reporter gene gfp is wellexpressed. The target line contains attP and Lox recombination sites, which is conducive to stacking relevant functional genes on the gene sites through site-specific recombination fixed-point stacking. Therefore, the number of isolation sites can be reduced, and breeding workload of transgenic infiltration from experimental strains into local fine varieties can be greatly reduced. In addition, commercial product developers can also integrate the existing transgenic sites, thereby greatly reducing cost of release evaluation.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to an indica rice target line for gene stacking of specific sites mediated by recombinases. Background technique

[0002] Transgenic technology is the use of traditional breeding methods to introduce commercial varieties. However, hybridization between different varieties can lead to heterozygosity of many important agronomic trait genes. For breeding, a breeding line must be homozygous for traits, not only transgenic traits, but also all other good traits related to field elite varieties. For diploid and diploid-like allopolyploid plants, the proportion of lines homozygous for n independent traits by segregation in the absence of genetic linkage is (1 / 4) n . For example, the probability of obtaining homozygous lines with 6 excellent traits and 1 transgenic trait is (1 / 4) 7 , but if 3 more transgenic loci are added, then the proportion of homozygous lines obtained is (1 / ...

Claims

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