Breeding method of semiprostrate high-yield peanut variety

A technology for peanuts and varieties, applied in the agricultural field, can solve the problems of weak branches and unfavorable mechanized excavation, and achieve the effects of speeding up the breeding process, saving the breeding workload, and shortening the generation interval.

Active Publication Date: 2017-08-22
CROP RES INST GUANGDONG ACAD OF AGRI SCI
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  • Description
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  • Application Information

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Problems solved by technology

Due to the upright peanut plant type, the clusters are compact and

Method used

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  • Breeding method of semiprostrate high-yield peanut variety
  • Breeding method of semiprostrate high-yield peanut variety
  • Breeding method of semiprostrate high-yield peanut variety

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1. Directed screening of hybrid parents using three SSR molecular marker primer sets related to yield traits

[0031] 1. According to the survey results of peanut traits (Table 1), randomly select 9 local varieties of semi-climbing peanuts (Wuchuan Mengdou, Yongfa Douyi, Gaohe No. , Huilai Axe, Shengtengxi, Zhuyouzai), 9 erect flower varieties (Yueyou No. 5, Zhongkaihua No. 1, Shanyou 851, Zhanyou 75, Heyou No. 9, Yueyou 52, Yueyou 13, Yueyou 79, Hanghua 2), provided by the Crop Research Institute of Guangdong Academy of Agricultural Sciences.

[0032] 2. The peanuts of the above 16 varieties are germinated, and when the sprouts grow about 1 cm, cut out the sprouts and extract DNA. According to the operating instructions of the Plant Genomic DNA Extraction Kit (spin column type) of Beijing Biotek Biotechnology Co., Ltd., the samples were detected by 1% agarose gel electrophoresis, and the samples were diluted to 25ngμL-1 and stored at -20°C for later use.

[0033] 3. ...

Embodiment 2

[0054] 1. Among the semi-vine varieties, choose the variety Katsudosei with a statistical result of 0-0-0 as the hybrid female parent, and the traits of the female parent are semi-vine peanuts. Among the erect varieties, the variety Hanghua No. 2 with a statistical result of 1-1-1 was selected as the hybrid male parent. F1 generation seeds were obtained by crossing.

[0055] 2. The spare seeds of the F1 generation are sown in a single seed, self-crossed, and harvested in a single plant. Get the F2 generation seeds

[0056] 3. Sow single seed of the F2 generation spare seeds, self-cross, after the peanut leaves grow, take the young peanut leaves, extract DNA, amplify with markers GM1445, GM12531, GM2589, and select the statistical result as 1 according to the band pattern results -1-1 peanut plants, select semi-vine type plants from the above-mentioned peanut plants during the harvest period, and harvest them to obtain F3 generation seeds. The semi-vine type plant needs to h...

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Abstract

The invention provides a breeding method of a semiprostrate high-yield peanut variety. The breeding method is a method of screening male parent and female parent peanut varieties for hybridization by means of three yield trait related SSR marked primer units: GM1445, GM2531 and GM2589. The breeding method provided by the invention is a novel variety breeding method capable of predicting the yield condition of single strains on the premise of no need to wait for harvesting and polymerizing semiprostrate and high-yield trait to a same peanut plant. The method has the advantages of being simple in breeding method, clear in breeding target, small in blindness, high in efficiency and etc., and the output result can be suitable for the general orientation of agricultural mechanization in the future.

Description

technical field [0001] The invention relates to the technical field of agriculture, in particular to a breeding method for a semi-vine type high-yield peanut variety. Background technique [0002] Peanut is one of the four major oil crops in the world, and it is also an important oil crop and economic crop in my country. For a long time, peanut breeding has been mainly based on traditional hybrid breeding, which has a long breeding cycle and a single genetic basis for the bred varieties. With the development of genome sequencing technology and molecular quantitative genetics, a peanut genetic map based on SSR markers has been constructed, and genes / QTLs related to disease resistance, stress resistance, yield, quality, etc. have been located, and molecular markers have also been proposed. Assisted selection breeding techniques. However, the target traits of peanut genetic improvement, especially the yield traits, are mostly quantitative traits, which are controlled by micro...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04C12Q1/68
CPCA01H1/02A01H1/04C12Q1/6895C12Q2600/13C12Q2600/156
Inventor 李杏瑜洪彦彬温世杰梁炫强陈小平
Owner CROP RES INST GUANGDONG ACAD OF AGRI SCI
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