The invention relates to a multifunctional fully-automatic Gram staining instrument. The instrument comprises a glass slide lateral movement device, a microbe automatic staining device, a glass slide entering and departing device, a push rod device and a liquid path control device, the push rod device pushes a coated glass slide positioned on the glass slide entering and departing device into the glass slide lateral movement device, the glass slide moves with a first glass slide rack on a reciprocating movement mechanism to the automatic staining device, is stained, cleaned and blow-dried, the liquid path control device cooperates a computer program in order to control a staining solution in a Gram staining solution bottle to carry out staining, water spraying, gas spraying, drying and other fully-automatic activities on the coated glass slide according to preset steps and time, and the push rod device moves the processed glass slide into the glass slide entering and departing device in order to carry out subsequent operation. The instrument has the advantages of fully-automatic realization of the whole Gram staining process, accurate delivery, cross infection avoiding, staining solution waste avoiding and manpower resource saving.