The invention discloses a greenhouse three-layer wireless network measurement and control system and a layering and clustering method thereof, which comprehensively use wireless sensor network technology, wireless mobile network technology and the like. In the system and the method, a three-layer wireless network consists of a high-layer base station node, a greenhouse group control terminal, an intermediate-layer convergence cluster head node and a lower-layer nodes wireless sensor network node, the intermediate-layer convergence cluster head node is configured according to node cost, operation cost and the requirement on convenience information processing, and the configuration optimization of a lower-layer convergence cluster head node is realized by improving the selection method of aclassic LEACH clustering algorithm the cluster head nodes and applying a multi-objective optimization method. The method is favorable for reducing information transmission delay, cost and power consumption and improving information transmission efficiency. The system can be widely applied to agricultural facilities such as greenhouses, plastic tents and the like, and other indoor environments suchas hospital buildings, hotels or hotel buildings, halls, studio halls, superstores, supermarkets and the like to realize information wireless measurement and control.