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Application of Ghd7 gene in regulating rice flag leaf chlorophyll content

A technology of chlorophyll content and rice flag leaf, applied in the fields of application, genetic engineering, plant gene improvement, etc., can solve the problem of unreported control of natural genetic variation of chlorophyll, and achieve the effect of reducing chlorophyll content

Inactive Publication Date: 2016-11-23
HUAZHONG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] A series of genes involved in chlorophyll metabolism have been identified using chlorophyll synthesis-deficient mutants or non-functional green-sustaining mutants in rice, such as OsChlH (Jung et al., 2003), OsChlD, OsChlI (Zhang et al., 2006), YGL1 (Wu et al., 2007), NON-YELLOWCOLORING1 (NYC1), NYC1-LIKE (NOL) (Kusaba et al., 2007; Sato et al., 2009), STAYGREEN (SGR) (Jianget al., 2007) and NON-YELLOW COLORING4 (NYC4) (Yamatani et al., 2013), but these genes have not yet reported natural genetic variation controlling chlorophyll content

Method used

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  • Application of Ghd7 gene in regulating rice flag leaf chlorophyll content
  • Application of Ghd7 gene in regulating rice flag leaf chlorophyll content
  • Application of Ghd7 gene in regulating rice flag leaf chlorophyll content

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Genome-wide association analysis detected extremely significant effect sites controlling chlorophyll content in the Ghd7 gene segment

[0031] Taking 533 sequenced core germplasms from the State Key Laboratory of Crop Genetic Improvement of Huazhong Agricultural University as an associated group, a systematic survey was conducted on the chlorophyll content of flag leaves in 2012 and 2013, using linear mixed models (LMM) and multi-locus mixed models ( MLMM) genome-wide association analysis (GWAS) of chlorophyll content-related traits in the entire associated population and each subgroup found that there was a significant hotspot area in the 9.13-9.17Mb interval of chromosome 7, and 9 genes from different GWAS could be repeatedly detected. The extremely prominent signal peak analyzed corresponds to the position of the Ghd7 gene.

[0032] The inspection method of the chlorophyll content of the core germplasm: about 3 days after the earing of the rice, take the h...

Embodiment 2

[0034] Example 2: Relationship between Ghd7 expression level and chlorophyll content in core germplasm materials

[0035] 101 materials were randomly selected from the core germplasm association population, and the relationship between gene expression and chlorophyll content was detected. It was found that the expression of Ghd7 was extremely significantly negatively correlated with 12chla, 12chlb and 12total_chl (Table 2).

[0036] Table 2 The relationship between the expression level of Ghd7 and the traits related to chlorophyll content

[0037]

Embodiment 3

[0038] Example 3: The use of near-isogenic lines and transgenic materials to confirm the regulatory effect of Ghd7 on the content of chlorophyll in flag leaves

[0039] The materials NIL (mh7) and NIL (zs7) used in the present invention are a pair of near isogenic lines with Zhenshan 97 as the genetic background constructed by the State Key Laboratory of Crop Genetic Improvement of Huazhong Agricultural University (the original near isogenic line Named as the inbred line RIL50, see: the second line in the paragraph "Specific Embodiments" on page 3 of the patent specification of Publication No. CN101148674, the original source of this material can be found in: Xing Yongzhong et al., Mapping and Separation of Rice Plant Height and Heading Date Genes , Acta Botanica, 2001, 43(7):721-726 Weiya), using the near-isogenic line, Dr. Xue Weiya carried out map-based cloning of the Ghd7 gene (Weiya Xue., et al.Natural variation in Ghd7 is an important regulator ofheading date and yield p...

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Abstract

The invention belongs to the technical field of plant genetic engineering. It is found that a significant hot spot region exists in the range of 9.13 Mb to 9.17 Mb of a chromosome 7 through genome-wide association study (GWAS), nine signal peak values which have an extremely significant effect on the rice flag leaf chlorophyll content from different GWAS can be detected repeatedly, and the peak values correspond to Ghd7 gene positions. It is verified through near-isogenic lines and genetically modified materials that Ghd 7 serves as a main natural variation site to regulate the rice flag leaf chlorophyll content by down-regulating related gene expressions such as chlorophyll metabolism and chloroplast synthesis. The Ghd 7 has important breeding value in optimizing the rice flag leaf chlorophyll content.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering. It specifically relates to the application of a Ghd7 gene in regulating the chlorophyll content of rice flag leaves. Background technique [0002] Chlorophyll is the main pigment for photosynthesis in higher plants, including chlorophyll a and chlorophyll b. The antenna pigments have the function of collecting and transmitting light energy. The special chlorophyll a molecule in the reaction center can also convert light energy into chemical energy. Chlorophyll is located on the thylakoid membrane and binds to specific proteins through non-covalent bonds to form a pigment-protein complex to perform its function. Chlorophyll b also has the function of stabilizing the light-harvesting pigment-protein complex. [0003] Rice is one of the most important food crops. The chlorophyll content of flag leaf is closely related to the photosynthesis and yield potential of leaves, and is a v...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29A01H5/00
Inventor 王功伟汪全秀
Owner HUAZHONG AGRI UNIV
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