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Rice stress tolerance and rice chlorophyll content related protein, coding gene and application thereof

A technology of chlorophyll content and plant stress tolerance, applied in the fields of application, genetic engineering, plant gene improvement, etc., can solve problems such as unknown protein location, and achieve the effect of enhanced sensitivity

Active Publication Date: 2014-10-15
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most members of this family are localized in chloroplast or mitochondria, but the localization of a few proteins is unknown

Method used

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  • Rice stress tolerance and rice chlorophyll content related protein, coding gene and application thereof
  • Rice stress tolerance and rice chlorophyll content related protein, coding gene and application thereof
  • Rice stress tolerance and rice chlorophyll content related protein, coding gene and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0038] Example 1, the acquisition and identification of WSL gene suppressed expression plants

[0039] 1. Construction of WSL gene RNA interference vector (recombinant plasmid pCUbi1390-△FAD2-WSL)

[0040] 1. Obtaining the interference fragment of WSL gene

[0041] (1) Using the RNAprep pure plant total RNA extraction kit (purchased from Tiangen Biochemical Technology (Beijing) Co., Ltd.), the total RNA of 14-day seedlings of rice Asominori (Oryza sativa) was extracted, and cDNA was obtained by reverse transcription.

[0042] (2) Using the cDNA obtained in step (1) as a template, perform PCR amplification with a primer pair composed of WSL-sense-F and WSL-sense-R to obtain a PCR amplification product.

[0043] WSL-sense-F:5'-TTCTGCACTAGGTACCAGGCCTG ATGGAGGCCGCCGCGTCGCTG-3';

[0044] WSL-sense-R:5'-CTGACGTAGGGGCGATA GAGCTC GTGTAACCCTGCTTCAACTTCATGC-3'.

[0045] (3) Using the cDNA obtained in step (1) as a template, perform PCR amplification with a primer pair composed of ...

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Abstract

The invention discloses a rice stress tolerance and rice chlorophyll content related protein and a coding gene and application thereof. The rice stress tolerance and rice chlorophyll content related protein is named as WSL protein come from rice variety Asominori, and is as follows (a) or (b) or (c): (a) a protein comprising an amino acid sequence shown as sequence 1 in the sequence table; (b) a plant stress tolerance related protein which is derived from the sequence 1, and is formed by substitution and / or deletion and / or addition of one or a plurality of amino acid residues of the amino acid sequence shown as the sequence 1 in the sequence table; and (c) a plant chlorophyll content related protein which is derived from the sequence 1, and is formed by substitution and / or deletion and / or addition of one or a plurality of amino acid residues of the amino acid sequence shown as the sequence 1 in the sequence table. The rice stress tolerance and rice chlorophyll content related protein has important theoretical significance and practical significance on further clarification of the molecular mechanism of plant resistance and cultivating of high quality stress tolerant new crop varieties by the genetic engineering technology and means.

Description

technical field [0001] The invention relates to a protein related to rice stress tolerance and rice chlorophyll content, its coding gene and application. Background technique [0002] Rice is one of the most important food crops, which is the staple food for more than half of the world's population. The rice leaf color mutant is an ideal material for studying the processes of plant chloroplast development, photomorphogenesis and photosynthesis. On the basis of mutants, revealing the molecular mechanism of chloroplast development and morphogenesis is the premise of improving rice light energy use efficiency and increasing yield. In addition, in addition to photosynthesis, chloroplast also communicates complex signals with the nucleus and mitochondria, so as to cooperate to maintain the normal growth and development of plants. [0003] PPR protein is one of the largest protein families in a class of plants, with 480 members in rice species, and half of these proteins contain...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82C12N5/10C12N1/21A01H5/00
CPCC07K14/415C12N15/8269C12N15/8271
Inventor 万建民吴赴清谭俊杰谭振华林启冰王洁王久林程治军郭秀平张欣
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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