5 results about "Chlorophyll synthesis" patented technology

This invention belongs to the field of functional food processing technology, specifically relating to a method for improving the flavor of a chlorophyll-deficient Chlorella proteoglycan mutant. The method includes the following steps: S1, obtaining a Chlorella proteoglycan mutant strain; S2, centrifuging the Chlorella proteoglycan mutant strain and collecting the algal sludge, then pulverizing the collected algal sludge and dispersing it in water to obtain an algal powder suspension, then adding a compound enzyme to the algal powder suspension, and inactivating the enzyme after the reaction; S3, cooling the system to room temperature, adding glucose and stirring to dissolve, sterilizing, inoculating with a compound bacterial strain and fermenting, and sterilizing again after fermentation; S4, cooling the fermented system to room temperature, then adding β-cyclodextrin and stirring, and drying the system after stirring to obtain the algal protein product. This invention employs a synergistic process of compound enzymatic hydrolysis, compound bacterial fermentation, and β-cyclodextrin encapsulation to remove the off-flavor of the mutant algae from the source, significantly improving the flavor and enhancing the product's storage stability.

The application discloses a single-cell green alga chlorophyll synthesis-defective mutant strain and application thereof. The mutant strain includes Parachlorella kessleri SCUT-ACB 4 and SCUT-ACB 4-65, and the preservation numbers are CCTCC NO: M 2022518 and CCTCC NO: M 2022519, which are preserved in the China Center for Type Culture Collection of Wuhan University in Wuhan, China on April 28, 2022. Compared with wild-type algae strains, the mutant strain in the application fundamentally cuts off non-light-dependent chlorophyll synthesis, and the content of chlorophyll a is reduced by more than 98%, and the content of chlorophyll b is reduced by 100%, and the algal body is golden yellow, and the mutant strain can be used for producing protein, and provides high-yield and high-quality fermentation algae for replacing protein in precise fermentation production.

This invention discloses SlCOP1 The application of genes in regulating tomato chlorophyll synthesis and photosynthetic efficiency, through knockout SlCOP1 The gene enables increased chlorophyll content and enhanced photosynthetic efficiency in tomatoes. SlCOP1 Genes affect chlorophyll accumulation by regulating the gibberellin signaling pathway; the tomato SlCOP1 The gene has the nucleotide sequence of SEQ ID No: 1. This invention is the first to elucidate... SlCOP1 A novel gene regulation function for tomato chlorophyll synthesis reveals the core role of the SlCOP1-SlDELLA-GA pathway in chlorophyll synthesis regulation, providing new theoretical basis for elucidating the molecular regulatory network of plant chlorophyll synthesis. SlCOP1 Genes can serve as key target genes for precision breeding, enabling targeted improvement of tomato chlorophyll accumulation and photosynthetic efficiency traits. This provides an efficient and feasible technical path for cultivating new tomato varieties with high light efficiency and high yield, and has significant theoretical implications and broad prospects for agricultural applications.

The application discloses a gallate-based blue light-emitting material applicable to a plant light supplement lamp, a preparation method and application of the gallate-based blue light-emitting material, and a chemical general formula of the light-emitting material is Ca 1‑x Ga4O7:xCd 2+ , wherein x is 0.003-0.007. The gallate-based blue light-emitting material takes CaGa4O7 as a matrix, Cd 2+ is a doping ion, Cd 2+ cannot form a new light-emitting center in CaGa4O7, and only plays a role in enhancing blue light emission of the matrix. The light-emitting material produces a wide peak with a peak value of 445 nm under ultraviolet excitation, generates blue-white emission, and the emission band is highly coincident with a band required by plant growth, so that chlorophyll synthesis can be promoted, stem and leaf growth of plants is beneficial, the plant light supplement demand can be met, and the optimal afterglow duration can reach nearly 2 hours. The light supplement can still be carried out after stopping excitation, and energy consumption is reduced.

The application discloses OsRNE A gene and application of the gene and a coded protein in rice leaf color regulation and chloroplast development. OsRNE The nucleotide sequence of the gene is shown as SEQ ID NO. 1, and the amino acid sequence of the coded protein is shown as SEQ ID NO. 2. OsRNE The application utilizes CRISPR / Cas9 gene editing technology to knockout the gene and obtain a loss-of-function mutant. The mutation of the gene leads to impaired chloroplast development of rice, affects chlorophyll synthesis, albino of seedlings and death within three-leaf stage, and thus can be used as a marker trait to assist rice molecular breeding at the seedling stage. OsRNE The gene is mainly expressed in leaves, the coded protein is located in chloroplasts, and the chloroplast development and normal growth of seedlings are affected by affecting the metabolism of ribosomal RNA in chloroplasts. Therefore, the application can be applied to molecular genetic breeding of rice leaf color traits, and has important significance for further understanding of a chloroplast development regulation mechanism of rice and yield increase of rice.