The invention provides a method for improving
selenium tolerance of
euglena gracilis and a preparation method of
selenium-rich microalgae, and belongs to the technical field of microalgae application. The invention provides a method for improving
selenium tolerance of
euglena gracilis. The method comprises the following steps: culturing
euglena gracilis in a culture medium added with
sodium selenite; the adding mode of the
sodium selenite comprises the step of adding the
sodium selenite once a day from the beginning of inoculating the
euglena gracilis; or sodium selenite is added once after the
euglena gracilis is cultured for 48 hours or two days; or, starting from
inoculation of the
euglena gracilis, after the euglena gracilis is cultured for 48 hours or 2 days, sodium selenite is added once a day; the addition amount of the sodium selenite in each time is 1-10 mg / L. The method can prolong the
growth cycle of the euglena gracilis, improve the movement ability of the euglena gracilis, promote the accumulation of parastarch, improve the maximum photosynthetic rate of the euglena gracilis, promote the
chlorophyll synthesis of the euglena gracilis and improve the
catalase activity of the euglena gracilis, and is beneficial to improving the selenium tolerance of the euglena gracilis.