Stenotrophomonas sp. strain KT48, algicidal bacterial liquid as well as preparation method and application of algicidal bacterial liquid
A single-cell, algicidal technology, applied in the biological field, can solve the problems of endogenous pollution of algae, algal blooms have not been effectively controlled for a long time, secondary pollution, etc., to reduce chlorophyll content, environmental friendliness and bio- Degradation, no secondary pollution effect
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[0042] The present disclosure also provides a method for preparing algae solution, which may include the following steps:
[0043] S1. Take the cryopreserved Oligoxomonas strain KT48 with the preservation number CGMCC NO.22722, and inoculate it in the first culture medium after high-temperature sterilization;
[0044] S2. Place the inoculated first culture medium in a constant temperature oscillating incubator with a temperature of 25 to 40°C and a rotation speed of 130 to 230rmp for 24 to 72 hours to obtain an activated bacterial strain; wherein, the temperature of the constant temperature oscillating incubator can be 25 ℃, 30°C, 35°C, 37°C or 40°C, etc., there is no specific limit; the rotation speed of the constant temperature shaking incubator can be 130rmo, 150rmp, 170rmp, 180rmp, 200rmp, 215rmp or 230rmp, etc., there is no specific limit; the incubation time It can be 24h, 30h, 36h, 42h, 48h, 55h, 60h, 66h, 70h or 72h, etc., without limitation;
[0045]S3, according to ...
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[0058] Example. Isolation and identification of oligooxymonas bacterial strain KT48 (Stenotrophomonas sp.KT48)
[0059] 1. Isolation and screening of strain KT48
[0060] (1) Cyanobacteria blooms in pit ponds in the upstream waters of Yankou Reservoir in Yiwu City, Zhejiang Province often break out. Collect water samples after the blooms in this water area, and first use a 1.2 μm filter membrane to filter the membrane to remove large particles and impurities in the water; Then use a 0.45 μm filter membrane to filter the membrane, cut the 0.45 μm filter membrane into pieces, put it into the high-pressure sterilized beef extract peptone liquid medium, and place it in a 180rmp constant temperature shaking incubator for 36 hours;
[0061] (2) Dilute the sample obtained in step (1) by 10 4 times, spread on a nutrient agar solid plate, and place in a 35°C incubator for 36 hours;
[0062] (3) Pick different types of single colony, streak on the nutrient agar solid plate, and place ...
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