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Stenotrophomonas sp. strain KT48, algicidal bacterial liquid as well as preparation method and application of algicidal bacterial liquid

A single-cell, algicidal technology, applied in the biological field, can solve the problems of endogenous pollution of algae, algal blooms have not been effectively controlled for a long time, secondary pollution, etc., to reduce chlorophyll content, environmental friendliness and bio- Degradation, no secondary pollution effect

Pending Publication Date: 2021-12-10
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But in fact, the endogenous pollution of water bodies has not been reduced, and algae blooms have not been effectively controlled for a long time, but have fallen into a repeated cycle of "outbreak-treatment-outbreak"
The use of clay to induce bloom flocculation is the only effective algae control measure at present, but the algae that settle into the water body may be converted into endogenous pollution, and there is a risk of secondary pollution

Method used

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  • Stenotrophomonas sp. strain KT48, algicidal bacterial liquid as well as preparation method and application of algicidal bacterial liquid
  • Stenotrophomonas sp. strain KT48, algicidal bacterial liquid as well as preparation method and application of algicidal bacterial liquid
  • Stenotrophomonas sp. strain KT48, algicidal bacterial liquid as well as preparation method and application of algicidal bacterial liquid

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Experimental program
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preparation example Construction

[0042] The present disclosure also provides a method for preparing algae solution, which may include the following steps:

[0043] S1. Take the cryopreserved Oligoxomonas strain KT48 with the preservation number CGMCC NO.22722, and inoculate it in the first culture medium after high-temperature sterilization;

[0044] S2. Place the inoculated first culture medium in a constant temperature oscillating incubator with a temperature of 25 to 40°C and a rotation speed of 130 to 230rmp for 24 to 72 hours to obtain an activated bacterial strain; wherein, the temperature of the constant temperature oscillating incubator can be 25 ℃, 30°C, 35°C, 37°C or 40°C, etc., there is no specific limit; the rotation speed of the constant temperature shaking incubator can be 130rmo, 150rmp, 170rmp, 180rmp, 200rmp, 215rmp or 230rmp, etc., there is no specific limit; the incubation time It can be 24h, 30h, 36h, 42h, 48h, 55h, 60h, 66h, 70h or 72h, etc., without limitation;

[0045]S3, according to ...

Embodiment

[0058] Example. Isolation and identification of oligooxymonas bacterial strain KT48 (Stenotrophomonas sp.KT48)

[0059] 1. Isolation and screening of strain KT48

[0060] (1) Cyanobacteria blooms in pit ponds in the upstream waters of Yankou Reservoir in Yiwu City, Zhejiang Province often break out. Collect water samples after the blooms in this water area, and first use a 1.2 μm filter membrane to filter the membrane to remove large particles and impurities in the water; Then use a 0.45 μm filter membrane to filter the membrane, cut the 0.45 μm filter membrane into pieces, put it into the high-pressure sterilized beef extract peptone liquid medium, and place it in a 180rmp constant temperature shaking incubator for 36 hours;

[0061] (2) Dilute the sample obtained in step (1) by 10 4 times, spread on a nutrient agar solid plate, and place in a 35°C incubator for 36 hours;

[0062] (3) Pick different types of single colony, streak on the nutrient agar solid plate, and place ...

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Abstract

The invention provides a Stenotrophomonas sp. strain KT48, algicidal bacterial liquid as well as a preparation method and application of the algicidal bacterial liquid. The preservation number of the Stenotrophomonas sp. strain KT48 is CGMCC NO. 22722, and the algicidal bacterial liquid is prepared from the Stenotrophomonas sp. strain KT48 with the preservation number of CGMCC NO. 22722; the Stenotrophomonas sp. strain KT48 has an algae removal effect instead of a metabolite of the Stenotrophomonas sp. strain KT48, so that the Stenotrophomonas sp. strain KT48 is more convenient for practical application; when the algicidal bacterial liquid is prepared, a culture medium is inoculated with the Stenotrophomonas sp. strain KT48, then, the culture medium is cultured in a constant-temperature oscillation box to a stable phase so as to obtain the algicidal bacterial liquid, and thus, the preparation method is simple; the Stenotrophomonas sp. strain KT48 can be used for breaking cell membranes of microcystis aeruginosa and destroying cell structures of the microcystis aeruginosa; and the algicidal bacterial liquid prepared by adopting the Stenotrophomonas sp. strain KT48 can be used for lowering the chlorophyll content of blue-green algae and effectively controlling water bloom outbreak, and has high efficiency, biological specificity, environmental friendliness and biodegradability. When the algicidal bacterial liquid is used for controlling blue-green algae bloom, the cost is low, the operation is simple, the safety is high, and secondary pollution is avoided.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to oligooxymonas bacterial strain KT48, algae-lysing bacteria liquid and its preparation method and application. Background technique [0002] In recent years, the eutrophication of water bodies has attracted widespread attention worldwide, and the frequency of algal blooms has also increased significantly, especially in shallow lakes. Short-term control of algal blooms in freshwater systems is most common using physical and chemical methods. But in fact, the endogenous pollution of water bodies has not been reduced, and algae blooms have not been effectively controlled for a long time, but have fallen into a repeated cycle of "outbreak-treatment-outbreak". The use of clay to induce bloom flocculation is the only effective algae control measure at present, but the algae that settle into the water body may be converted into endogenous pollution, and there is a risk of secondary pollutio...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/20A01P13/02C12R1/01
CPCC12N1/20A01N63/20
Inventor 庄绪亮吕萍徐圣君郑效旭王聪白志辉
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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