Molecular marker influencing oblique length of chicken body and application thereof

A molecular marker and body oblique length technology, which is applied to the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc. Efficiency, the effect of reducing breeding costs

Active Publication Date: 2020-11-13
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Facing the huge demand for chicken and the increasing status quo, although China’s domestic chicken enterprises have met some of the domestic demand for high-quality chicken, there is still a huge gap in the market a

Method used

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  • Molecular marker influencing oblique length of chicken body and application thereof
  • Molecular marker influencing oblique length of chicken body and application thereof
  • Molecular marker influencing oblique length of chicken body and application thereof

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0031] Experimental example 1 Experimental chicken group feeding and body slant length measurement

[0032] (1) Experimental animals

[0033] The test chicken group used in the present invention is the F2 generation resource group obtained by crossing Daweishan miniature chicken and recessive White Rock broiler chicken.

[0034] In the F0 generation, breeders were selected from Daweishan miniature chickens (from the practice chicken farm of Yunnan Agricultural University) and recessive white lock broilers (from Kunming Yunling Guangda Breeder Feed Co., Ltd.), and the selected male and female chickens had this breed Features, high egg production, medium weight, pure blood. Daweishan miniature chicken is a small chicken breed that has not been selected for breeding. It has the advantages of small size (average weight 0.7-1.2kg), less feed consumption, and low basal metabolism. It is a new breed of high-quality and grain-saving chickens (line ) of excellent material. Recessive...

experiment example 2

[0040] Experimental Example 2: Acquisition of Molecular Markers Affecting Chicken Body Slant Length

[0041] (1) Blood sample collection

[0042] Vacuum blood collection tubes containing EDTA-dipotassium were used to collect blood from chicken wing veins.

[0043] (2) Extraction and identification of genomic DNA

[0044] Gentra Puregene Blood Kit (Qiagen) column centrifugation kit was used to extract blood genomic DNA. After the DNA was completely dissolved, its concentration was detected with a NannoDrop nucleic acid analyzer, and the integrity of the DNA band was detected under 1% agarose gel electrophoresis conditions. sex.

[0045] (3) DNA library construction and sequencing

[0046]The construction of the library was completed using the NEBNext DNA library kit (NewEngland Biolabs). The specific operation steps were as follows: firstly, the genomic DNA was randomly broken into small fragments, and then Illumina paired-end adapters were connected to the fragments with a ...

experiment example 3

[0063] Experimental example 3 A kind of SNP molecular marker genotyping detection method that affects chicken body slant length

[0064] (1) Experimental animals

[0065] The test flock group used in the present invention is 300 (150 male and female) of 150-day-old Daweishan miniature chicken breeders of Yunnan Agricultural University, which is the core group of breeder chickens. Measure body oblique length.

[0066] (2) Target DNA sequence amplification and sequencing

[0067] 1) Primer design

[0068] The DNA sequence of chicken chromosome 5 was downloaded from the NCBI website, and primers were designed using the primer design software primer premier 6.0.

[0069] The DNA sequences of the designed primers are as follows:

[0070] Upstream primer primer-F: 5'-GAAGGTCCTCAGGGTACGTC-3';

[0071] Downstream primer primer-R: 5'-AGCCTCACGCAACAGATGAA-3'.

[0072] 2) PCR amplification

[0073] Add 1.0μL DNA template, 10.5uL double distilled water, 2×TSINGKE to the 10uL reacti...

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Abstract

The invention discloses a molecular marker influencing the oblique length of a chicken body and application of the molecular marker, and belongs to the technical field of molecular marker assisted selection and animal genetic breeding. The molecular marker disclosed by the invention is obtained by carrying out genome wide association study (GWAS) analysis on 741 test chickens in F2-generation resource groups established by Dawei Mountain miniature chickens and WRR broilers; a nucleotide single-base mutation (named as Chr.16402009T>C) of T>C exists at the 16402009bp position of a chromosome V of a chicken reference genome Gallus_gallus.GRCg6a version. A gene positioned near the nucleotide single-base mutation is a lysine methyltransferase 5B (KMT5B) gene, and the oblique body length of thechicken is obviously influenced by mutation. The invention discloses acquisition and application of the molecular marker. The invention further provides an SNP molecular marker genotyping detection method influencing the oblique length of the chicken body, an efficient and accurate molecular marker assisted breeding technology can be established by utilizing the method, and the molecular marker assisted breeding technology is applied to chicken growth and development genetic improvement, so that the growth and development of the chicken are improved.

Description

technical field [0001] The invention relates to the field of molecular marker-assisted selection technology and animal genetic breeding technology, in particular to a molecular marker that affects the oblique length of chicken body and its application. Background technique [0002] Chicken is the number one meat product in the world, accounting for 39% of all meat. Chicken is the second largest meat product in China, accounting for 15.2% of the total meat production. As the global population continues to grow, the area of ​​arable land is gradually decreasing. Chicken and eggs are the two types of animal protein with the highest grams of animal protein that can be purchased per unit of currency except milk. They are highly competitive in terms of production costs and will become global meat. The fastest growing category of production. my country is the second largest producer and consumer of broiler chickens in the world. Especially in the case of the shortage of pork caus...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/124C12Q2600/156C12Q2600/172C12Q2531/113Y02P60/87
Inventor 葛长荣豆腾飞贾俊静黄英闫世雄孙帅王坤曹玮娜徐志强谷大海荣华段小花李琦华
Owner YUNNAN AGRICULTURAL UNIVERSITY
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