Primer pair for detecting rice brown planthopper resistance gene Bph14 and application thereof

A brown planthopper resistance and primer pair technology, applied in the fields of genetic engineering and molecular markers, can solve the problems of complex identification of insect resistance, low efficiency of insect resistance gene transfer, unfavorable breeding efficiency of insect resistance rice, etc. The effect of efficiency

Inactive Publication Date: 2019-06-21
武汉禾泰青生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Due to the complexity of the identification of insect resistance of rice materials, in the backcross breeding of conventional insect-resistant genes, the group identification method at the seedling stage is generally used. Identification to determine contemporary pest resistance

Method used

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  • Primer pair for detecting rice brown planthopper resistance gene Bph14 and application thereof
  • Primer pair for detecting rice brown planthopper resistance gene Bph14 and application thereof
  • Primer pair for detecting rice brown planthopper resistance gene Bph14 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Example 1 Development and Verification of Rice Brown Planthopper Resistance Gene Bph14-specific Co-dominant Molecular Marker

[0070] 1. Development of a specific co-dominant molecular marker for rice brown planthopper resistance gene Bph14

[0071] (1) Perform Standard Nucleotide BLAST analysis on the genome sequence of the fine-mapped segment where Bph14 is located in the NCBI database (https: / / blast.ncbi.nlm.nih.gov / Blast.cgi?PROG RAM=blastn&PAGE_TYPE=BlastSearch&LINK_LOC=blasthome), Comparing the genome sequence of the fine-mapping section where Bph14 is located with the genome sequences of other rice varieties, and listing the specific insertion / deletion fragments in the genome sequence of the Bph14 fine-mapping section; further using Primer 3 online software to design and develop 7 Codominant molecular markers for easy detection (http: / / primer3.ut.ee / ).

[0072] (2) Using the CTAB method (Murray MG&Thompson, 1980 Rapid isolation of high-molecular-weight plant DNA...

Embodiment 2

[0082] Example 2 Application of specific co-dominant molecular marker of rice brown planthopper resistance gene Bph14 in molecular marker-assisted selection breeding

[0083] Using the Bph14-specific co-dominant molecular marker provided in Example 1 of the present invention for molecular marker-assisted selection breeding application, the specific implementation is as follows:

[0084] The excellent restorer line 9311 (susceptible to brown planthopper) widely used in breeding was used as the female parent, and the Bph14 parent material RI35 (derived from B5, without Bph15, containing Bph14, resistant to brown planthopper) was crossed to obtain F 1 ; use 9311 as the parent pair 9311 / RI35F 1 Backcross to get 9311 / / RI35 / 9311BC 1 f 1 . Then, use the Bph14-specific co-dominant molecular marker B14InD developed by the present invention to screen out the backcross offspring containing Bph14, and then use 9311 to carry out backcrossing, and finally screen out the BC containing Bph...

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Abstract

The invention discloses a primer pair for detecting a rice brown planthopper resistance gene Bph14 and an application thereof. The primer pair for detecting the rice brown planthopper resistance geneBph14 is a co-dominance marker primer B14InD. The co-dominance marker primer B14InD is as follows: a forward primer sequence: CTACTGATTGCAGATTGACG; and a reverse primer sequence: CACTTGGTGAACTTATTCCCTT. The primer pair for detecting the rice brown planthopper resistance gene Bph14 is used for performing PCR amplification to obtain a Bph14 specificity co-dominance molecular marker, and the brown planthopper resistance gene positioned by the Bph14 specificity co-dominance molecular marker is clear in position. Through detecting the specificity co-dominance molecular marker of the Bph14 gene, namely genetype detection applied to rice varieties or strains, brown planthopper resistance of a rice plant can be forecasted, and it is beneficial to improve seed breeding efficiency of brown planthopper resistance rice.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to the technical field of molecular markers, in particular to a pair of primers for detecting the brown planthopper resistance gene Bph14 in rice and its application. Background technique [0002] Rice is one of the main food crops in my country, and the brown planthopper is the most serious pest of rice. Both its adults and nymphs can directly suck the phloem juice of rice, resulting in yellow leaves and even death of plants. The outbreaks of brown planthopper populations mostly occurred in the mature grain-filling stage of rice, and the damage area expanded from south to north, the frequency of occurrence increased, and the degree of damage increased. According to the China Agricultural Yearbook, at present, rice planthoppers (mainly brown planthoppers) occur in an area of ​​more than 20 million hectares every year in China, and the annual direct yield loss caused by ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
Inventor 何青杜波祝莉莉王志勇姜伟华闸雯俊
Owner 武汉禾泰青生物科技有限公司
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