Molecular identification method for China rana rugulosa and Thailand rana rugulosa

Abstract

In order to solve the identification problem of China rana rugulosa and Thailand rana rugulosa which have exactly-like appearance during actual protection of China rana rugulosa, the invention provides a molecular identification method for China rana rugulosa and Thailand rana rugulosa. The identification method is based on PCR technology, helps to realize rapid identification on China rana rugulosa and Thailand rana rugulosa by designing a specific primer for amplification, and specifically comprises the steps: 1) extracting DNA of a sample; 2) detecting sample DNA; 3) performing PCR amplification by employing four pairs of specific primers; and 4) performing identification on the specific amplified product. According to the method, by designing the four pairs of primers related to different gene positions, China rana rugulosa and Thailand rana rugulosa can be identified by PCR amplification, the primers are strong in specificity, the agarose gel electrophoresis characteristic spectral band is high in stability and has no regional difference, and the method can help to obtain reliable beneficial effect during practical application.

Description

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AI Technical Summary

Benefits of technology

This patented method uses special techniques called DNA sequencing or polymerase chain reaction (PCR) to accurately detect Japanese tapirflies on their host fishes. By comparing these sequences with existing methods like histological analysis, we found they have strong evidence against certain species such as China's tick-spoons. These experiments showed that this technique could lead us towards understanding how pests live inside caterpillars living around Japan.

Problems solved by technology

This patents describes how tickets like Turtles can hide their appearance when they live outdoors without being seen by humans. These hidden indications may include dirtiness (such as pimples) around certain areas such as those near eyesightedness or behind joint pain syndrome. Additionally, these signs could indicate diseases caused by bacteria called Bacillus cerevisiae which causes swelling inside fishing nerves. Therefore, identifying this type of seafood requires quick and precise techniques to help authorities detect any potential threatening organisms.

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