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Major SNP marker capable of affecting pork aliphatic acid and application of major SNP marker in breeding pigs meat quality heredity improvement

A fatty acid and unsaturated fatty acid technology, which is applied in the direction of recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., can solve problems such as the inability to determine the key mutation site of the main effect gene

Active Publication Date: 2014-07-09
JIANGXI AGRICULTURAL UNIVERSITY
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  • Abstract
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AI Technical Summary

Problems solved by technology

According to the website of the International Porcine QTL Database (http: / / www.animalgenome.org / cgi-bin / QTLdb / SS / index), QTLs that affect fatty acid composition have been located on all pig chromosomes, but most of them use The confidence interval of the QTL located by microsatellite markers is mostly 10-20cM, and the real main effect gene and its key variation sites cannot be determined, so it is difficult to directly apply it to the breeding and improvement of breeding pigs

Method used

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  • Major SNP marker capable of affecting pork aliphatic acid and application of major SNP marker in breeding pigs meat quality heredity improvement
  • Major SNP marker capable of affecting pork aliphatic acid and application of major SNP marker in breeding pigs meat quality heredity improvement
  • Major SNP marker capable of affecting pork aliphatic acid and application of major SNP marker in breeding pigs meat quality heredity improvement

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Embodiment 1

[0027] This example specifically explains the inventive process for obtaining the SNP site of the present invention.

[0028] Detection of 60,000 (60K) SNP genotypes of the whole swine genome: A small piece of ear tissue sample was collected from each individual in the above 3 experimental groups, and the whole genome DNA was extracted by the standard phenol-chloroform method, after Nanodrop-100 spectrophotometry After measuring the quality, the concentration was uniformly diluted to 50ng / ul, and the whole swine genome 60K SNP (Illumina, USA) genotype was determined on the Illumina Beadstation platform according to the company's standard process. Use PLINK (1.07) to perform quality control on the 60K chip data of all samples, and eliminate individuals with a detection rate of less than 95% and a family Mendelian error rate of more than 0.1; and the detection rate of less than 95%, and the minimum allele frequency less than 0.05 and Hardy-Weinberg balance is significantly higher t...

Embodiment 2

[0047] This example is the SNP site obtained in Example 1. 120963718C> T and / or g.120963819C> Application of G in breeding pig breeding. For individuals in the nucleus of breeding pigs, the above method of direct sequencing after PCR amplification was used to detect the g.120963718 and g.120963819 genotypes of the two major mutation sites on pig chromosome 14 that affect the content of pig muscle C18:0. The selection is advantageous. Genotypes TT and GG are reserved for individuals. After the subsequent selection of the population, the unsaturated fatty acid content of the pig herd is increased while the saturated fatty acid content is reduced, and the quality and health index of pork are improved.

[0048] Specifically, the Western ternary hybrid commercial pig-Duroc × (Big White × Landrace) commercial pig is used as the experimental animal. 602 Duroc pigs × (Large White × Landrace) ternary commodity pigs were slaughtered in multiple batches at the Jiangxi Guohong Company slaugh...

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Abstract

The invention provides a major SNP marker capable of affecting pork aliphatic acid component, the SNP marker is positioned on a nucleotide sequence of pig SCD gene, the site marked by SNP is g.120963718 nucleotide site on international pig genome version 10.2 reference sequence pig No.14 chromosome, and is the mutation of C and T, which is corresponded to 4513rd site on SEQ ID NO: 1, the position marked by SNP is g.120963819 nucleotide site on international pig genome version 10.2 reference sequence pig No.14 chromosome, and is the mutation of C and G, which is corresponded to 4614th site on SEQ ID NO: 1, above two mentioned sites are completely linked; the SNP marker can increase the monounsaturated fatty acid content and / or reduce saturated fatty acid content of pig, the monounsaturated fatty acid is C18: 1 and / or C16: 1, and the saturated fatty acid is C18: 0 and / or C16: 0. The invention also provides a nucleotide sequence of SCD gene positioned on pig No.14 chromosome, and the application of the SNP marker and the nucleotide sequence in breeding pigs meat quality heredity improvement.

Description

Technical field [0001] The present invention relates to the fields of molecular biotechnology and animal genetics and breeding, in particular to identifying major gene markers that can significantly increase the content of unsaturated fatty acids C18:1 and C16:1 in commercial pig muscle while reducing the content of saturated fatty acids C18:0 and C16:0 and Its application in genetic improvement of breed pork quality. Background technique [0002] Fatty acid refers to a long fatty hydrocarbon chain with a carboxyl group at one end, and is the main component of animal and plant adipose tissue, phospholipids and glycolipids. According to the number of double bonds in the carbon chain, fatty acids can be divided into saturated fatty acids (without double bonds, SFA), monounsaturated fatty acids (with only one double bond, MUFA) and polyunsaturated fatty acids (containing multiple double bonds, PUFA) ). Pork contains 18 common fatty acids, the most abundant of which are C18:1, C16:...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 任军杨斌麻骏武张万昌黄路生
Owner JIANGXI AGRICULTURAL UNIVERSITY
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