Red camellia extract-containing anti-wrinkle composition for promoting cell energy and application thereof

Abstract

The invention discloses an anti-wrinkle composition containing a camellia japonica extract and capable of promoting cell energy and an application of the anti-wrinkle composition. The anti-wrinkle composition is prepared from the following components in percentage by mass: 5 to 20 percent of camellia japonica extract, 7.4 to 7.6 percent of flaxseed oil, 0.14 to 0.16 percent of sorbitan trioleate, 0.005 to 0.007 percent of celery seed extract, 0.004 to 0.006 percent of crithmum maritimum extract, 0.003 to 0.004 percent of flaxseed extract and the balance of caprylic / capric triglyceride which is supplemented to 100 percent. The invention further discloses application of the anti-wrinkle composition in promoting synthesis of NAD < + > and ATP in dermal fibroblasts and / or epidermal keratinocytes and / or SIRT3 gene expression and application of the anti-wrinkle composition in preparing cosmetics or skin care products with anti-wrinkle and tightening effects. The composition disclosed by the invention is an oil-soluble composition, is easier to absorb, and can improve the mitochondrial activity through synergistic interaction.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, in particular to an anti-wrinkle composition containing red camellia extract and capable of promoting cell energy and its application. Background Art

[0002] Skin is the body's largest organ, covering the entire body. It protects various tissues and organs from external environmental damage. However, with aging, environmental pollution, radiation from electronic devices, and other factors, the skin's protective barrier is damaged, leading to aging symptoms such as loss of elasticity, wrinkles, and dullness. Research has shown that continuous renewal and repair of skin tissue is crucial for maintaining youthful appearance, but adult stem cells have a limited number of divisions, necessitating the use of skincare cosmetics.

[0003] Mitochondria are double-membrane organelles with autonomous genomes, found in the cytoplasm of nearly all eukaryotic cells. They possess a single mtDNA (mitochondrial DNA). They are the site of cellular respiration and the production of most adenosine triphosphate (ATP). ATP is a high-energy, small molecule intracellular compound often referred to as the "molecular currency" of intracellular energy transfer. It is the direct source of energy for various biological activities. Increased cellular ATP levels promote cell repair and regeneration, and the level of cellular ATP directly reflects cell vitality. For example, fibroblasts are cells in the skin that synthesize proteins such as collagen. This protein synthesis process consumes significant amounts of energy, and the energy for cellular activity comes from ATP produced by mitochondria. Therefore, the more efficient the energy provided, the faster and more abundant the fibroblasts can synthesize protein.

[0004] Nicotinamide adenine dinucleotide (NAD+) serves as a cofactor for key enzymes in glycolysis, the tricarboxylic acid cycle, and oxidative phosphorylation, participating in a variety of physiological and pathological processes, including cellular metabolism, energy synthesis, and DNA repair. In recent years, a growing number of studies have found that intracellular NAD+ levels show a significant downward trend during aging. This decrease in NAD+ levels leads to decreased activity of SIRT3, a major mitochondrial deacetylase. SIRT3 participates in the electron transport chain that produces ATP, thereby regulating ATP levels.

[0005] Patent application CN118750401A discloses a composition for improving mitochondrial function, a care product and use. The composition for improving mitochondrial function comprises arbutin and a mitochondrial function improver, wherein the mitochondrial function improver comprises one or more of glabridin, ergothioneine. The composition can effectively improve mitochondrial function to achieve the purpose of cell protection and relieve skin aging problems.

[0006] Patent application CN118986781A discloses a composition for improving mitochondrial activity and its application in cosmetics. The composition is compounded from hyaluronic acid lysine copper chelate and otsu cocoa extract. The test verifies that the composition can significantly promote NAD+, mitochondrial ATP expression, promote Collagen III expression, and inhibit mitochondrial ROS expression, has good effects of improving mitochondrial activity and effectively resisting aging, and can be used in cosmetics to improve skin relaxation and wrinkle problems.

[0007] Patent CN116115655B relates to the use of pine bark and extracts thereof in the preparation of pharmaceutical compositions for preventing and / or treating diseases and / or symptoms in a subject that benefit from increased NAD levels. It also relates to the use of pine bark and extracts thereof in the preparation of compositions for increasing NAD levels in at least one tissue of a subject.

[0008] Patent application CN118436562A relates to a composition for improving mitochondrial autophagy function and its application, belonging to the technical field of cosmetics. The composition of the provided cordyceps militaris fermentation liquor, tetrahydro methyl pyrimidine carboxylic acid and glass acid dimethyl silanol ester complex can promote mitochondrial autophagy and improve mitochondrial function, thereby improving skin condition. The cordyceps militaris fermentation liquor is obtained by liquid fermentation of mycelium, which replaces wild cordyceps militaris picking with artificial cultivation fermentation method, realizes sustainable development of rare plant resources, and reduces cost. Compared with traditional cordyceps militaris fruiting body extract, the cordyceps militaris fermentation liquor has short production cycle, high batch stability, simple preparation process, is convenient for large-scale industrial production, does not need external extraction solvent, is more green and environmentally friendly, and is more conducive to enrichment of active ingredients.

[0009] However, the active compositions for improving mitochondrial function in the above patents are water-soluble, while the skin is lipophilic, so there is still a lack of oil-soluble natural plant extracts that can promote mitochondrial activity in keratinocytes and fibroblasts in the dermis and epidermis. SUMMARY

[0010] To solve the above technical problems, the purpose of the present application is to provide an anti-wrinkle composition containing camellia sinensis leaf extract for promoting cell energy.

[0011] The object of the present invention is achieved through the following technical solutions: In a first aspect, the present invention provides an anti-wrinkle composition for promoting cellular energy containing a Camellia sinensis extract, comprising the following components by mass percentage: Red Camellia Extract 5-20%, Flaxseed oil 7.4-7.6%, Sorbitan trioleate 0.14-0.16%, Celery seed extract 0.005-0.007%, Sea fennel extract 0.004-0.006%, Flaxseed extract 0.003-0.004%, It was supplemented to 100% with caprylic / capric triglyceride.

[0012] As some specific embodiments of the present invention, the following components are included in percentage by mass: Red Camellia Extract 5-20%, 7.5% linseed oil, Sorbitan trioleate 0.15%, Celery seed extract 0.006%, Sea fennel extract 0.005%, Flaxseed extract 0.00375%, It was supplemented to 100% with caprylic / capric triglyceride.

[0013] As some specific embodiments of the present invention, the red camellia extract includes red camellia seed oil, red camellia flower extract and red camellia leaf extract, and the mass ratio of the red camellia seed oil, red camellia flower extract and red camellia leaf extract is 75-90:5-15:5-15, preferably 88:6.5:5.5.

[0014] As some specific embodiments of the present invention, the preparation method of the Camellia japonica extract or Camellia sinensis leaf extract comprises: crushing and sieving the Camellia japonica or Camellia sinensis leaf, and then performing supercritical CO2 extraction to obtain the extract.

[0015] As some specific embodiments of the present invention, the parameters of the supercritical extraction include: extraction pressure of 15-30 MPa; extraction temperature of 30-50° C.; and extraction time of 1-2 h.

[0016] As some specific embodiments of the present invention, the preparation method of red camellia seed oil specifically comprises the following steps: A. Heat the squeezed red camellia seed crude oil to 40-95°C, add the alkaline solution under stirring, stop stirring after soap particles are formed, keep the temperature and let it stand, and remove the soap stock and water; B. The red camellia seed oil obtained after the treatment is washed with water by adding a saline solution under stirring, and then allowed to stand to remove colloidal impurities and water; C. Vacuum dehydration is then performed, wherein the red camellia seed oil obtained after the treatment in step B is heated under vacuum conditions to be dehydrated; D. Decolorization: cooling the red camellia seed oil treated in step C, adding a decolorizing agent under stirring to decolorize the oil, and filtering to obtain the decolorized red camellia seed oil; E. Inert gas steam deodorization: The decolorized red camellia seed oil is introduced into an inert gas under vacuum conditions, stirred and heated; when the temperature rises to 130°C-250°C, steam is introduced for deodorization; then the steam is turned off and the temperature is lowered to obtain the deodorized red camellia seed oil; F. Winterization: Cool the deodorized red camellia seed oil and then keep it warm, then remove wax and solid impurities to obtain the red camellia seed oil.

[0017] In some specific embodiments of the present invention, the linseed oil is obtained by squeezing and filtering linseeds without using any solvents, additives, antioxidants or other synthetic substances.

[0018] As some specific embodiments of the present invention, the preparation method of the flaxseed extract includes: grinding flaxseed (using an oil press), extracting with an ethanol-water solution, stirring to separate the suspension into two phases, standing for 3-5 days to remove the oily component, vacuum evaporating the ethanol, collecting the solid residue and drying and grinding it into a fine powder.

[0019] As some specific embodiments of the present invention, the preparation method of the celery seed extract includes: crushing the celery seeds to obtain a powder with a particle size of 700-900 μm, performing supercritical extraction with CO2 at a pressure of 80-100 bar and a temperature of 35-45°C; and removing residual water to obtain the extract in the form of an oily liquid.

[0020] As some specific embodiments of the present invention, the preparation method of the sea fennel extract includes: drying the organic sea fennel and then performing supercritical extraction with CO2, the extraction pressure is 20-50 MPa, and the temperature is 25-50°C.

[0021] In a second aspect, the present invention provides a use of the anti-wrinkle composition as described in any one of the above items in promoting the synthesis of NAD+, ATP and / or SIRT3 gene expression in dermal fibroblasts and / or epidermal keratinocytes.

[0022] In a third aspect, the present application provides use of the composition according to any one of the above aspects in the preparation of a cosmetic or skin care product having anti-wrinkle and firming effects. As a key coenzyme, NAD+ is involved in the three major metabolic pathways of glycolysis, tricarboxylic acid cycle (TCA cycle) and oxidative phosphorylation, and converts nutrients into ATP (the direct energy currency of cells). When ATP is increased, cells can more efficiently perform collagen synthesis, thereby achieving anti-wrinkle and firming effects.

[0023] Compared with the prior art, the present application has the following beneficial effects: (1) The present application provides an oil-soluble composition capable of effectively increasing the levels of NAD+, SIRT3 and ATP in dermal fibroblasts and epidermal keratinocytes.

[0024] (2) Due to the lipophilicity of the skin, the oil-soluble composition is more easily absorbed than the water-soluble composition. Moreover, through the compounding of different components within a certain proportion range, a synergistic effect is achieved, which significantly increases the activity of mitochondria, and ultimately achieves anti-wrinkle and firming effects. BRIEF DESCRIPTION OF DRAWINGS

[0025] Other features, objects and advantages of the present application will become more apparent from the following detailed description of non-limiting embodiments, made with reference to the following drawings: Figure 1 Figure 1 is a graph showing the changes in NAD+ content in epidermal keratinocytes treated with the sample of each example and the comparative example; Figure 2 Figure 2 is a graph showing the changes in the relative expression amount of SIRT3 in epidermal keratinocytes treated with the sample of each example and the comparative example; Figure 3 Figure 3 is a graph showing the changes in ATP content in epidermal keratinocytes treated with the sample of each example and the comparative example; Figure 4 Figure 4 is a graph showing the changes in NAD+ content in dermal fibroblasts treated with the sample of each example and the comparative example; Figure 5 Figure 5 is a graph showing the changes in the relative expression amount of SIRT3 in dermal fibroblasts treated with the sample of each example and the comparative example; Figure 6 Figure 6 is a graph showing the changes in ATP content in dermal fibroblasts treated with the sample of each example and the comparative example. DETAILED DESCRIPTION

[0026] The application will be described in detail below with specific examples. The following examples will help those skilled in the art to further understand the application, but do not limit the application in any form. It should be pointed out that those skilled in the art can make several modifications and improvements without departing from the concept of the application. These all belong to the protection scope of the application.

[0027] The components used in the following examples and comparative examples were prepared by the following method.

[0028] 1. Camellia sinensis var. assamica extract It includes camellia sinensis var. assamica seed oil, camellia sinensis var. assamica flower extract and camellia sinensis var. assamica leaf extract, and the mass ratio of the three is 88:6.5:5.5.

[0029] The preparation method of camellia sinensis var. assamica flower extract and camellia sinensis var. assamica leaf extract is: after the camellia sinensis var. assamica flower and the camellia sinensis var. assamica leaf are respectively crushed and sieved, supercritical CO2 extraction is carried out, wherein the extraction pressure is 20 Mpa; the extraction temperature is 35 ℃; and the extraction time is 2 h.

[0030] The preparation method of camellia sinensis var. assamica seed oil refers to patent CN111218335B, and specifically includes the following steps: A) The pressed camellia sinensis var. assamica seed crude oil is heated to 50 ℃, and an alkali solution is added under the condition of stirring at 50 r / min. After the formation of soap particles, stirring is stopped, and the solution is kept and placed. The soap foot and water are removed; B) The camellia sinensis var. assamica seed oil obtained after treatment is added to a salt solution under the condition of stirring at 60 r / min for water washing, and then the colloidal impurities and water are removed after standing; C) Then vacuum dehydration is carried out. The camellia sinensis var. assamica seed oil obtained after step B is heated to 50 ℃ under the condition of vacuum at 0.95 Kpa; D) Decolorization: After the camellia sinensis var. assamica seed oil treated in step C is cooled, active white clay and activated carbon are added under the condition of stirring for decolorization. After filtration, decolorized camellia sinensis var. assamica seed oil is obtained; E) Inert gas protection steam deodorization: The decolorized camellia sinensis var. assamica seed oil is stirred and heated for about 3 h under vacuum, and then water vapor is introduced for deodorization when the temperature rises to 250 ℃. Then the water vapor is turned off, and the temperature is lowered to obtain deodorized camellia sinensis var. assamica seed oil; F) Winterization: The deodorized camellia sinensis var. assamica seed oil is cooled to 1 ℃, kept for 2 h, and then the wax and solid impurities are removed to obtain the camellia sinensis var. assamica seed oil.

[0031] 2. Linseed oil: The dried linseed is sent into a press for pressing. The product is filtered to remove impurities and precipitates, and the oil phase obtained is the linseed oil.

[0032] 3. Linseed extract: Linseed was ground using an oil press, extracted with aqueous ethanol, the suspension was stirred to separate into two phases, left to stand for 3-5 days, the oily components were removed, the ethanol was evaporated under vacuum, the solid residue was collected and ground to a fine powder. The preparation of the linseed extract described above is described in patent CN111670027B.

[0033] 4. Osmunda cinnamomea extract: Osmunda cinnamomea seeds were ground to a powder of 800 pm particle size, supercritical extraction was performed using CO2 at a pressure of 90 bar and a temperature of 40 °C. After removal of residual water, the extract was obtained in the form of an oily liquid. See patent WO 2016 / 157073 A1 for details.

[0034] 5. Sea fennel extract: Sea fennel was dried and supercritical extraction was performed using CO2 at a pressure of 30 Mpa and a temperature of 35 °C.

[0035] The present application provides the following examples and comparative examples: Example 1 This example provides a plant extract composition comprising the following components in mass percentage: Camellia sinensis extract 20%, Linseed oil 7.5%, Sorbitan trioleate 0.15%, Osmunda cinnamomea extract 0.006%, Sea fennel extract 0.005%, Linseed extract 0.00375%, Caprylic / capric triglyceride to 100%.

[0036] Example 2 This example provides a plant extract composition comprising the following components in mass percentage: Camellia sinensis extract 10%, Linseed oil 7.5%, Sorbitan trioleate 0.15%, Osmunda cinnamomea extract 0.006%, Sea fennel extract 0.005%, Linseed extract 0.00375%, Caprylic / capric triglyceride to 100%.

[0037] Example 3 This example provides a plant extract composition comprising the following components in mass percentage: Camellia sinensis extract 5%, 7.5% linseed oil, Sorbitan trioleate 0.15% Celery seed extract 0.006%, Sea fennel extract 0.005%, Flaxseed extract 0.00375%, Caprylic / capric triglyceride supplemented to 100%.

[0038] Comparative Example 1 This comparative example provides a plant extract composition, which comprises the following components in percentage by mass: Red Camellia Extract 2.5%, 7.5% linseed oil, Sorbitan trioleate 0.15%, Celery seed extract 0.006%, Sea fennel extract 0.005%, Flaxseed extract 0.00375%, Caprylic / capric triglyceride supplemented to 100%.

[0039] Comparative Example 2 This comparative example provides a plant extract composition, which comprises the following components in percentage by mass: Red Camellia Extract 25%, 7.5% linseed oil, Sorbitan trioleate 0.15%, Celery seed extract 0.006%, Sea fennel extract 0.005%, Flaxseed extract 0.00375%, Caprylic / capric triglyceride supplemented to 100%.

[0040] Comparative Example 3 This comparative example provides a plant extract composition, which lacks linseed oil based on Example 2. Calculated by mass percentage, it includes the following components: Red Camellia Extract 10%, Sorbitan trioleate 0.15%, Celery seed extract 0.006%, Sea fennel extract 0.005%, Flaxseed extract 0.00375%, Caprylic / capric triglyceride supplemented to 100%.

[0041] Comparative Example 4 This comparative example provides a plant extract composition which lacks sorbitan trioleate on the basis of Example 2. It comprises the following components in mass percent: Camellia sinensis extract 10%, Linseed oil 7.5%, Oenothera biennis extract 0.006%, Sea fennel extract 0.005%, Linseed extract 0.00375%, Caprylic / capric triglyceride to 100%.

[0042] Comparative Example 5 This comparative example provides a plant extract composition which lacks linseed extract on the basis of Example 2. It comprises the following components in mass percent: Camellia sinensis extract 10%, Linseed oil 7.5%, Sorbitan trioleate 0.15%, Oenothera biennis extract 0.006%, Sea fennel extract 0.005%, Caprylic / capric triglyceride to 100%.

[0043] Comparative Example 6 This comparative example provides a plant extract composition which lacks caprylic / capric triglyceride on the basis of the Examples and is made up to 100 wt.% with Camellia sinensis extract. It comprises the following components in mass percent: Camellia sinensis extract 92.33525%, Linseed oil 7.5%, Sorbitan trioleate 0.15%, Oenothera biennis extract 0.006%, Sea fennel extract 0.005%, Linseed extract 0.00375%.

[0044] The components of Examples 1-3 and Comparative Examples 1-6 are listed in Table 1 below: Table 1: Set-up of Examples and Comparative Examples

[0045] Effect Example 1 Epidermal keratinocytes Human epidermal keratinocytes (HaCaT) were used as the research object, and an in vitro model was established by H2O2 stimulation, and the compositions of each example and comparative example were treated, and the changes of NAD+, ATP content and relative expression amount of SIRT3 in each group were detected, and the blank control group (control group, BC group) was not treated; the negative control group (NC) was only treated with H2O2 without applying the composition.

[0046] 1. Test method 1) Cell inoculation: HaCaT cells were inoculated into a 24-well plate at a seeding density of 1×10 5 The HaCaT cells were inoculated into a 24-well plate at a seeding density of 1×10

[0047] 2) Add test substance: according to the results of the cell toxicity experiment, add the test substance according to the experimental grouping design table (Table 2), when the cell plating rate in the 24-well plate reaches 40%-60%, add the test substance in groups, set 3 replicate wells for each group, and place the 24-well plate in an incubator (37℃, 5% CO2) for incubation for 24h.

[0048] 3) Detection: (a) After adding H2O2 for 2h, discard the supernatant, rinse with PBS, and then use the corresponding lysis buffer (Shanghai Biyun Tian Biotechnology Co., Ltd. P0013B) to lyse the cells. After lysis, centrifuge at 4℃, 12000g for 5min, and take the supernatant to use the enhanced ATP detection kit (Shanghai Biyun Tian Biotechnology Co., Ltd. S0027) and the enhanced NAD+ / NADH detection kit (WST-8 method) (Shanghai Biyun Tian Biotechnology Co., Ltd. S0176S) to respectively determine the contents of ATP and NAD+.

[0049] (b) After adding H2O2 for 2h, discard the supernatant, rinse with PBS, and then add 0.5mL lysis buffer to each well, use the cell RNA extraction kit (Yixing Biotechnology (Shanghai) Co., Ltd. 19231ES50) to extract RNA, and after reverse transcription to cDNA (reverse transcription kit II—MCE HY-K0511A), perform RT-qPCR (SYBR probe—Genstar A301-10) detection, and calculate the results.

[0050] Table 2 Experimental grouping design table

[0051] 2. Test results Table 3 NAD+ content detection results

[0052] The NAD+ content detection results of epidermal keratinocytes after treatment with different samples are as follows:Figure 1 as shown.

[0053] According to Table 3 and Figure 1 , it can be seen that compared with the NC group of stimulating HaCaT cells with only hydrogen peroxide, the NAD+ content of the cells treated with the plant extract composition in advance in Examples 1-3 is generally increased by more than 45%, while the increase rate of the comparative examples is mostly less than 10%.

[0054] Table 4 SIRT3 gene relative expression amount result table

[0055] The relative expression amount of SIRT3 gene in epidermal keratinocytes after treatment of different samples is shown in Figure 2 .

[0056] According to Table 4 and Figure 2 , it can be seen that the relative expression amount of SIRT3 gene in HaCaT cells is also more obviously promoted by the examples.

[0057] Table 5 ATP content result table

[0058] The detection result of ATP content in epidermal keratinocytes after treatment of different samples is shown in Figure 3 .

[0059] According to Table 5 and Figure 3 , it can also be seen that the synthesis of ATP in HaCaT cells is better promoted by each example.

[0060] 3. Experimental conclusion According to Tables 3, 4, 5 and Figure 1 , 2 , 3, it can be seen that under the experimental conditions, compared with the NC group, whether it is NAD+ content, SIRT3 relative gene expression amount or ATP content, Examples 1, 2 and 3 all have a good increase rate, indicating that the composition of the present application can improve the mitochondrial activity of epidermal keratinocytes, thereby achieving the repair effect; among them, the effect of Example 2 is the most significant, Comparative Example 1 has almost no effect, and Comparative Example 2 also has a certain effect, but the content of Hongchacha extract is too high, and the effect is obviously not as good as Examples 1-3, and the cost performance is low.

[0061] Effect Example 2 dermal fibroblasts Take human normal skin fibroblasts (HSF) as the research object, and establish an in vitro model by stimulating with doxorubicin DOX, and then treat with the compositions of each example and the comparative example, detect the changes of NAD+ and ATP contents and the relative expression amount of SIRT3 in each group, and set up a blank control group (control group, BC group) without treatment; a negative control group (NC) is only treated with doxorubicin DOX without applying the composition.

[0062] 1. Test method 1) Cell inoculation: after the HSF cells in the logarithmic growth phase were washed twice with PBS, trypsin was added for digestion, and after the digestion was terminated by adding DMEM medium (10% FBS), centrifugation was performed, the supernatant was discarded, and DMEM medium (10% FBS) was added to prepare a cell suspension. The cell suspension was added to cell culture dishes, which were placed in a cell culture incubator for culture until the cell density was 80%-90% for treatment.

[0063] 2) Add test objects: the blank control group is not treated, the model control group is treated with DOX, and the sample group is added with DOX and DMEM medium (10% FBS) of corresponding concentration according to the following Table 6.

[0064] 3) Detection: (a) After continuing to culture in a 37°C, 5% CO2 cell culture incubator for 72 h, the culture medium was discarded, the cells were collected into centrifuge tubes, centrifuged, the supernatant was discarded, and the extraction solution was added for ice bath ultrasonic cell disruption, low-temperature centrifugation was performed to collect the supernatant. NAD+ / NADH ratio detection kit (Shanghai Biyun Tian Biotechnology Co., Ltd. S0175) and ATP detection kit (Jianglai Biology JL-T0632-48 sample) were used to detect the contents of NAD+ and ATP.

[0065] (b) After continuing to culture in a 37°C, 5% CO2 cell culture incubator for 24 h, the RNA of each group of cells was extracted (Trizol-Summerfly 15596018), reverse transcribed into cDNA (Reverse Transcription Kit II-TOYOBO FSQ-301), and the expression level of SIRT3 gene in each group of cells was detected by RT-qPCR method (SYBR Green fluorescent dye-TOYOBO FSQ-201).

[0066] Table 6: Experimental grouping design table

[0067] 2. Test results Table 7: NAD+ content results

[0068] The changes of NAD+ content in the dermal fibroblasts after different sample treatments are shown in Table 7. Figure 4

[0069] Table 8 SIRT3 gene relative expression rate results

[0070] The changes of SIRT3 relative expression in the dermal fibroblasts after different sample treatments are shown in Table 8. Figure 5

[0071] Table 9 ATP content results

[0072] The changes of ATP content in the dermal fibroblasts after different sample treatments are shown in Table 9. Figure 6

[0073] 3、Experimental conclusion According to Tables 7-9 and Figure 4 , 5 , 6, under the experimental conditions, relative to the NC group, whether it is NAD+ content, SIRT3 relative gene expression or ATP content, examples 1-3 all have a good improvement rate, indicating that the mitochondrial activity of dermal fibroblasts can be improved, thereby achieving the effect of anti-wrinkle and firming. Among them, the effect of example 2 is the most significant, and the comparative examples 1 and 3-6 have almost no significant effect, especially for the promotion effect of SIRT3 relative gene expression, the difference between the examples and the comparative examples is very significant. Although the effect of comparative example 2 is better than that of other comparative examples, it is still significantly inferior to the examples, and the proportion of Hongchacha extract in comparative example 2 is improved based on example 2, but the effect is significantly reduced.

[0074] The specific embodiments of the present application are described above. It should be understood that the present application is not limited to the above specific embodiments, and those skilled in the art can make various modifications or modifications within the scope of the claims, which does not affect the essential content of the present application.​​​

Claims

1. An anti-wrinkle composition for promoting cell energy containing red camellia extract, characterized in that: Calculated by mass percentage, it includes the following components: Red Camellia Extract 5-20%, Flaxseed oil 7.4-7.6%, Sorbitan trioleate 0.14-0.16%, Celery seed extract 0.005-0.007%, Sea fennel extract 0.004-0.006%, Flaxseed extract 0.003-0.004%, It was supplemented to 100% with caprylic / capric triglyceride.

2. The anti-wrinkle composition according to claim 1, characterized in that Calculated by mass percentage, it includes the following components: Red Camellia Extract 5-20%, 7.5% linseed oil, Sorbitan trioleate 0.15%, Celery seed extract 0.006%, Sea fennel extract 0.005%, Flaxseed extract 0.00375%, It was supplemented to 100% with caprylic / capric triglyceride.

3. The anti-wrinkle composition according to claim 1 or 2, characterized in that The red camellia extract comprises red camellia seed oil, red camellia flower extract and red camellia leaf extract, and the mass ratio of the red camellia seed oil, red camellia flower extract and red camellia leaf extract is 75-90:5-15:5-15.

4. The anti-wrinkle composition according to claim 3, characterized in that The preparation method of the red camellia flower extract or the red camellia tea extract comprises the following steps: crushing and sieving the red camellia flower or the red camellia tea, and then performing supercritical CO2 extraction to obtain the extract.

5. The anti-wrinkle composition according to claim 4, characterized in that The parameters of the supercritical extraction include: extraction pressure of 15-30 MPa; extraction temperature of 30-50° C.; and extraction time of 1-2 h.

6. The anti-wrinkle composition according to claim 3, characterized in that The preparation method of the red camellia seed oil specifically comprises the following steps: A. Heat the squeezed red camellia seed crude oil to 40-95°C, add the alkaline solution under stirring, stop stirring after soap particles are formed, keep the temperature and let it stand, and remove the soap stock and water; B. The red camellia seed oil obtained after the treatment is washed with water by adding a saline solution under stirring, and then allowed to stand to remove colloidal impurities and water; C. Vacuum dehydration is then performed, wherein the red camellia seed oil obtained after the treatment in step B is heated under vacuum conditions to be dehydrated; D. Decolorization: cooling the red camellia seed oil treated in step C, adding a decolorizing agent under stirring to decolorize the oil, and filtering to obtain the decolorized red camellia seed oil; E. Inert gas steam deodorization: The decolorized red camellia seed oil is introduced into an inert gas under vacuum conditions, stirred and heated; when the temperature rises to 130°C-250°C, steam is introduced for deodorization; then the steam is turned off and the temperature is lowered to obtain the deodorized red camellia seed oil; F. Winterization: Cool the deodorized red camellia seed oil and then keep it warm, then remove wax and solid impurities to obtain the red camellia seed oil.

7. The anti-wrinkle composition according to claim 1 or 2, characterized in that The linseed oil is obtained by pressing and filtering linseeds; And / or, the preparation method of the flaxseed extract comprises: grinding flaxseed, extracting with ethanol-water solution, stirring to separate the suspension into two phases, standing for 3-5 days to remove oily components, evaporating ethanol under vacuum, collecting the solid residue and drying and grinding it into a fine powder.

8. The anti-wrinkle composition according to claim 1 or 2, characterized in that The preparation method of the celery seed extract comprises: crushing the celery seeds to obtain a powder with a particle size of 700-900 μm, performing supercritical extraction with CO2 at a pressure of 80-100 bar and a temperature of 35-45°C; and removing residual water to obtain the extract; And / or, the preparation method of the sea fennel extract comprises: drying the organic sea fennel and then performing supercritical extraction with CO2, the extraction pressure being 20-50 MPa and the temperature being 25-50°C.

9. Use of the anti-wrinkle composition according to any one of claims 1 to 8 in promoting the synthesis of NAD+ and ATP and / or SIRT3 gene expression in dermal fibroblasts and / or epidermal keratinocytes.

10. Use of the anti-wrinkle composition according to any one of claims 1 to 8 in the preparation of cosmetics or skin care products with anti-wrinkle and firming effects.

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