Starfish extract used for preparing composition for promoting collagen proliferation

A collagen and extract technology is applied in the directions of drug combinations, cosmetic preparations, medical raw materials derived from echinoderms, etc., to achieve the effect of simple preparation method and promotion of production

Inactive Publication Date: 2015-05-20
百岳特生物科技(上海)有限公司 +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of this, improving the collagen production ability of human skin fibroblasts can ensure the effective production and utilization of collagen than supplementing collagen. However, there is currently no safe and effective product on the market that can promote collagen production. generate

Method used

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  • Starfish extract used for preparing composition for promoting collagen proliferation
  • Starfish extract used for preparing composition for promoting collagen proliferation
  • Starfish extract used for preparing composition for promoting collagen proliferation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: the preparation of starfish extract

[0019] The flying white maple starfish (Archaster typicus) used in the present invention is collected from the Penghu area of ​​Taiwan. For the preparation of starfish extract, please refer to figure 1 . When preparing the starfish extract of the present invention, first, collect the living starfish and immediately store it in a frozen environment at -4°C to -20°C when it is captured, and grind the frozen live starfish (such as step S1); then, grind the starfish Dissolve starfish in water at a ratio of 1:3 to 1:20 (w:v) and heat to 90°C to 100°C for extraction for 1 to 5 hours (as in step S2); after high temperature extraction, filter by centrifugation (4,300g) And take its supernatant (as in step S3); then, add 3 times the volume of ethanol at 4°C to precipitate the polysaccharide and maintain it for 16-24 hours (as in step S4); after the ethanol precipitation, centrifuge (4,300g rpm) filter and take its precipitat...

Embodiment 2

[0020] Example 2: Analysis of water-soluble polysaccharides from starfish extract

[0021] The total polysaccharide content of the starfish extract of the present invention is determined by a phenol-sulfuric acid method. 100 μL starfish extract was mixed with different concentrations of glucose standard solutions (0, 25, 50, 75, 100 and 125 mg / mL) in a test tube. Then add 0.5mL phenol solution and 2.5mL sulfuric acid to react. Measure the absorbance of the test tube at 750 nm with a spectrophotometer.

[0022] Analysis result shows, contains the water-soluble polysaccharide body of 2.23% (w / v) in the starfish extract solution of 2.5% (w / v), therefore it can be known that the contained water-soluble polysaccharide body content of starfish extract of the present invention is about 89% (w / w).

Embodiment 3

[0023] Example 3: Analysis of the ability of starfish extract to promote collagen proliferation

[0024] Human skin fibroblast cells CCD-966SK (Human skin fibroblast cells, purchased from Food Industry Development Institute) were cultured in 24-well plates with minimum essential medium (MEM) containing 10% fetal bovine serum (FBS). The concentration of mother cells was 4 x 10 4 cells / well. The medium was refreshed after 24 hours of incubation. In the experimental group, starfish extract was diluted with phosphate buffer saline (PBS) (concentrations were 0.2, 0.4, 0.6, 0.8, and 1.0 mg / mL) and added to each well. The control group did not add starfish extract, and cultured at 37°C and 5% carbon dioxide for 48 hours and then took its supernatant. The experiment found that starfish extract promoted collagen production within 48 hours and there was a linear correlation with time. .

[0025] use Collagen Assay Kit (Bicolor Life Science Assays, Northern Ireland, UK) was used to...

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Abstract

The invention discloses a starfish extract used for preparing a composition for promoting the collagen proliferation, which comprises a variety of active ingredients used for effectively improving the collagen generation capability of dermal fibroblasts cells. Meanwhile, the extraction method of the invention is capable of improving the economic worth of a starfish and is pollution-free. Meanwhile, the starfish extract can be effectively applied to skin-care products and cosmetics.

Description

technical field [0001] The present invention relates to an application of promoting collagen proliferation, in particular to the application of a starfish extract in preparing a composition for promoting collagen proliferation. Background technique [0002] Starfish is the name of a class of animals under the phylum Echinodermata. It has amazing regeneration ability, not only can regenerate broken arms and legs, some starfish can even regenerate a complete new individual from the amputated arms and legs themselves. Starfish are mostly distributed in the coastal areas of Asia, North America, and Australia, and their habitats are usually sandy and muddy bottoms. Taiwan is an island, rich in aquatic products, and there are also many kinds of starfish, such as the flying white maple starfish (Archaster typicus) that is common in the intertidal zone of Penghu. Starfish has a wide range of uses. In addition to being used as ingredients or processed food materials, some starfish a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/616A61K8/98A61Q19/00A61P17/16A61Q17/04A61Q19/10A61Q1/08
Inventor 苏香绫林咏翔林咏凯陈盈如
Owner 百岳特生物科技(上海)有限公司
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