Serum-free culture medium for dental pulp stem cells
A technology of serum-free medium and dental pulp stem cells, which is applied in the field of stem cell culture, can solve problems such as unclear components, and achieve the effects of clear components, cost saving, and easy operation
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[0026] Embodiment 1 A kind of serum-free culture medium
[0027] (Homemade) A serum-free medium for dental pulp stem cells including growth factors, hormones, proteins, vitamins and reducing substances, adherence-promoting substances, enzyme inhibitors and various trace elements, a-MEM and double antibodies (100 U). / mL penicillin + 100 ug / mL streptomycin);
[0028]Growth factors include PDGF-AB: 21.45-60.49ng / mL, PDGF-BB: 13.57-32.63ng / mL, FGF: 3.76-14.5ng / mL, EGF: 3-6.12ng / mL, TGF-β1: 7.45-13.84 ng / mL, bFGF: 40.71-97.89 ng / mL;
[0029] Hormones including insulin: 0.1-10 μg / ml;
[0030] Proteins include globulin: 1.8-2g / dL, albumin: 3.0-3.6g / dL;
[0031] Vitamin amino acids include choline chloride 0.5-0.8 mg / dL, calcium D-pantothenate 1-3 mg / L, folic acid 0.1-3 mg / L, niacinamide 0.2-0.3 mg / dL, pyridoxine hydrochloride 0.01-0.03 mg / L dL, Riboflavin 0.1-0.5 mg / L, Vitamin B35-10 mg / dL, Vitamin B6 0.1-1 mg / dL, Vitamin B12 0.1-0.8 mg / L, Inositol 1-3 mg / dL, Glycine 2-4 mg / d...
Example Embodiment
[0036] Embodiment 2 A kind of serum-free culture medium
[0037] (TBD-2) A serum-free medium for dental pulp stem cells including 10% serum-free supplement (purchased from Tianjin Haoyang Huake Biotechnology Co., Ltd.), 90% basal medium (purchased from Tianjin Haoyang Huake Biotechnology Co., Ltd. company) and double antibody (100 U / mL penicillin + 100 ug / mL streptomycin)
[0038] Serum-free supplements include recombinant human albumin, recombinant human transferrin, recombinant human insulin, rhEGF, rhbFGF, rhPDGF-BB, rhSCF, and fibronectin
[0039] Basal medium includes amino acids, vitamins, inorganic salts, sodium selenite, glucose, phenol red and sodium pyruvate
[0040] Amino acids include glycine, L-alanine, L-arginine hydrochloride, L-asparagine, L-aspartic acid, L-cystine hydrochloride, L-glutamic acid, L-group Amino acid hydrochloride, L-isoleucine, L-leucine, L-lysine hydrochloride, L-methionine, L-phenylalanine, L-proline, L-serine, L- -Threonine, L-tryptophan,...
Example Embodiment
[0043] Embodiment 3 A kind of serum-free culture medium
[0044] (YC-1) A serum-free medium for dental pulp stem cells including serum-free medium (purchased from Youkang Hengye Biotechnology Co., Ltd.) and dual antibodies (100 U / mL penicillin + 100 ug / mL streptomycin)
[0045] Serum-free medium including amino acids: glycine, L-alanine, L-arginine hydrochloride, L-asparagine, L-aspartic acid, L-cysteine hydrochloride, L-glutamate Amino acid, L-glutamine, L-histidine hydrochloride, L-isoleucine, L-leucine, L-lysine hydrochloride, L-methionine, L-phenylalanine , L-proline, L-serine, L-threonine, L-tryptophan, L-tyrosine and L-valine. Vitamins include choline chloride, calcium D-pantothenate, folic acid, niacinamide, pyridoxine hydrochloride, riboflavin, thiamine hydrochloride, vitamin B12, and inositol. Inorganic salts include: calcium chloride, magnesium chloride, magnesium sulfate (7H2O), potassium chloride, sodium chloride, disodium hydrogen phosphate (7H2O), sodium dihy...
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