Active ingredients of camptosorus sibiricus, and extraction method and use of same

A technology of active ingredients and extraction methods, which is applied in the field of production and processing of active ingredient preparations of fern fern, to achieve the effect of definite curative effect, simple preparation method and scavenging free radicals

Active Publication Date: 2009-09-23
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the extraction of its active ingredients and the production and processing of its active ingredients into preparations

Method used

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  • Active ingredients of camptosorus sibiricus, and extraction method and use of same
  • Active ingredients of camptosorus sibiricus, and extraction method and use of same
  • Active ingredients of camptosorus sibiricus, and extraction method and use of same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] According to the active ingredient preparation method 1

[0027] (1) Take 10kg of dry whole fern, remove impurities and dust

[0028] (2) Add medicinal materials and 18 times the amount of water in the traditional Chinese medicine extraction tank, heat and boil, and extract twice, each time for 2 hours.

[0029] (3) The two extracts were combined, concentrated to 20 L, and left to stand at 4° C. for 24 hours until the precipitation was complete, and the precipitate was removed.

[0030] (4) Take the supernatant and add ethanol, adjust the alcohol concentration to 50%, let stand at 10°C for 24 hours at low temperature until the precipitation is complete, filter again, and recover the ethanol from the filtrate until it has no alcohol smell.

[0031] (5) The supernatant is uploaded to the non-polar macroporous resin column, and water (8V R ), 20% ethanol (4V R ), 60% ethanol (4V R ), 90% ethanol (4V R ) gradient elution, the washings obtained were discarded, and the 2...

Embodiment 2

[0033] According to the active ingredient preparation method 2

[0034] (1) Take 5kg of dry whole fern, remove impurities and dust

[0035] (2) Add medicinal materials and 20 times the amount of 70% ethanol in the traditional Chinese medicine extraction tank, heat and reflux extraction three times, respectively for 2 hours, 1.5 hours, and 1 hour.

[0036] (3) The three extracts were combined, concentrated to 8 L, and left to stand at 4° C. for 12 hours until the precipitation was complete, and the precipitate was removed.

[0037] (5) The supernatant is uploaded to the non-polar macroporous resin column, and water (8V R ), 30% ethanol (4V R ), 70% ethanol (4V R ), 95% ethanol (4V R ) gradient elution, get the washings and discard the 30% ethanol eluate, 70% ethanol eluate, and 95% ethanol eluate and mix according to the ratio of 1:1:1 of the equivalent crude drug amount to obtain the effective extract of Fern fern. Wherein the total flavonoid content is 33%, the total sap...

Embodiment 3

[0039] According to the active ingredient preparation method 3

[0040] (1) Take 6kg of dry whole fern, remove impurities and dust

[0041] (2) Add medicinal materials and 25 times the amount of 75% ethanol in the traditional Chinese medicine extraction tank, heat and reflux extraction three times, respectively for 2 hours, 1.5 hours, and 1 hour.

[0042] (3) The three extracts were combined, concentrated to 10 L, and left to stand at 4° C. for 20 hours until the precipitation was complete, and the precipitate was removed.

[0043] (4) The supernatant was extracted four times with an equal volume of petroleum ether, ethyl acetate and n-butanol. Obtain 320 grams of petroleum ether extract, 60 grams of ethyl acetate extract, and 180 grams of n-butanol extract. Lysozyme was recovered, and the obtained n-butanol extract was further purified by macroporous resin, and eluted with water and 95% ethanol respectively, and lysozyme was recovered under reduced pressure to obtain 140 g ...

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Abstract

The invention belongs to the technical field of medicaments and provides active ingredients of Chinese herbal medicine camptosorus sibiricus and an extraction method and use of the same. The extraction method comprises a water extraction method, an alcohol extraction method, a macroporous resin purification method and a solvent extraction method. The main active ingredients of an extract obtained by the method are total flavonoids, total momordica saponins and total organic acids, wherein the content of the total flavonoids is 1 to 35 percent, the content of the total momordica saponins is 1 to 25 percent and the content of the total organic acids is 1 to 3 percent. The invention also provides a method for preparing the camptosorus sibiricus into a proper formulation directly or by mixing with solid or liquid commonly used for preparations of the type in pharmaceutics. The active ingredients of the camptosorus sibiricus and the preparation thereof have functions of treating thromboangiitis obliterans, nephritis, traumatism and bleeding, metrorrhagia, neurodermitis, hemiplegia caused by cerebral embolism and diabetes-related complications. The extraction method is simple, easy to implement and low in cost. And the extract obtained is free from toxic and side effects.

Description

Technical field: [0001] The invention belongs to the technical field of medicine, and relates to an extraction method and application of active ingredients of Chinese herbal medicine Pteris sublime, and a production and processing method of active ingredient preparations of Pteris sublime. Background technique: [0002] Overmountain fern (Camptosorus sibiricus Rupr.), also known as Maddengrass, Huanyanggrass, and Bridgegrass, is a perennial herb belonging to the Asplenaceae family. There are two kinds of this kind in the world, and one kind is made in China. Guoshan fern is a traditional herbal medicine. It is used in the folk to treat traumatic bleeding, uterine bleeding, thromboangiitis obliterans, nephritis, neurodermatitis, hemiplegia caused by cerebral embolism and complications of diabetes, etc. It has definite curative effect. Experiments have shown that the herbal medicine has very little toxicity and has no obvious effect on blood pressure, and long-term administra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/00C07D311/30C07H17/07C07C65/03C07C59/52C07H15/203A61K36/11A61P7/02
Inventor 李宁李铣张鹏马忠俊
Owner SHENYANG PHARMA UNIVERSITY
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