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Hair follicle reconstruction method based on hair follicle stem cells and fibroblast

A technology of hair follicle stem cells and fibroblasts, applied in medical science, prostheses, etc., can solve the problems of limiting the application of epidermal stem cells, inability to form epidermis, and easy loss of hair follicle ability, so as to achieve strong practical application prospects and scientific research potential, and avoid bacteria Pollution, reconstruction efficiency is high

Inactive Publication Date: 2012-02-01
INST OF ZOOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research on hair follicle stem cells in my country is still in its infancy, and there are few researchers on hair follicle stem cells. Although some people have tried to reconstruct hair follicles, such as injecting hair follicle bulge cells and dermal papilla cells into nude mice, or injecting keratinocytes and dermal papilla cells are cultured with scaffold materials and then transplanted to the skin of nude mice. It is possible to obtain structures similar to hair follicles, but there are various shortcomings. For example, the hair follicle bulge cells and dermal papilla cells are injected subcutaneously, although It can form hair follicle analogs, but the hair shaft cannot grow out of the skin surface, nor can it form the epidermis; and dermal papilla cells grow very slowly in vitro, and it is difficult to obtain sufficient cells for transplantation; in addition, dermal papilla cells are easy to grow in vitro for a long time Loss of ability to induce hair follicle formation
These limit the application of epidermal stem cells in skin and hair follicle reconstruction
It can be seen that a reliable hair follicle reconstruction method has not been established, and this technical gap greatly restricts the development and application of hair follicle stem cell research

Method used

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  • Hair follicle reconstruction method based on hair follicle stem cells and fibroblast
  • Hair follicle reconstruction method based on hair follicle stem cells and fibroblast
  • Hair follicle reconstruction method based on hair follicle stem cells and fibroblast

Examples

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Effect test

Embodiment 1

[0035] Example 1 Preparation of adult rat hair follicle stem cells

[0036] In this example, taking adult rat vibrissae hair follicle stem cells as an example, the method for preparing seed cells for hair follicle reconstruction of the present invention is described in detail, as follows. The feeding and use of experimental animals were carried out in accordance with the regulations of the Animal and Medical Ethics Committee of the Institute of Zoology, Chinese Academy of Sciences.

[0037] 1. Microdissection of rat vibrissae hair follicles

[0038] Take a small piece of adult Wistar rat (Institute of Zoology, Chinese Academy of Sciences) tentacles skin, disinfect with 75% alcohol for 3-5 minutes; under the stereoscope, use tweezers to clamp the isthmus of the tentacles hair follicles near the epidermis, and apply force toward the dermis Tear out the hair follicles; fix the hair follicles with a 29G needle, carefully scratch the dermal sheath with a scalpel, and peel off th...

Embodiment 2

[0046] Example 2 Hair follicle reconstruction based on adult rat hair follicle stem cells and newborn rat fibroblasts

[0047] 1. Preparation of fibroblasts

[0048] Pre-incubate the cell culture dish with fetal bovine serum in a 37°C incubator for 2 hours; kill the newborn rats (Experimental Animal Center, Institute of Zoology, Chinese Academy of Sciences), disinfect with 75% alcohol for 3-5 minutes, and then rinse with normal saline More than 3 times; in the ultra-clean workbench, cut the back skin with sterilized scissors; scrape off the subcutaneous fat with a scalpel; cut the skin into small pieces of 0.5cm×0.5cm; blot the serum in the culture dish, Spread the small pieces of skin evenly in the dish with the epidermis facing up. After incubating in a 37°C incubator for 2 hours, add DMEM medium containing 10% fetal bovine serum for culture; replace the culture medium every 2 days, and cultivate until needed The amount of cells was spared.

[0049] 2. Labeling of hair fol...

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Abstract

The invention provides a hair follicle reconstruction method based on hair follicle stem cells and fibroblast. The method provided by the invention comprises the steps of: (1) culturing and amplifying the hair follicle stem cells; (2) culturing and amplifying the fibroblast; and (3) mixing the amplified hair follicle stem cells and the amplified fibroblast, and inoculating a mixture to a position to be subjected to hair follicle reconstruction. Because the hair follicle stem cells can be passed massively in a long term and the dermis fibroblast is convenient to obtain, the method provided by the invention has very strong actual application prospect and scientific and research potential, is an important method for reconstructing skin and hair follicles in treating large-area skin injury (such burns, scalds and the like), and is also a first choice to testify the hair follicle stem cells in scientific researches.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a hair follicle reconstruction method based on hair follicle stem cells and fibroblasts. Background technique [0002] Hair follicles are appendages of mammalian skin and have physiological functions such as temperature regulation, sensation, secretion and excretion. In addition, through camouflage, it can help mammals avoid natural enemies; through body surface decoration, it can transmit social information and play a role in reproductive behavior; Great mental anguish. Existing knowledge shows that hair follicles play an important role not only in beauty, but also in wound healing of skin injuries (such as burns or scalds, etc.) and skin tumorigenesis. [0003] Hair follicles are one of the few micro-organs in mammals that maintain periodic morphological changes after adulthood. In recent years, scientists have received extensive attention and research, but there are ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/60
Inventor 段恩奎张守兵胡慧敏张会闪刘爽
Owner INST OF ZOOLOGY CHINESE ACAD OF SCI
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