Coating layer material suitable for adhesion and survival of neurons and preparation method thereof

A technology for coating materials and neurons, which is applied in biochemical equipment and methods, coatings, nervous system cells, etc., and can solve problems that are not related to medical use

Active Publication Date: 2019-04-02
JILIN UNIV
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  • Application Information

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  • Coating layer material suitable for adhesion and survival of neurons and preparation method thereof
  • Coating layer material suitable for adhesion and survival of neurons and preparation method thereof
  • Coating layer material suitable for adhesion and survival of neurons and preparation method thereof

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Embodiment

[0040] The experimental conditions and parameters for preparing a coating suitable for neuron adhesion and survival are as follows:

[0041] 1) Graphene oxide colloids were prepared by the modified Humners method. The raw materials are 1.5g sodium nitrate, 2g natural flake graphene, 9g potassium permanganate, 69ml concentrated sulfuric acid; use 10% hydrochloric acid and deionized water as washing liquid respectively, and centrifugally clean until the pH value of the supernatant is close to neutral. Obtain graphene oxide colloid;

[0042] 2) Take a certain amount of graphene oxide colloid and add water to dilute to a concentration of 0.5 mg / ml, and perform ultrasonic dispersion treatment on the re-diluted solution, and the ultrasonic time is 20 minutes;

[0043] 3) Dilute cytarabine with a small amount of deionized water, and the prepared concentration is 0.1mg / ml;

[0044] 4) The mixed solution that the ratio of graphene oxide and cytarabine is 4:1 is drop-coated on the gla...

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Abstract

The invention relates to a coating layer material suitable for adhesion and survival of neurons and a preparation method thereof. Generally speaking, the invention relates to a method for a graphene-based drug-loaded composite coating layer, wherein the method comprises the steps: the characteristics of a graphene-based material which has tissue inductivity and can be applied in the field of biomedical tissue engineering are used, a graphene oxide colloid is prepared by an improved Hummers method, then a diluted graphene oxide solution is evenly mixed with a cytosine arabinoside solution, andfinally, the graphene-based drug-loaded composite coating layer is prepared by vacuum drying treatment. The product prepared by the method can significantly promote the adhesion and survival of the neuron cells. The method provided by the invention is simple and easy to operate, low in cost, good in consistency and easy to transform. A prepared graphene-based film has broad application prospects and practical value in the field of tissue engineering.

Description

Technical field: [0001] The present invention relates to a coating material suitable for neuron adhesion and survival and a preparation method. Generally speaking, the present invention utilizes the characteristics that graphene-based materials have tissue induction and can be applied to the field of biomedical tissue engineering. The graphene oxide colloid is prepared by the improved Hummers method, and then the diluted graphene oxide solution is uniformly mixed with the cytarabine solution, and finally a graphene-based drug-loaded composite coating is prepared through vacuum drying. Background technique: [0002] Life and regenerative engineering, including the ability of humans to manipulate the nervous system, biological cells, tissues and organs in the future, is considered to be one of the disciplines involved in the sixth technological revolution in science and industry. As an important technology of the fourth industrial revolution, regenerative technology has become...

Claims

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Application Information

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IPC IPC(8): C12N5/0793C09D1/00C09D7/63
CPCC09D7/63C12N5/0619C09D1/00C12N2533/20C12N2537/00
Inventor 王欣王苹鞠婷婷刘洋王赞周余来
Owner JILIN UNIV
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