A preparation method of hydrogel composite biological patch for repairing abdominal wall defect

A biological patch and hydrogel technology, applied in the field of biomedical tissue engineering, can solve the problem of difficult vascularization and oxygen, nutrient exchange and metabolite discharge, difficult to grow host cells and new tissues, and poor mechanical properties. It can help the growth of cells and tissues, promote the neogenesis and vascularization of abdominal wall tissue, and control the mechanical strength and degradation rate.

Active Publication Date: 2021-11-05
SHANGHAI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the following problems still exist: 1. When a single decellularized tissue is used for abdominal wall repair, its mechanical properties are poor and it is difficult to support the growth of cells and tissues; 2. After the decellularized tissue is implanted in the body, its degradation rate is too fast, and the new tissue does not Reconstruction is complete, resulting in a susceptibility to hernia recurrence at the injury site
3. When the decellularized tissue is directly used for surgical repair, its material is too dense, and it is difficult to vascularize, exchange oxygen and nutrients, and excrete metabolites
In addition, it is difficult for host cells and new tissues to grow into the material, which is not conducive to defect repair

Method used

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  • A preparation method of hydrogel composite biological patch for repairing abdominal wall defect
  • A preparation method of hydrogel composite biological patch for repairing abdominal wall defect
  • A preparation method of hydrogel composite biological patch for repairing abdominal wall defect

Examples

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Embodiment example 1

[0022] see Figure 1 to Figure 3 , the preparation method of the hydrogel composite biological patch for repairing abdominal wall defects, the specific preparation steps are as follows:

[0023] a. Design of the mold structure: the mold consists of two parts: the mold base and the jacket. There are evenly distributed vertical cylinders on the base. The distance between adjacent cylinders is 500-800 microns, and the diameter of the cylinders is 500±5 microns. The shape of the mold is and size can be designed according to demand;

[0024] b. Preparation of pouring solution: Weigh a set amount of gelatin in a beaker, add deionized water, stir and dissolve in water at 60 degrees Celsius, and make a gelatin solution with a concentration of 15±1%; then weigh the biological crosslinking agent microorganism The mass ratio of transglutaminase to gelatin is 1:10±0.1; wait for the temperature of the gelatin solution to drop to room temperature, add the cross-linking agent and stir rapi...

Embodiment example 2

[0027] In this example, refer to figure 1 , figure 2 , image 3 , the preparation method of the hydrogel composite biological patch for repairing abdominal wall defects, using porcine dermis decellularized matrix powder and gelatin, through the method of mold casting and enzymatic cross-linking to prepare a hydrogel containing pre-vascularized channels Glue composite biological patch scaffold, the specific preparation steps are as follows:

[0028]a. Preparation of pouring solution: place the beaker on the electronic scale, first adjust to zero and then weigh the set amount of gelatin in the beaker, then add deionized water to the beaker to make the gelatin concentration 15%, and then put Its beaker is placed in the water bath of 60 ℃ and fully stirred, and its gelatin is all dissolved; Then take the biological cross-linking agent microbial transglutaminase of the gelatin of setting amount again, the weight ratio of its amount and gelatin is 1: 10. Put the gelatin solution...

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Abstract

The invention relates to the technical field of biomedical tissue engineering, and discloses a method for preparing a hydrogel composite biological patch for repairing abdominal wall defects. In the preparation method, pig dermis de-extracellular matrix powder and gelatin are used as biomaterials, and a composite hydrogel biopatch is prepared by mold casting and enzymatic cross-linking forming techniques. The present invention mixes the biomaterial porcine dermis de-extracellular matrix powder with gelatin to prepare the patch, not only the hydrogel patch exhibits excellent biocompatibility, but also enhances the biomechanical properties of the hydrogel patch, solving the problem There are deficiencies with single material patches. At the same time, the preparation process is simple and the required materials are easy to obtain. The composite biological patch scaffold prepared by the present invention contains pre-vascularized channels, which is helpful for the exchange of oxygen and nutrients and the discharge of metabolic waste, and accelerates the vascularization of tissues; in addition, the through-channel structure helps cells and tissues in the scaffold The ingrowth can accelerate the reconstruction of abdominal wall tissue, help to solve the existing technical bottleneck of biological mesh, and effectively improve the therapeutic effect of abdominal wall defect repair.

Description

technical field [0001] The invention relates to the technical field of biomedical tissue engineering, in particular to a method for preparing a hydrogel composite biological patch for repairing abdominal wall defects. Background technique [0002] In today's clinical surgery, abdominal wall defect is a common disease. Abdominal wall hernia, severe war trauma on the abdominal wall, accidents, enlarged resection of abdominal wall tumors, etc. can all cause the formation of abdominal wall defects. The traditional surgical treatment is mainly to forcibly suture the muscles or ligaments around the abdominal wall defect. After the operation, the patient suffers from obvious pain, long recovery time and high recurrence rate. The emergence of artificial mesh has made a breakthrough in the treatment of abdominal wall. The abdominal wall defect is sutured through the intra-abdominal mesh implantation to reduce the tension of the abdominal wall and speed up the wound repair of the pat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/44A61L27/52A61L27/58
CPCA61L27/362A61L27/44A61L27/52A61L27/58A61L2430/00C08L89/00
Inventor 胡庆夕刘随红张海光唐海沪陈箫
Owner SHANGHAI UNIV
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