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Bionic cell-containing large bone cartilage biological scaffold and preparation method thereof

A bio-scaffold, osteochondral technology, applied in the field of biomedical tissue engineering, can solve the problems of surrounding tissue fit, single, unable to meet the functional requirements of natural osteochondral cartilage surface structure requirements, etc., to speed up repair and promote integrated repair and reconstruction. Effect

Active Publication Date: 2022-03-04
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current osteochondral scaffold structure is relatively simple and single, which cannot meet the cartilage surface structure requirements of natural osteochondral and the functional requirements of large osteochondral tissue, and cannot fit with the surrounding tissue after implantation, and there is support for large osteochondral tissue. , vascular network channels and the interaction of cell-induced differentiation

Method used

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  • Bionic cell-containing large bone cartilage biological scaffold and preparation method thereof
  • Bionic cell-containing large bone cartilage biological scaffold and preparation method thereof

Examples

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preparation example Construction

[0031] The invention provides a method for preparing a bionic cell-containing large bone and cartilage bioscaffold, comprising the following steps:

[0032] The subchondral bone material containing bone marrow mesenchymal stem cells is subjected to the first extrusion 3D printing, and the sacrificial material containing human umbilical vein endothelial cells is used in the formed subchondral bone scaffold structure for the second extrusion 3D printing, alternately Performing the first extrusion 3D printing and the second extrusion 3D printing to obtain a subchondral bone layer containing a prefabricated vascularized network structure;

[0033] Using electrospun materials containing bone marrow mesenchymal stem cells, electrospinning is performed on the subchondral bone layer, and a cell-containing nanofiber isolation membrane is formed on the upper surface of the subchondral bone layer;

[0034] Continue to use the electrospun material containing bone marrow mesenchymal stem c...

Embodiment 1

[0077] according to figure 1 Shown flow process carries out the scheme of embodiment 1:

[0078] a. Preparation of bone stem cell bioink: using PBS buffer as solvent, gelatin (10%), sodium alginate (5%), bone marrow mesenchymal stem cells (5×10 6 cells / g) were mixed and prepared, and 5% of BMP-2 protein was added (w / w, relative to the total mass of bone stem cell bioink); 10 mL of the prepared bone stem cell bioink was added to a medical syringe, and the syringe Installed on print head one;

[0079] Preparation of cell-containing sacrificial material: using PBS buffer as solvent, collagen (5%, w / v), PVA (10%, w / v) and human umbilical vein endothelial cells (5×10 6 cells / g) were mixed and prepared; then 10 mL of the prepared cell-containing sacrificial material was added into a medical syringe, and installed on the printing nozzle 2;

[0080] b. Preparation of electrospun materials containing stem cells: PBS buffer as solvent, PEO (15%), sodium alginate (5%) and human umbili...

Embodiment 2

[0088] The difference between this embodiment and embodiment 1 is only:

[0089] Bone stem cell bioink, the solvent is HBSS solution (Hank's balanced salt solution), using methacrylated gelatin (12% w / v), hydroxyapatite (5% w / w) and bone marrow mesenchymal stem cells (5 ×10 6 cells / g) were mixed and prepared, and 0.2% (w / w, relative to the total mass of the stem cell bioink) was added with LAP photoinitiator;

[0090] The solvent of chondrocyte stem cell bioink is HBSS solution (Hank's balanced salt solution), using methacrylated gelatin (10% w / v), hyaluronic acid (10% w / v) and bone marrow mesenchymal stem cells (5×10 6 cells / g) were mixed and prepared, and 0.2% (w / w, relative to the total mass of cartilage stem cell bioink) was added with LAP photoinitiator;

[0091] Place the prepared bulk osteochondral scaffold under blue light with a wavelength of 405nm for cross-linking;

[0092] Other steps are with embodiment 1.

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Abstract

The invention provides a bionic cell-containing large bone cartilage biological scaffold and a preparation method thereof, and belongs to the technical field of biomedical tissue engineering. According to the invention, a composite process including a cell extrusion 3D printing technology, a cell electrostatic spinning technology, a cell electrohydrodynamic direct writing technology and a sacrificial material method is adopted, so that not only can the formation of an integrated osteochondral scaffold with a bionic human osteochondral tissue structure be realized, but also the preparation of a cell-containing osteochondral scaffold can be realized; a solution is provided for construction of a large osteochondral biological scaffold, and integrated repair and reconstruction of osteochondral tissues are promoted.

Description

technical field [0001] The invention relates to the technical field of biomedical tissue engineering, in particular to a bionic cell-containing large bone cartilage biological scaffold and a preparation method thereof. Background technique [0002] Due to trauma, accidents, obesity, strain, tumors, congenital abnormalities and deformities of joints, and senile degenerative osteoarthropathy, the number of patients with articular osteochondral defects is increasing, and it has become one of the main causes of physical disabilities. Among them, articular cartilage damage can easily lead to combined lesions of the subchondral bone, making it very difficult for the damaged articular cartilage tissue to repair itself. Therefore, it has become one of the world's most urgent orthopedic problems to be solved in clinical orthopedics. At present, implants such as autologous or allogeneic tissues and artificial scaffolds are mainly used for clinical repair. Among them, the source of aut...

Claims

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Application Information

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IPC IPC(8): A61L27/38A61L27/12A61L27/16A61L27/18A61L27/20A61L27/22A61L27/24A61L27/56B33Y70/10
CPCA61L27/3834A61L27/3852A61L27/24A61L27/222A61L27/20A61L27/12A61L27/16A61L27/18A61L27/56B33Y70/10A61L2430/06A61L2400/12
Inventor 胡庆夕刘随红张海光
Owner SHANGHAI UNIV
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