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Polypeptide molecular derivative with mineralizing function for biomedicine tissue engineering and application thereof

A tissue engineering and biomedical technology, applied in the field of biomedical materials, can solve the problem of lack of biological materials, and achieve the effect of good biological activity

Active Publication Date: 2019-08-27
NANJING JIUZHENWEI MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003]There is a lack of biomaterials with efficient mineralization function for cementum regeneration in the prior art

Method used

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  • Polypeptide molecular derivative with mineralizing function for biomedicine tissue engineering and application thereof
  • Polypeptide molecular derivative with mineralizing function for biomedicine tissue engineering and application thereof
  • Polypeptide molecular derivative with mineralizing function for biomedicine tissue engineering and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 The biological activity of polypeptide derivative CEMP1 biomimetic oligopeptide

[0033] Peptide derivative CEMP1 biomimetic oligopeptide, the structural formula is:

[0034] C 16 h 31 0-Asn-Asn-Cys-Cys-Cys-Cys-Arg-Arg-Glu-Ser(p)-NH 2 , wherein Ser(p) represents phosphorylated serine.

[0035] 1. Induce self-assembly to obtain nano-hydrogels, which can be used as scaffold materials for bone and tooth tissue engineering, or applied to 3D in situ culture of cells.

[0036] A: Take 2mg of peptide derivative CEMP1 biomimetic oligopeptide powder and add 100ul of dimethylsulfoxide (DMSO) to dissolve it completely, then add 1900ul of deionized water to make a 1mg / ml peptide solution.

[0037] B: Take 200ul of the polypeptide derivative solution with a concentration of 1mg / ml obtained in step A, add 1M calcium chloride solution, ultrasonically oscillate, mix thoroughly, self-assemble at room temperature for 2 hours, and obtain a semi-solid hydrogel, that is, self-...

Embodiment 2

[0059] According to the method in Example 1, the polypeptide derivative 2-96 is tested in four aspects as follows:

[0060] 1. Induce self-assembly to obtain nano-hydrogels, which can be used as scaffold materials for bone and tooth tissue engineering, or applied to 3D in situ culture of cells.

[0061] Results: The polypeptide derivatives can self-assemble to form nanofiber structures, and the nanofibers interweave each other to form a porous network structure. The diameter of this nanofiber is between 40-60nm; the length can reach micron level. Therefore, the peptide derivative CEMP1 biomimetic active molecule can self-assemble under the induction of calcium chloride to form a 3D nano-hydrogel, which can be applied to bone and tooth tissue engineering scaffold materials, or to 3D in situ culture of cells.

[0062] 2. Self-assembled polypeptide derivatives induce the growth of apatite crystals to obtain new bone-like repair materials.

[0063] Results: Granular mineralizati...

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Abstract

The invention provides a polypeptide molecular derivative with a mineralizing function for biomedicine tissue engineering and application thereof, and belongs to the technical field of biomedical materials. The polypeptide molecular derivative has a molecular structure general formula of X-X-Cys-Cys-Cys-Cys-Y-Y-Z-W-NH2; wherein X is Asn or glucoylated asparagine; Y is Arg or Lys; Z is Glu or Asp;and W is phosphorylated serine or phosphorylated threonine. The polypeptide molecular derivative provided by the invention can induce the oriented growth of apatite crystals to form a biological material with a structure similar to an organism sclerous tissues microstructure; can be automatically assembled to form 3D (three-dimensional) nanometer hydrogel under the induction of calcium chloride, so as to be applied to a scaffold material for bone tissue engineering or to 3D in-situ cultivation of cells; can be compounded with collagenous fiber to induce the mineralization of the collagenous fiber, so as to be applied to study on bone tissue regeneration, as well as to induce the mineralizing regeneration and repair of cementum and dentin.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a polypeptide molecular derivative with mineralization function. Background technique [0002] Once the hard tissue of the body is damaged, it is difficult to achieve self-healing repair, so the regeneration and repair of hard tissue is one of the important research topics in clinical medicine and stomatology. One of the keys to achieve human hard tissue regeneration is to induce the formation of calcium phosphate by growth factors or their biomimetic analogs. The regeneration of periodontal tissue in stomatology is more complicated. One of the keys to rebuilding a functional periodontal complex is to attach the fibrous connective tissue of the periodontal ligament to the mineralized layer on the surface of the cementum and alveolar bone. Among them, cementum is an avascular mineralized hard tissue attached to the surface of the root, and the fibrous co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06A61L27/52A61L27/22A61L27/02C12N5/00
CPCC07K7/06C07K19/00A61L27/52A61L27/227A61L27/025C12N5/0062A61L2430/02A61L2430/12C12N2533/50C08L89/00
Inventor 孙卫斌王晴晴苗雷英李全利
Owner NANJING JIUZHENWEI MEDICAL TECH CO LTD
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