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Novel bionic adhesive material, and preparation method and application thereof

An adhesive material and a new type of technology, applied in the preparation method of peptides, chemical instruments and methods, preparation of animal glue or gelatin, etc., can solve problems such as uncontrollable, limited application, weak adhesion strength, etc., to ensure consistency , improve work efficiency, and take effect quickly

Inactive Publication Date: 2020-12-25
南京大学深圳研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the adhesion strength of a single dopa molecule is weak, and most of the dopa adhesion is permanent and uncontrollable, which limits the application in cell capture

Method used

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  • Novel bionic adhesive material, and preparation method and application thereof
  • Novel bionic adhesive material, and preparation method and application thereof
  • Novel bionic adhesive material, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0031] A preparation method of a novel bionic adhesive material, the steps are as follows:

[0032] S1. Modification and protection of dopa molecule: firstly, in anhydrous CH 3 Add dihydroxyphenylalanine (Dopa) (2.0g, 10.1mmol) and TBDMS-Cl (4.6g, 30.5mmol) to CN (23.0mL); cool with ice-water bath for 10min, then add dropwise DBU (4.6mL, 30.2 mmol) more than 10min; after that, allow the reaction mixture to be ice-bathed for 4h, then react at room temperature for 20h; : Dichloromethane (from 1:9 gradually increased to 3:7) mixed solution is eluted to remove impurities, and the eluent is rotary evaporated to remove solvent to obtain white product Dopa (TBDMS) 2 -OH; Dopa (TBDMS) 2 -OH (8.62g, 31.3mmol) and 40mL 10% Na 2 CO 3 The solution was added to 113mL THF, and this process was carried out in an ice bath; FmocOSu (6.39g, 19.0mmol) was added after the mixture was stirred and reacted for 10 minutes, and after stirring and reacting for 4h under ice bath, the reaction was co...

Embodiment 1

[0039] Example 1 Morphological and mechanical characterization of the invention.

[0040] In the experiment, we used another two groups of peptide samples as controls to verify the principle of the excellent adhesion performance of 2-NapGFFDopa. 2-NapGFFY without dopa and 2-NapGAADopa containing dopa but unable to self-assemble, respectively, such as figure 2 As shown in A. Usually, the solid hydrogel can be obtained by quickly adding the polypeptide solution (DMSO dissolved) containing 2-NapGFFDopa or 2-NapGFFY into phosphate buffered saline (PBS, pH=7.4), and shaking and mixing evenly. product. However, 2-NapGAADopa is still in a liquid state because it cannot self-assemble to form a fiber network. It can also be seen from the atomic force microscope (AFM) scan that there are very obvious fiber network structures in the 2-NapGFFDopa and 2-NapGFFY samples, such as figure 2 Shown in B~D. Utilize the mechanical test that rheometer carries out also can find out, the stora...

Embodiment 2

[0041] Example 2 Test of the present invention on pH-responsive disassembly.

[0042] For pH-responsive disassembly, we use NaHCO 3 solution to adjust the pH range. Experiments showed that when the pH of the environment was adjusted from 7.4 to 8.4, the 2-NapGFFDopa hydrogel gradually changed from a solid state to a liquid state within 30 minutes, and the AFM scan also verified the disappearance of the fiber network structure. like image 3 As shown in A. The circular dichroism experiment also verified this point. The circular dichroism (CD) of the assembled 2-NapGFFDopa showed an obvious peak corresponding to the beta sheet structure at 215nm, while no obvious peak was observed in the decomposed peptide solution. signal, which is consistent with the AFM imaging results ( image 3 B). Rheological experiments showed that the storage modulus of 2-NapGFFDopa also began to be smaller than the loss modulus after the pH increase treatment, which also proved the fluidization of ...

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Abstract

The invention relates to a novel bionic adhesive material, and a preparation method and application thereof. The intelligent adhesive material is formed by self-assembly of dopa-containing polypeptidenanofibers; and the dopa-containing intelligent polypeptide nanofiber material has very excellent biological adhesion performance, can be widely applied to the fields of biomedicine, tissue engineering, intelligent soft materials and the like, and is suitable for capturing and releasing various biological cells so as to carry out research work of research and development of various biological materials and medicines.

Description

technical field [0001] The invention relates to a novel bionic adhesive material and its preparation method and application. Background technique [0002] Single-cell analysis and cell therapy have been widely explored and applied in disease diagnosis, cancer treatment, and new drug development. For example, single-cell analysis reveals the diversity of cellular behavior and provides richer data than commonly used cell assembly analysis; the detection and isolation of circulating tumor cells (CTCs) has important implications for cancer diagnosis and treatment. Therefore, the capture and release of cells is of great significance to the diagnosis and treatment of cells. Significant efforts have been made to develop efficient cell capture and release methods. In general, cell adhesion methods based on surface modifications that are sensitive to pH, temperature, voltage, and light have been developed as a control means to control cell adhesion on surfaces. For example, target...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09J189/00C09J11/06C07K5/103C07K1/107C07K1/06C07K1/04
CPCC07K5/1008C09J11/06C09J189/00C08K5/175Y02P20/55
Inventor 薛斌王天阔李英曹毅秦猛王炜
Owner 南京大学深圳研究院
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