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Method for inducing production of chondroblast from umbilical cord mesenchymal stem cells

A technology of mesenchymal stem cells and cartilage formation, applied in the field of stem cells, can solve the problems of small amount of cells, low success rate, and insufficient clinical use

Inactive Publication Date: 2018-11-06
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the traditional two-dimensional induction method has a low success rate, and the amount of cells obtained is small, which is not enough for clinical use.

Method used

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  • Method for inducing production of chondroblast from umbilical cord mesenchymal stem cells
  • Method for inducing production of chondroblast from umbilical cord mesenchymal stem cells
  • Method for inducing production of chondroblast from umbilical cord mesenchymal stem cells

Examples

Experimental program
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preparation example Construction

[0035] Wherein the preparation method of the gelatin particles is firstly dissolving the gelatin in water. The gelatin was used in an amount of 15% w / v. After dissolving in water, thermally decompose at 80°C. Add the oil phase in which emulsifier A is dissolved and stir. The component of emulsifier A is a mixture of liquid paraffin and olive oil. The volume ratio of gelatin volume to emulsifier A is preferably 3:1, stir at a speed of 500r / min, and then add emulsifier B for stirring. The composition of emulsifier B is a mixture of liquid paraffin and soybean oil. The amount of emulsifier B added is that the volume ratio of gelatin microspheres to emulsifier B is 1:3. Subsequently, the emulsion was rapidly cooled to below 5°C in an ice-water bath, and the stirring was continued at a speed of 250 r / min for a period of time to obtain an emulsion.

[0036] Then, add dehydrating agent A in an ice bath at -20°C to the emulsion for dehydration. The amount of dehydrating agent ad...

Embodiment 1

[0040] Embodiment 1, its formula of chondrogenic induction medium

[0041] Each 100ml contains:

[0042]

Embodiment 2

[0044] The umbilical cord mesenchymal stem cells of the second generation were obtained and passed on to the third generation. When the 3rd passage cells reached 80%-90% confluence ( figure 1 ), digested with 0.25% trypsin for 2 minutes, discarded the medium after centrifugation, resuspended and inoculated in a six-well plate that had been inoculated with gelatin microspheres for cultivation, and replaced it with the chondrogenic induction medium described in Example 1 the next day, Thereafter, the chondrogenic induction medium was replaced every 3 days for a total of 21 days of induction.

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Abstract

The invention belongs to the field of stem cells and discloses a method and medium for production of chondroblast from umbilical cord mesenchymal stem cells. The medium comprises a cytokine bFGF. Themethod utilizes gelatin microparticles as scaffold materials and a three-dimensional culture method to induce production of chondroblast from umbilical cord mesenchymal stem cells based on the medium.The method has good induction effects and a high success rate and can acquire a lot of cells.

Description

technical field [0001] The invention belongs to the field of stem cells, and in particular relates to a method for inducing chondrocytes from umbilical cord mesenchymal stem cells. Background technique [0002] In orthopedic work, articular cartilage damage from various causes is common. Osteochondral defects in joints are caused by trauma, surgical resection (such as tumors), infection, gout and degeneration, often manifested as intractable pain, joint movement disorders, and severe cases may lose joint function, becoming the main cause of disability and loss of working ability one of the reasons. Due to its own characteristics, such as no blood vessels, nerves and lymphatic drainage, the physiological characteristics of cartilage mainly rely on the synovial fluid in the joint cavity for nutrition, which determines its extremely limited regenerative ability and cannot repair itself. [0003] The current methods of treating cartilage damage have obvious defects, and tissue...

Claims

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Application Information

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IPC IPC(8): C12N5/077C12N5/0775
CPCC12N5/0655C12N2500/24C12N2500/38C12N2501/115C12N2501/15C12N2501/33C12N2506/1369
Inventor 陈海佳葛啸虎王一飞罗二梅王小燕
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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