Method for 3D cultivating and inducing stem cell of mesenchyme of bone marrow and chondroblast

A bone marrow mesenchymal and stem cell technology, applied in biochemical equipment and methods, tissue culture, fixed on/in organic carriers, etc., can solve the problem of increasing interference factors, affecting tissue and cell growth, and difficult to obtain correct Evaluation and other issues, to achieve the effect of facilitating delivery, good shape and function, and reducing the loss of cell multidirectional differentiation potential

Inactive Publication Date: 2005-01-12
EAST CHINA UNIV OF SCI & TECH
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Problems solved by technology

Several cartilage tissue engineering carrier materials commonly used at present, such as polylactic acid and polyglycolic acid, the acidic products after degradation in the body will affect the growth of tissues and cells, natural biological materials such as collagen, decalcified bone, fibrin gel, etc. , due to its own biological characteristics, increased interference factors, it is difficult to get a correct evaluation in the histological and biochemical identification of newborn cartilage (see Wei Xiaochun et al., The Third National Tissue Engineering Academic Conference and the First China International Tissue Engineering Conference Proceedings: 144-146)

Method used

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  • Method for 3D cultivating and inducing stem cell of mesenchyme of bone marrow and chondroblast
  • Method for 3D cultivating and inducing stem cell of mesenchyme of bone marrow and chondroblast

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Embodiment 1

[0031]Put 150 mg of Cytodex 3 microcarriers into the spinner bottle, infiltrate twice with phosphate buffer solution, 30 minutes each time, and finally soak in phosphate buffer solution, and put it into an autoclave to sterilize at 120°C for 30 minutes. After the spinner bottle is taken out, suck out the phosphate buffer in the ultra-clean workbench, add 30ml of α-MEM culture solution containing 10% by volume of fetal bovine serum, put the spinner bottle into the spinner machine and stir overnight. After the cultured bone marrow mesenchymal stem cell seed cells were digested with a digestion solution containing 0.02% ethylenediaminetetraacetic acid and 0.25% trypsin, they were transferred to a spinner bottle containing Cytodex 3, and the content of Cytodex 3 was adjusted to 3 mg / ml. Stirring speed is 50rpm, at 37°C, 5% CO 2 , Cultivation under saturated humidity, the cultivation time is 4 days, and half of the culture medium is replaced every day. After the cultivation is com...

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Abstract

A method for preparing chondroblast of bone marrow mesogalia stem cell with three dimensional cultivation and induction includes steps of placing bone marrow mesogalia stem cell into suspending culture device preset with culture medium and microcarrier for suspending cultivation; cloning abovesaid stem cells with agitation, adding induction culture medium of chondroblast in culture meidum containing harvested cytodex3 microcrrier and abovesaid stem cells gromn on it; and carrying out induction for chondroblast assembly from abovesaid stem cells.

Description

technical field [0001] The invention relates to a method for cultivating and inducing bone marrow mesenchymal stem cells into chondrocytes. Background technique [0002] Bone marrow is a complex tissue containing cells of various hematopoietic and non-hematopoietic lines. Bone marrow mesenchymal stem cells are a kind of non-hematopoietic stem cells that exist in the bone marrow. They are easily isolated from the bone marrow. They can not only proliferate rapidly in vitro, but also have multidirectional differentiation potential. It can generate various mesenchymal tissue cells, such as osteoblasts, chondrocytes, adipocytes, tenoblasts, bone marrow stromal cells and nerve cell-like cells. Based on the above two characteristics, bone marrow mesenchymal stem cells can undergo a variety of purposeful induction operations after in vitro culture and expansion, and then reinfuse them into the body for clinical treatment, such as treating fractures and cartilage tissue damage. Ther...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/08C12N11/02
Inventor 周燕谭文松蒋丹丹胡静波
Owner EAST CHINA UNIV OF SCI & TECH
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