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A kind of preparation method of platelet-rich plasma

A technology of platelet-rich plasma and plasma, which is applied in the direction of blood/immune system cells, animal cells, vertebrate cells, etc., can solve the problems of affecting the application effect, redness and swelling of the skin, limited platelet concentration multiple, etc., and achieves remarkable technical effects and high platelet concentration Improve and promote the effect of application value

Active Publication Date: 2018-11-20
HENAN JINTAI BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method, because it uses a one-step centrifugation method, due to the limitation of the centrifugation speed and time, is likely to cause the following problems: ① platelets cannot be centrifuged sufficiently, and the concentration of platelets in the collected PRP is limited. Specifically, the currently sold The concentration of concentrated platelets in the PRP separation tube is about 4 times; ② There is still a certain amount of red blood cells in the plasma, so the prepared PRP also contains a certain amount of red blood cells
[0005] In summary, the main technical defects related to the preparation of PRP in the prior art are: first, the concentration factor of platelets is limited; second, the prepared PRP contains a certain amount of red blood cells, which affects the application effect. The injection prepared by PRP is used for skin beautification. In the early stage of use, it will cause redness, swelling and dark purple of the skin at the injection site, which will affect the appearance, so further improvement is urgently needed.

Method used

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  • A kind of preparation method of platelet-rich plasma
  • A kind of preparation method of platelet-rich plasma
  • A kind of preparation method of platelet-rich plasma

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Embodiment 1~5

[0046] Furthermore, the inventors prepared PRP with the method for preparing platelet-rich plasma provided by the present invention, and the process is briefly introduced as follows.

[0047] The preparation method of platelet-rich plasma provided by the present invention comprises the following steps:

[0048] (1) Take blood collection tubes pre-filled with anticoagulant sodium citrate, collect 20 mL of venous blood from the elbow of volunteers under aseptic operation, and divide it into 15 mL centrifuge tubes;

[0049] (2) Slowly divide the blood collected in step (1) into 15mL centrifuge tubes containing lymphocyte separation liquid; in terms of volume ratio, lymphocyte separation liquid:blood=1:2;

[0050] (3) Centrifuge the whole blood for the first time: centrifuge for 8-10 minutes with a centrifugal force of 180-250 × g in a low-speed refrigerated centrifuge;

[0051] (4) Pasteur tube suction step (3) centrifuged upper 4 / 5 plasma into another new 15mL centrifuge tube; ...

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Abstract

The invention belongs to the technical field of blood products and particularly relates to a preparation method for platelet rich plasma (PRP). The method comprises a blood collection step, a lymphocyte separation medium treatment step, a first-time centrifugation step, a second-time centrifugation step and the like. According to the preparation method disclosed by the invention, on the basis of the existing PRP preparation technology, firstly, collected blood is treated by adopting a lymphocyte separation medium, and then, by optimizing a centrifugal force and time of centrifugation, the PRP concentration effect of the prepared PRP is further promoted, and the number of red blood cells is greatly reduced; the preparation method has better practical application value.

Description

technical field [0001] The invention belongs to the technical field of blood products, and in particular relates to a preparation method of platelet-rich plasma. Background technique [0002] Platelet-rich plasma, also referred to as PRP (Platelet-rich plasma), refers to autologous plasma rich in high-concentration growth factors produced by collecting your own venous blood and centrifuging to concentrate platelets and white blood cells. Regarding the application of PRP, the most popular one is the application of PRP in beauty. The injection made on the basis of PRP is injected into the skin through the superficial injection method of the dermis. A variety of autologous growth factors in PRP can penetrate into the entire skin tissue and adjust the skin. The full-thickness structure repairs aging and damaged skin tissue, so as to achieve the purpose of improving skin texture, tightening and lifting facial skin, reducing wrinkles and sunken scars, restoring skin youthfulness, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/078
Inventor 郝京生韩慧华张东丽李帅民
Owner HENAN JINTAI BIOTECH
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