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Environment-friendly preparation method for high-ester catechin tea polyphenol

A technology of tea polyphenols and catechins, which is applied in the field of green preparation of high-ester-type catechin-tea polyphenols, can solve the problem of low content of ester-type catechins, and achieve the effect of improving safety

Active Publication Date: 2011-11-16
TEA RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there have been many reports on the membrane treatment process and resin separation process for the preparation of tea polyphenols, but most of them have problems such as the use of more toxic organic solvents and metal ions, and the low content of ester-type catechins in tea polyphenol products.

Method used

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  • Environment-friendly preparation method for high-ester catechin tea polyphenol
  • Environment-friendly preparation method for high-ester catechin tea polyphenol

Examples

Experimental program
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Effect test

Embodiment 1

[0015] Weigh 50g of tea leaves, add 500ml of deionized water (80°C) to extract for 45 minutes, take out the extract, then add an equal volume of deionized water (80°C) to extract for 45 minutes, combine the extracts, pass through 200 mesh -400 Purpose Suction filtration with filter cloth, treatment with 0.2um ceramic membrane to obtain tea polyphenol microfiltrate, and then concentrated by RO membrane to obtain tea polyphenol membrane concentrate.

[0016] Fill the styrene-type medium polar resin with a diameter of 16mm and a length of In a 600mm chromatography column, the volume of the packed column bed is 80ml, and the resin is rinsed with 95% ethanol and water in sequence until the column bed is balanced. At room temperature, load the tea polyphenol film concentrate onto the resin column, rinse with 1.5BV of deionized water, 2BV of food grade ethanol: deionized water = 8:2 elution solvent, and the rinsing speed is 2BV / h, collect and combine the eluate, vacuum evaporation, ...

Embodiment 2

[0019] Weigh 50g of tea leaves, add 500ml of deionized water (80°C) to extract for 45 minutes, take out the extract, then add an equal volume of deionized water (80°C) to extract for 45 minutes, combine the extracts, pass through 200 mesh -400 Purpose Suction filtration with filter cloth, treatment with 0.2um ceramic membrane to obtain tea polyphenol microfiltrate, and then concentrated by RO membrane to obtain tea polyphenol membrane concentrate.

[0020] Fill the styrene-type medium-polarity resin with a diameter of 16 mm, In a chromatographic column with a length of 600 mm, the volume of the packed column bed is 80 ml, and the resin is sequentially washed with 95% ethanol and water until the column bed is balanced. At room temperature, load the tea polyphenol film concentrate onto the resin column, rinse with 1BV of deionized water, 2BV of food grade ethanol: deionized water = 8:2 mixed elution solvent, and the washing speed is 2BV / h, collect and combine the eluate, vacuum...

Embodiment 3

[0023] Weigh 250g of tea leaves, add 2000ml deionized water (90°C) to extract for 35min, take out the extract, then add an equal volume of deionized water (90°C) to extract for 35min, combine the extracts, pass through 200 mesh -400 Purpose Suction filtration with filter cloth, treatment with 0.2um ceramic membrane to obtain tea polyphenol microfiltrate, and then concentrated by RO membrane to obtain tea polyphenol membrane concentrate.

[0024] Fill the styrene-type medium-polarity resin with a diameter of 36mm and a length of In a 1000mm chromatographic column, the volume of the packed column bed is 700ml, and the resin is sequentially washed with 95% ethanol and water until the column bed is balanced. At room temperature, load the tea polyphenol film concentrate onto the resin column, rinse with 1.5BV of deionized water, 2BV of food grade ethanol: deionized water = 8:2 elution solvent, and the rinsing speed is 2BV / h, collect and combine the eluate, vacuum evaporation, conc...

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Abstract

At present, there are many reports on a membrane treatment process and a resin separation process for preparing tea polyphenol, but the membrane treatment process and the resin separation process have the problems of more toxic organic solvents and metal ions, low content of ester catechin in tea polyphenol products and the like. The invention provides an environment-friendly preparation method for high-ester catechin tea polyphenol. The environment-friendly and safe high-ester catechin tea polyphenol is prepared by the following preparation processes of: taking tea leaves as raw materials, leaching by using hot water, filtering by using a ceramic membrane, concentrating by using a reverse osmosis (RO) membrane, separating by using styrene neutral and polar resin, performing evaporation concentration under vacuum, drying and obtaining the high-ester catechin tea polyphenol. In the environment-friendly preparation method, a metal ion precipitant and toxic solvents such as ethyl acetate, chloroform, acetone, methylene dichloride and the like which are commonly used in the conventional process are not used in the integral process, the environment-friendly, safe and high-purity tea polyphenol product can be obtained, and the total content of catechin, particularly the content of the ester catechin with high bioactivity in the product can be improved effectively.

Description

[0001] technical field [0002] The invention relates to the extraction of main natural active ingredients in tea leaves, in particular to a green preparation method of high-ester catechin tea polyphenols. Background technique [0003] Tea polyphenols are the main natural active ingredients of tea, which have various physiological activities and pharmacological effects such as anti-oxidation, anti-radiation, anti-bacterial and anti-inflammatory, anti-cancer, and anti-cardiovascular and cerebrovascular diseases. Tea catechin is the main component of tea polyphenols, accounting for about 70%-80% of the total, and it is also the primary component of tea for health care. Tea catechins belong to flavanol compounds. The basic structure is α-phenylbenzopyran, which can be divided into ester catechins and non-ester catechins. The main difference in structure lies in the number and position of hydroxyl substitution. , Ester catechins mainly include epigallocatechin gallate (EGCG), e...

Claims

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Application Information

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IPC IPC(8): A61K36/82A61P39/06A61P35/00A61P31/04A61P31/12A61P17/16A61P3/10A61P3/06A61P9/10A61K127/00
Inventor 江和源张建勇江用文陈芃
Owner TEA RES INST CHINESE ACAD OF AGRI SCI
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