The invention relates to the technical field of spline shafts, in particular to a high-precision rectangular spline shaft manufacturing process. According to the high-precision spline shaft manufacturing process, first, materials are selected, namely, 40Cr materials (selected according to the requirement) are adopted; second, rough turning machining is conducted on the spline workblank; third, thermal refining is conducted, wherein the rigidity HB is 250-280, and spline shaft core strength is improved; fourth, digital controlled lathe semi-finish turning is conducted, wherein a reserved bearing block margin is 0.3-0.5mm; fifth, spline rolling is conducted, namely, a spline milling machine for a grinding hob is used for machining the spline width and the bottom diameter, the margin of 0.3-0.4mm is reserved; sixth, quenching (heating processing) is conducted, namely, high-frequency quenching is adopted at the spline position, the rigidity HR is 48-53, and the rigidity of the spline position is improved; seventh, external diameter grinding of a cylindrical grinding machine is conducted (drawing technical requirements are met as needed); eighth, spline grinding is conducted, namely, a molding gear grinding machine is adopted, and a grinding wheel is corrected; ninth, the spline size is detected. The high-precision rectangular spline shaft manufacturing process improves the precision of the spline shaft, and improves working efficiency.