Rapid rooting method in blueberry bottle

A blueberry, fast technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of cumbersome operation, long time required for rooting, etc., and achieve the effects of simple operation, high rooting rate, and wide application range.

Inactive Publication Date: 2017-06-09
BEIJING VOCATIONAL COLLEGE OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this patent pays more attention to the rooting management measures of blueberries in the stage of transplanting and domestication, that is, on the basis of rooting medium cultivation, rooting is carried out by spraying Shenggenbao again. longer time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: The test variety is O'Neill

[0022] 1) Spread a layer of filter paper in the culture bottle, then pour the prepared liquid culture medium (the formula is 1 / 2WPM+20g / L sucrose+0.3% activated carbon, pH is 5.8) into the culture bottle, then put the culture bottle and The Erlenmeyer flask containing IBA (concentration of IBA is 200mg / L) is sterilized at 121°C for 15 minutes for later use; 2) select blueberries that have been subcultured for about 35 days and have grown vigorously, and cut them into 3-4cm long blueberries. For the stem segment, put its base into the IBA solution and dip it at a medium speed for 10 seconds, and then put these stem segments vertically into the culture bottle; The cultivation was carried out under the cultivation conditions under d. After 30 days of cultivation under light, the rooting rate is 92%, the average root length is 2.7cm, and the survival rate after transplanting can reach 93%.

Embodiment 2

[0023] Embodiment 2: The test variety is O'Neill

[0024] (1) Spread a layer of filter paper in the culture bottle, then pour the prepared liquid culture medium (the formula is 1 / 2WPM+20g / L sucrose, also contains vitamin C, the addition amount is 0.001mmol / L, pH is 5.8) Put it into the culture bottle, then sterilize the culture bottle and the Erlenmeyer flask (IBA concentration is 200mg / L) with IBA at a high temperature of 121°C for 15min for later use; Cut the stems with a length of 3-4cm, dip their bases in IBA solution for 10 seconds at a medium speed, and then put these stems vertically into culture bottles; Under the culture conditions of 2000Lx and light duration of 14h / d, they were cultivated. After being cultivated under light for 30 days, the rooting rate can reach 90%, the average root length is 2.1cm, and the survival rate after transplanting can reach 92%.

Embodiment 3

[0025] Embodiment 3: The test variety is O'Neill

[0026] (1) Spread a layer of filter paper in the culture bottle, then pour the prepared liquid culture medium (the formula is 1 / 2WPM+20g / L sucrose, pH 5.8) into the culture bottle, and then put the culture bottle and IBA The Erlenmeyer flask (IBA concentration is 200mg / L) is sterilized under the high temperature of 121 ℃ for 15min for later use; 2) select the blueberry that grows vigorously after subculture for 35 days, cut it into the stem segment that is 3-4cm in length, and cut it into Put the base into the IBA solution and dip it at medium speed for 10 seconds, and then put these stems vertically into the culture bottle; 3) Culture in dark light for a week, and then under the culture conditions of light intensity of 2000Lx and light duration of 14h / d down to cultivate. After being cultivated under light for 30 days, the rooting rate is 87%, the average root length is 2.0cm, and the survival rate after transplanting can re...

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Abstract

The invention provides a rapid rooting method in a blueberry bottle, wherein the rooting device includes steps of 1), paving a layer of filter paper in a culture bottle, and pouring the prepared liquid culture medium in the bottle; then sterilizing the culture bottle and a triangular bottle filled with IBA under the temperature of 120-125 DEG C for 15-20 min for future use; 2), selecting thee subculture for 30-40 days, and growing strong blueberry; cutting the blueberry to 3-4 cm long stem and placing the base part in the IBA solution, rapidly dipping for 10-15 s; then vertically placing these stems in the culture bottle paved with the filter paper; 3), culturing for a week under the dark light condition, and then culturing for 20-30 days under the condition that the light intensity is 1500-2500 Lx, and light time length is 10-14h / d. The method is fast in rooting speed, high in rooting rate, simple in operation, and suitable for large-scale production.

Description

technical field [0001] The invention relates to the technical field of blueberry tissue culture, in particular to a rapid rooting method for blueberries in a bottle. Background technique [0002] Blueberry belongs to Rhododendronaceae Vaccinium, and it is one of the emerging fruit tree species with high economic value and broad development prospects. Blueberry fruit is rich in nutrients, especially the antioxidant capacity is the first among all fruits and vegetables. [0003] At present, the use of plant tissue culture method can quickly reproduce a variety of plants in a short period of time. Not only the reproduction rate is high, but also because it is asexual reproduction, it can maintain the excellent traits of the original breeding mother plant. In recent years, it has been more and more widely used in production. In addition, cutting, as another important means of seedling breeding, has also been widely valued because of its economy and convenience. However, bluebe...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 肖海峻罗红霞孟利前陈兰芬王伟青郭彤
Owner BEIJING VOCATIONAL COLLEGE OF AGRI
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