Rapid rooting method in blueberry bottle
A blueberry, fast technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of cumbersome operation, long time required for rooting, etc., and achieve the effects of simple operation, high rooting rate, and wide application range.
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Embodiment 1
[0021] Embodiment 1: The test variety is O'Neill
[0022] 1) Spread a layer of filter paper in the culture bottle, then pour the prepared liquid culture medium (the formula is 1 / 2WPM+20g / L sucrose+0.3% activated carbon, pH is 5.8) into the culture bottle, then put the culture bottle and The Erlenmeyer flask containing IBA (concentration of IBA is 200mg / L) is sterilized at 121°C for 15 minutes for later use; 2) select blueberries that have been subcultured for about 35 days and have grown vigorously, and cut them into 3-4cm long blueberries. For the stem segment, put its base into the IBA solution and dip it at a medium speed for 10 seconds, and then put these stem segments vertically into the culture bottle; The cultivation was carried out under the cultivation conditions under d. After 30 days of cultivation under light, the rooting rate is 92%, the average root length is 2.7cm, and the survival rate after transplanting can reach 93%.
Embodiment 2
[0023] Embodiment 2: The test variety is O'Neill
[0024] (1) Spread a layer of filter paper in the culture bottle, then pour the prepared liquid culture medium (the formula is 1 / 2WPM+20g / L sucrose, also contains vitamin C, the addition amount is 0.001mmol / L, pH is 5.8) Put it into the culture bottle, then sterilize the culture bottle and the Erlenmeyer flask (IBA concentration is 200mg / L) with IBA at a high temperature of 121°C for 15min for later use; Cut the stems with a length of 3-4cm, dip their bases in IBA solution for 10 seconds at a medium speed, and then put these stems vertically into culture bottles; Under the culture conditions of 2000Lx and light duration of 14h / d, they were cultivated. After being cultivated under light for 30 days, the rooting rate can reach 90%, the average root length is 2.1cm, and the survival rate after transplanting can reach 92%.
Embodiment 3
[0025] Embodiment 3: The test variety is O'Neill
[0026] (1) Spread a layer of filter paper in the culture bottle, then pour the prepared liquid culture medium (the formula is 1 / 2WPM+20g / L sucrose, pH 5.8) into the culture bottle, and then put the culture bottle and IBA The Erlenmeyer flask (IBA concentration is 200mg / L) is sterilized under the high temperature of 121 ℃ for 15min for later use; 2) select the blueberry that grows vigorously after subculture for 35 days, cut it into the stem segment that is 3-4cm in length, and cut it into Put the base into the IBA solution and dip it at medium speed for 10 seconds, and then put these stems vertically into the culture bottle; 3) Culture in dark light for a week, and then under the culture conditions of light intensity of 2000Lx and light duration of 14h / d down to cultivate. After being cultivated under light for 30 days, the rooting rate is 87%, the average root length is 2.0cm, and the survival rate after transplanting can re...
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