Method for extracting Olea europaea leaf polyphenol by aqueous two phase system

Abstract

The invention discloses a method for extracting Olea europaea leaf polyphenol by an aqueous two phase system. The method comprises the following steps of adding dried and crushed Olea europaea leaves into the aqueous two phase system containing hydrophilic low molecules organic solvent and inorganic salt solution, mixing, performing ultrasonic extraction, and extracting the Olea europaea leaf polyphenol to an upper phase; performing enrichment on the upper phase with macroporous resin, eluting the upper phase with the organic solvent, and concentrating and drying eluant to obtain the Olea europaea leaf polyphenol; the recovery rate of the Olea europaea leaf polyphenol is more than 90 percent, and the purity of the Olea europaea leaf polyphenol is more than 80 percent. The aqueous two phase extraction technique, which is a new isolation technique, is applied to isolation and extraction of the Olea europaea leaf polyphenol by combing with the traditional ultrasonic-assisted organic solvent extraction technique, therefore the method has the advantages that the product yield is high, the product purity is high, extraction is rapid, the condition is mild, equipment is simple, the Olea europaea leaf polyphenol is easy to amplify, and the like.

Description

technical field [0001] The invention relates to the field of plant extracts, in particular to a method for extracting olive leaf polyphenols with a two-phase aqueous system. Background technique [0002] Olive (Olea europea), also known as foreign olive and zidon fruit, is mainly distributed in countries along the Mediterranean Sea. It mainly uses fresh fruit to process it into olive oil known as the "Queen of Vegetable Oil". Analysis of modern biotechnology shows that olive leaves are rich in antioxidant active ingredients, which mainly include olive polyphenols, flavonoids, split iridoids (including oleuropein), and biflavones. And these active substances need to be extracted from the olive leaf, and the extraction method close to the present invention in the prior art is as follows: [0003] The patent number is 201010535347, and the Chinese patent titled "A Method for Efficiently Enriching High-quality Hydroxytyrosol from Olive Leaves" discloses the following preparatio...

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Problems solved by technology

[0006] At present, the preparation method of the above-mentioned olive leaf extract adopts microwave or ultrasonic method to extract the olive leaf extract and then carry out macroporous adsorption resin enrichment, or only use organic solvent extraction and then carry out macroporous adsorption resin enrichment, without high selectivity. , an emerging separation technology that greatly preserves the activity of biological substances-aqueous two-phase extraction technology