A kind of preparation method of olive leaf extract

Abstract

A preparation method of olive leaf extract, comprising the following steps: (1) superfine grinding of olive leaf raw material, continuous countercurrent ultrasonic extraction; (2) extract liquid treatment to obtain centrifuge supernatant and centrifuge residue; (3) centrifuge residue Dissolving, resin adsorption, elution, concentration, and drying to obtain luteolin product; (4) Take step (2) centrifugal supernatant, add ethanol solution, let stand, filter, recover ethanol, add water to dilute, and pass the solution through macroporous resin Adsorption, elution, collection of ethanol eluate, concentration, and drying to obtain hydroxytyrosol products, oleuropein products, and verbascoside products. The invention has high utilization rate of raw materials and is environmentally friendly; the process is simple and the industrialized production is easy to realize. The product prepared by the method has light color, light smell and controllable water solubility, and has a good market prospect in the fields of nutrition, health care and daily chemical industry.

Description

technical field [0001] The invention relates to an extraction and separation process of active components in olive leaf plants, in particular to a preparation method of olive leaf extract. Background technique [0002] Olive, Latin scientific name ( Olea europaea Linn.), a plant of the genus Oleaceae Oleaceae, also known as Olea, Olean, is a famous subtropical fruit tree and an important economic forest tree, mainly distributed in Mediterranean countries, with Greece, Italy, Tunisia, and Spain as concentrated production areas. [0003] The literature "Research Overview of Chemical Composition and Pharmacological Actions of Olive Leaf [J]. Journal of Logistics Academy of Armed Police Force, 2013, 22 (4): 337-340" reported that olive leaves mainly contain flavonoids, terpenoids and their derivatives, phenols Acid compounds, aliphatic compounds four major chemical components. Among them, luteolin belongs to flavonoids, hydroxytyrosol and verbascoside can be classified as phe...

AI Technical Summary

Problems solved by technology

This process adopts the combination of high-pressure micro-jet ultra-fine pulverization and microwave extraction, and the active ingredients are extracted more completely, but there is also the problem of low utilization rate of raw materials

[0009] CN201610883391.5 discloses a process for extracting olive leaf hydroxytyrosol. The method includes the following steps: (1) crush olive leaves into coarse powder, pass through a 60-mesh sieve, and add 80% The ethanol is sealed and swelled for 2 hours, and the fully expanded olive leaf coarse powder is percolated and extracted with 80% ethanol solvent to obtain the extract; (2) The extract in step (1) is absorbed by D101 macroporous resin, After elution with 60% ethanol, the eluate is obtained, and the obtained eluate is concentrated and dried to obtain 45-55% oleuropein; (3) the oleuropein product in step (2) is subjected to hydrogen oxidation The sodium solution is hydrolyzed, and after adding acid to neutralize, the precipitate is centrifuged, and the obtained centrifugate is absorbed by D101 macroporous resin, and then eluted with 60% ethanol at 60-65°C to obtain the eluent; (4) the step (3) After the eluent in (3) is concentrated, it is repeatedly extracted 4 times with the same amount of ethyl acetate, and the ethyl acetate layer is concentrated and dried to obtain 80-90% hydroxytyrosol; the process uses a strong base Solution hydrolyzes oleuropein to prepare hydroxytyrosol, and finally only one product is harvested, and the utilization rate of raw materials is low

At the same time, the process uses acid and alkali to adjust the pH value, which causes great environmental pollution.

[0010] In general, the existing preparation methods of olive leaf extracts are all designed for one active ingredient, and there is insufficient utilization of olive leaf raw materials

Similarly, the preparation method of hydroxytyrosol is mostly obtained by hydrolysis of oleuropein, and the use of organic solvents such as acid and alkali and ethyl acetate and petroleum ether exists in the process, which has great environmental hazards