Method and apparatus used for preparing olive leaf extract

A technology of olive leaf extract and olive leaf, which is applied to the preparation of sugar derivatives, chemical instruments and methods, sugar derivatives, etc., can solve the problems of shortened production cycle, long extraction time, and low efficiency, and achieve shortened production cycle, Good reproducibility and the effect of improving economic benefits

Inactive Publication Date: 2013-01-02
INST OF CHEM IND OF FOREST PROD CHINESE ACAD OF FORESTRY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0011] The invention provides a preparation method of olive leaf extract related to oleuropein and total flavonoids, which solves the problems of long extraction time and low efficiency in the production process of certain purity oleuropein and total flavonoids, and shortens the production cycle

Method used

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  • Method and apparatus used for preparing olive leaf extract
  • Method and apparatus used for preparing olive leaf extract
  • Method and apparatus used for preparing olive leaf extract

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preparation example Construction

[0035] A preparation method of olive leaf extract:

[0036] (1) Extraction: Weigh a certain amount of olive leaf powder (extraction device such as figure 2 shown), then add an appropriate amount of zeolite, use a polar solvent in the olive leaf and solvent ratio of 1:10~1:30 (w / v), extract at 40~50°C, and extract for 1~30 min. Under the conditions of ultrasonic power of 240-1200 W and system vacuum of 30-90 kPa, ultrasonic decompression boiling extraction is carried out, and the polar solvent is a mixed solution of ethanol and deionized water.

[0037] (2) Extraction: vacuum filter the extract in (1) above, transfer the collected supernatant to a separatory funnel, add petroleum ether for extraction, let stand for 10 minutes, and collect the lower supernatant.

[0038] (3) Column separation: Rotate the collected solution in (2) at 30~50°C to recover the solvent to obtain a certain amount of concentrated solution, then dilute it with an appropriate amount of deionized water, ...

Embodiment 1

[0045] (1) Extraction: 100 g of olive leaves that were naturally dried in the shade were crushed with a pulverizer, placed in an ultrasonic decompression extraction device, an appropriate amount of zeolite, 2 L of 75% ethanol were added, the temperature was 50 °C, the ultrasonic power was 600 W, and vacuum Ultrasonic-decompression boiling extraction was carried out under the conditions of 70 kPa and 5 min.

[0046] (2) Extraction: Add the collected extract into a separatory funnel with a capacity of 5 L, then add 500 ml of petroleum ether to extract three times, let stand for 10 min, and collect the lower layer of the layered liquid.

[0047] (3) Column separation: The lower layer liquid collected from the separatory funnel was rotary evaporated at 45°C to recover the alcohol solvent until the concentrated liquid had no alcohol smell, then diluted with 400ml of deionized water, separated by AB-8 macroporous resin, and adsorbed After 30 min, it was eluted with 70% ethanol, and ...

Embodiment 2

[0051] (1) Extraction: Weigh 300 g of naturally shade-dried olive leaves, pulverize them with a pulverizer, put them in an ultrasonic decompression extraction device, add an appropriate amount of zeolite, 3.5 L of 75% ethanol, the temperature is 45°C, and the ultrasonic power is 720 W. Ultrasonic-decompression boiling extraction was carried out under the conditions of vacuum degree of 75 kPa and time of 7 min.

[0052] (2) Extraction: Add the collected extract into a separatory funnel with a capacity of 5 L, then add 850 ml of petroleum ether to extract three times, let stand for 15 min, and collect the lower layer of the layered liquid.

[0053] (3) Column separation: The lower layer liquid collected from the separatory funnel was rotary evaporated at 50°C to recover the ethanol solvent and make the concentrated liquid have no alcohol smell, then added 750 ml of deionized water to dilute, and then separated by AB-8 macroporous resin , after 30 minutes of adsorption, elute wit...

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Abstract

The invention discloses a method and an apparatus used for preparing an olive leaf extract. The method comprises the steps that: olive leaves are subjected to ultrasonic vacuum boiling extraction by using a mixed solvent; vacuum pump-filtration is carried out, such that a clarified liquid is obtained; fat-soluble impurities are removed through petroleum ether extraction, and a lower-layer clear solution is obtained; the clear solution is subjected to rotary evaporation until no alcohol smell is left in the solvent; the solution is diluted; the diluant is subjected to macroporous resin purification; the obtained solution is subjected to standing for a period of time, such that oleuropein and flavonoid substances are sufficiently absorbed; the oleuropein is eluted by using an ethanol-water mixed solvent with a proper concentration, and a target liquid is collected; the collected target liquid is subjected to dextrane gel purification; standing is carried out, and an ethanol-water solution is used for eluting; a eluant containing effective components is collected; the collected eluant is subjected to rotary evaporation until no alcohol smell is left; and the obtained material is dried, such that the extract is obtained. The oleuropein content of the extract reaches 85-92%, and the total flavonoid content of the extract reaches 25-35%. The method has the advantages of simple operation, short extraction time, high efficiency, high product recovery rate, good quality, and stable quality.

Description

technical field [0001] The invention relates to the technical field of extraction and purification of chemical active ingredients of natural products, and relates to a preparation process of olive leaf extract containing high-purity oleuropein and total flavonoids. Background technique [0002] Olive belongs to the Oleaceae family, also known as olean fruit, a subtropical evergreen tree, drought-resistant, cold-resistant, and a long-lived tree species with strong growth ability. Mainly distributed in Mediterranean countries, such as Greece, Italy, Spain and other countries, it is now cultivated almost all over the world. It was introduced into China in the 1960s and is now widely distributed in Yunnan, Gansu, Guangdong, Guangxi, Sichuan, Shaanxi and other places. [0003] Olive oil is famous for its ability to extract olive oil from its fruit. Olive oil contains many biologically active substances, such as unsaturated fatty acids, vitamin E, oleuropein, and hydroxytyrosol....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/04C07H1/08C07G99/00
Inventor 黄立新谢普军张彩虹游凤王成章周昊
Owner INST OF CHEM IND OF FOREST PROD CHINESE ACAD OF FORESTRY
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