Separation and preparation method of high-purity oleuropein

An oleuropein and high-purity technology, which is applied in the preparation of sugar derivatives, chemical instruments and methods, sugar derivatives, etc., can solve the problems of poor purity of oleuropein and uneven product quality, and achieve the effect of high purity
CN113754712APending Publication Date: 2021-12-07LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Publication Date
2021-12-07

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Abstract

The invention discloses a separation and preparation method of high-purity oleuropein. The separation and preparation method comprises the following steps of performing heating reflux extraction on olive leaves with water to obtain an oleuropein crude extract; carrying out separation and enrichment on the oleuropein crude extract by using macroporous adsorption resin to obtain an oleuropein eluate; separating and purifying the oleuropein eluate by using a high-speed counter-current chromatography separation technology to obtain an oleuropein crude product; separating and purifying the oleuropein crude product by using preparative liquid chromatography to obtain an oleuropein refined product; and freeze-drying the oleuropein refined product to obtain the high-purity oleuropein. The purity of the oleuropein separated and extracted by the method is high and is greater than 98.5%, and the high-purity oleuropein can be used as a standard sample of the oleuropein.
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Description

technical field

[0001] The invention relates to a method for separating and preparing high-purity oleuropein, belonging to the technical field of extracting medicinal components. Background technique

[0002] Olive (Olea europaea L.) is an evergreen tree of Oleaceae Olea, mainly distributed in countries along the Mediterranean Sea. Olive leaves have a long history of cultivation and consumption in European and American countries. Dry leaf tea, powder or extracts are often taken orally for lowering blood pressure, lowering blood sugar, anti-inflammatory and anti-oxidation. The main medicinal component of olive is oleuropein, which has the highest content in olive leaves, up to 4%-12%.

[0003] Oleuropein, CAS: 32619-42-4, molecular formula: C 25 h 32 o 13 , Molecular weight: 540.51, Chemical name: 2H-Pyran-4-acetic acid, 3-ethylene-2-(β-D-glucopyranosyloxy) -3,4-dihydro-5-(methoxycarbonyl)-,2-(3,4 -dihydroxyphenyl)ethyl ester, (2S,3E,4S), properties: white powder, meltin...

Claims

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