Separation and preparation method of high-purity oleuropein

An oleuropein and high-purity technology, which is applied in the preparation of sugar derivatives, chemical instruments and methods, sugar derivatives, etc., can solve the problems of poor purity of oleuropein and uneven product quality, and achieve the effect of high purity

Pending Publication Date: 2021-12-07
LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, at present, there is a lack of standardized and unified standard samples of oleuropein in the domestic market, the quality of related products varies, and the purity of oleuropein is relatively poor

Method used

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  • Separation and preparation method of high-purity oleuropein
  • Separation and preparation method of high-purity oleuropein
  • Separation and preparation method of high-purity oleuropein

Examples

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Embodiment 1

[0046] Example 1: A method for the separation and preparation of high-purity oleuropein

[0047] Include the following steps:

[0048] (1) Use the dried leaves of olive (Olea europaea L.) as raw materials, crush them and pass through a 10-mesh sieve to obtain olive leaf meal powder, add 5 times the weight of water to the olive leaf meal powder, heat to about 55°C and reflux for extraction for 6 hours A crude oleuropein extract (yield rate 37%, extraction rate 85%) was obtained, and the content of oleuropein in the crude oleuropein extract was 12%.

[0049] (2) The crude oleuropein extract obtained in step (1) is adsorbed on D101 macroporous adsorption resin, and the mass ratio of the crude oleuropein extract to the macroporous adsorption resin is 1:50, and the column flow rate is 1ml / min, the flow rate of the eluent on the column is 1ml / min, and the amount of eluent is 2BV; after the adsorption is completed, it is eluted with water, 50% ethanol and 95% ethanol in sequence, an...

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Abstract

The invention discloses a separation and preparation method of high-purity oleuropein. The separation and preparation method comprises the following steps of performing heating reflux extraction on olive leaves with water to obtain an oleuropein crude extract; carrying out separation and enrichment on the oleuropein crude extract by using macroporous adsorption resin to obtain an oleuropein eluate; separating and purifying the oleuropein eluate by using a high-speed counter-current chromatography separation technology to obtain an oleuropein crude product; separating and purifying the oleuropein crude product by using preparative liquid chromatography to obtain an oleuropein refined product; and freeze-drying the oleuropein refined product to obtain the high-purity oleuropein. The purity of the oleuropein separated and extracted by the method is high and is greater than 98.5%, and the high-purity oleuropein can be used as a standard sample of the oleuropein.

Description

technical field [0001] The invention relates to a method for separating and preparing high-purity oleuropein, belonging to the technical field of extracting medicinal components. Background technique [0002] Olive (Olea europaea L.) is an evergreen tree of Oleaceae Olea, mainly distributed in countries along the Mediterranean Sea. Olive leaves have a long history of cultivation and consumption in European and American countries. Dry leaf tea, powder or extracts are often taken orally for lowering blood pressure, lowering blood sugar, anti-inflammatory and anti-oxidation. The main medicinal component of olive is oleuropein, which has the highest content in olive leaves, up to 4%-12%. [0003] Oleuropein, CAS: 32619-42-4, molecular formula: C 25 h 32 o 13 , Molecular weight: 540.51, Chemical name: 2H-Pyran-4-acetic acid, 3-ethylene-2-(β-D-glucopyranosyloxy) -3,4-dihydro-5-(methoxycarbonyl)-,2-(3,4 -dihydroxyphenyl)ethyl ester, (2S,3E,4S), properties: white powder, meltin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/04C07H1/08
CPCC07H17/04C07H1/08
Inventor 裴栋邸多隆
Owner LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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