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Oleuropein-enriched olea europaea leaf polyphenol extract feed additive with effect of improving broiler chicken quality and preparation method thereof

A feed additive, olive leaf technology, applied in the field of olive leaf polyphenol extract feed additive and its preparation, to achieve the effect of improving meat quality, reducing MDA content, and easy operation

Pending Publication Date: 2021-12-31
INST OF CHEM IND OF FOREST PROD CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Patents filed domestically in recent years have found that a toothpaste prepared from olive extracts and its preparation method (CN201510225644.5), a feed additive to improve the reproductive performance of male ducks (CN201710365462.7), and the use of olive leaf extract As feed additives, there are few reports

Method used

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  • Oleuropein-enriched olea europaea leaf polyphenol extract feed additive with effect of improving broiler chicken quality and preparation method thereof
  • Oleuropein-enriched olea europaea leaf polyphenol extract feed additive with effect of improving broiler chicken quality and preparation method thereof
  • Oleuropein-enriched olea europaea leaf polyphenol extract feed additive with effect of improving broiler chicken quality and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] This embodiment takes an olive leaf polyphenol extract feed additive rich in oleuropein that can improve the quality of broiler chickens and its preparation method as an example.

[0037] (1) Preparation of olive leaf polyphenol extract

[0038] At the end of November, the Laixing olive leaves picked at an altitude of 900 meters in Longnan area were dried and crushed naturally out of sunlight, and 10 kg of leaf powder was weighed, and 100 L of ethanol-water with a volume fraction of 75% was added to the extraction kettle. Mechanical stirring under high temperature (speed 1500 r / min), supplemented by microwave enhanced extraction, set the power 1000 W to extract for 10 min, suction filtration while hot, the collected filtrate was concentrated by rotary evaporation, passed through AB-8 macroporous resin (elution The concentration range of the solvent is 70% ethanol-water, and the flow rate is 5.0 mL / min.), and then concentrated by rotary evaporation, adding an appropriate...

Embodiment 2

[0043] This embodiment takes an olive leaf polyphenol extract feed additive rich in oleuropein that can improve the quality of broiler chickens and its preparation method as an example.

[0044] (1) Preparation of olive leaf polyphenol extract

[0045] At the end of November, the Laixing olive leaves were picked from the Longnan area at an altitude of 1100 meters, and were naturally air-dried and crushed out of the sun. The leaf powder was weighed 9 kg, and 87 L of ethanol-water with a volume fraction of 85% was added to the extraction kettle. Mechanical stirring (at a rate of 2000 r / min) was carried out at the same time, supplemented by microwave-enhanced extraction, set the power of 1200 W to extract for 15 min, suction filtered while it was hot, collected the filtrate and concentrated it by rotary evaporation, and diluted the concentrated solution with water to 12% olive bitter Glycosides, passed through AB-8 macroporous resin (the concentration range of the elution solvent...

Embodiment 3

[0050] This embodiment takes an olive leaf polyphenol extract feed additive rich in oleuropein that can improve the quality of broiler chickens and its preparation method as an example.

[0051] (1) Preparation of olive leaf polyphenol extract

[0052] At the end of November, olive leaves from Moyola at an altitude of 1,250 meters in the southern Longnan area were picked and crushed by natural air-drying out of the sun. Weighed 8 kg of leaf powder and mechanically stirred at room temperature (at a rate of 3,000 r / min). Intensify the extraction with microwave, set the power to 1500 W to extract for 20 min, suction filter while it is hot, collect the filtrate and concentrate by rotary evaporation, dilute the concentrated solution with water to 12% oleuropein, pass through D101 macroporous resin (the concentration range of the elution solvent is 90 % ethanol-water, the flow rate is 10.0 mL / min.), and then concentrated by rotary evaporation, adding an appropriate amount of water f...

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Abstract

The invention discloses anoleuropein-enriched olea europaea leaf polyphenol extract feed additive with effect of improving broiler chicken quality and a preparation method thereof. The olea europaea leaf polyphenol extract feed additive is prepared by the following steps that natural air-dried olea europaea leaves collected from the southern Gansu region at the altitude of 900-1250m are taken as the raw material, an alcohol-water system is used as an extraction solvent, rapid extraction is performed under the assistance of mechanical stirring and microwave, filtration, column chromatography separation, rotary evaporation concentration and spray drying are performed while the materials are hot to obtain the anoleuropein-enriched olea europaea leaf polyphenol extract. When the prepared olea europaea leaf polyphenol extract is applied to a broiler feed additive, the oxidation resistance can be remarkably improved, the intestinal micro-ecological environment of the broiler chicken is obviously improved, and the quality of the broiler chicken is finally improved.

Description

technical field [0001] The invention relates to the field of directional enrichment of active ingredients in natural products and animal feed additives, in particular to an olive leaf polyphenol extract feed additive rich in oleuropein that can improve broiler quality and a preparation method thereof. Background technique [0002] The radiation of drug residues, enhanced drug resistance, and potential safety hazards to the human body due to the extensive use of antibiotics are prompting the scientific community to look for natural, safe, and efficient feed additives to replace traditional antibiotics. Abundant plant resources provide sufficient conditions for the screening of natural feed additives. Among them, the characteristic forest resource represented by olive oil is an important source of natural feed additives. This is because olives are rich in effective antibacterial active substances, which have been used as active ingredients for anti-oxidation, antibacterial, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/75A23K10/30B01D11/02B01J19/12
CPCA23K50/75A23K10/30B01D11/0288B01D11/0257B01D11/02B01J19/126
Inventor 谢普军黄立新张彩虹
Owner INST OF CHEM IND OF FOREST PROD CHINESE ACAD OF FORESTRY
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