Microwave-assisted aqueous two-phase extraction and separation method of kudzu root total flavones

A microwave-assisted technology of total flavonoids of Pueraria radiata, which is applied in the direction of medical preparations containing active ingredients, pharmaceutical formulas, plant/algae/fungus/moss components, etc., can solve the problems of unsuitable large-scale industrial production, high cost of polyethylene glycol, The problem of high viscosity of the system can be solved, and the effect of easy process scale-up and continuous operation, no obvious scale-up effect and small interfacial tension can be achieved.

Inactive Publication Date: 2013-12-11
WUHAN INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although the two-phase aqueous system formed by polymers has high extraction efficiency, the cost of polyethylene glycol (referred to as PEG) and dextran (referred to as Dextran) is too high, and the system has high viscosity and is difficult to recycle, so the cost is high. Not suitable for industrial mass production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Preparation of two-phase aqueous system

[0035] Take 12gK 2 HPO 4 ·3H 2 O, add 22mL of water to dissolve, add 18mL of ethanol to prepare a two-phase extractant;

[0036] (2) Microwave-assisted aqueous two-phase extraction

[0037] Add 0.2 g of pueraria root powder that was precisely weighed through a 60-mesh sieve to the two-phase aqueous system obtained in step (1), set the microwave conditions at 60°C, 10 min, and 500W, and perform microwave-assisted two-phase extraction. After the extraction, The Pueraria flavonoids extract that was obviously phase-separated by suction filtration.

[0038] (3) Use ultraviolet spectrophotometry to measure the concentration of total flavonoids in Pueraria root in the two-phase extraction system, and calculate the recovery rate according to the formula (3):

[0039] R=V 上 / V 下 (1)

[0040] K=C 上 / C 下 (2)

[0041] Y=C 上 ×V 上 / M×100% (3)

[0042] In the formula: R is compared; V 上 and V 下 is the volume of the ...

Embodiment 2

[0046] (1) Preparation of two-phase aqueous system

[0047] Take 16gK 2 HPO 4 ·3H 2 O, add 22mL of water to dissolve, add 18mL of ethanol to prepare a two-phase extractant;

[0048] (2) Microwave-assisted aqueous two-phase extraction

[0049] Add 0.2g of pueraria root powder that has passed through a 60-mesh sieve to the two-phase aqueous system obtained in step (1), and set the microwave conditions at 60°C, 10min, and 600W to carry out microwave-assisted two-phase extraction. After the extraction, The Pueraria flavonoids extract that was obviously phase-separated by suction filtration. The yield of Pueraria flavonoids was 12.92%.

Embodiment 3

[0051] (1) Preparation of two-phase aqueous system

[0052] Take 16gK 2 HPO 4 ·3H 2 O, add 22mL of water to dissolve, add 18mL of ethanol to prepare a two-phase extractant;

[0053] (2) Microwave-assisted aqueous two-phase extraction

[0054] The two-phase aqueous system obtained in step (1) was added with precisely weighed 1 g of kudzu root powder passed through a 60-mesh sieve, and the microwave conditions were set at 60°C, 10min, and 600W to carry out microwave-assisted two-phase extraction. The Pueraria flavonoids extract that was clearly phase-separated was filtered. The yield of Pueraria flavonoids was 12.72%.

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Abstract

The invention relates to a microwave-assisted aqueous two-phase extraction method for separating kudzu root total flavone, which comprises the following steps: 1) preparation of an aqueous two-phase system: taking an inorganic salt, adding water to dissolve the inorganic salt, and adding an organic solvent to obtain a aqueous two-phase extractant; and 2) microwave-assisted aqueous two-phase extraction: adding kudzu root powder into the aqueous two-phase extractant obtained in the step 1), carrying out microwave-assisted extraction, and carrying out vacuum filtration to obtain an obvious phase-separated kudzu root flavone extracting solution. Compared with the prior art, the method provided by the invention has the following advantages: (1) the method is simple to operate and does not need to wait for phase separation; 2) the process is integrated; 3) the extraction process is quick and efficient; 4) the extraction solvent is low in toxicity; 5) the interfacial tension is small, thereby being beneficial to mass transfer between the two phases; 6) abundant impurities can be removed along with solid matters, thereby being beneficial to purifying the sample; and 7) the extraction method does not have obvious scale-up effect, can easily implement technique amplification and continuous operation, and can be directly connected with the subsequent purification procedure without special treatment.

Description

technical field [0001] The invention belongs to the field of extraction, separation and purification of effective components of natural products, and in particular relates to a method for microwave-assisted two-water phase extraction and separation of total flavonoids of kudzu root. Background technique [0002] Pueraria lobata is the dried root of the leguminous plant Pueraria mirifica. It is a commonly used Chinese medicinal material. It has been recorded in herbal medicines throughout the ages. It is sweet, pungent, and flat in nature. Clear the meridian and activate the collaterals, relieve alcohol poisoning. At present, kudzu root is widely used to improve cardiovascular and cerebrovascular diseases. It is used in a large amount in important compound preparations such as diarrhea and diabetes, and is an important component of the preparation. There are more than 140 kinds of kudzu root compound preparations included in the Pharmacopoeia of the People's Republic of Chin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/488A61K8/97A23L1/30A23L33/105
Inventor 池汝安张越非刘哲李娅丽余军霞
Owner WUHAN INSTITUTE OF TECHNOLOGY
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