Method for extracting phenylethanoid glycoside in herba cistanche by using salt-induced deep-eutectic solvent

A technology of deep eutectic solvent and phenylethanol glycosides, which is applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve the problem that the two-phase aqueous system cannot be reused, the organic solvent is volatile, and the non-process time is prolonged and other problems, to achieve the effect of mild extraction and extraction environmental conditions, environmental friendliness, and good solubility

Active Publication Date: 2017-05-31
HEXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In the prior art, solvent extraction is generally used to extract phenylethanol glycosides from Cistanche deserticola. For example, CN105017344 and CN102441040 disclose a process for extracting phenylethanol glycosides from Cistanche deserticola. Organic solvents are used to reflux the extracts, and macropores are used to extract them. Adsorption resin separates phenylethanol glycosides, but the macroporous resin needs more pretreatment steps and is time-consuming; another example is CN101041677, which uses membrane separation technology to produce raw materials containing phenylethanol glycosides from Cistanche deserticola and its preparation method, but the cost of membrane equipment is higher , and it is easy to cause membrane pollution and blockage, the membrane needs to be physically or chemica

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  • Method for extracting phenylethanoid glycoside in herba cistanche by using salt-induced deep-eutectic solvent
  • Method for extracting phenylethanoid glycoside in herba cistanche by using salt-induced deep-eutectic solvent
  • Method for extracting phenylethanoid glycoside in herba cistanche by using salt-induced deep-eutectic solvent

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Embodiment 1

[0035] A method for salt-induced deep eutectic solvent extraction of phenylethanol glycosides in Cistanche deserticola, the method comprising the steps of:

[0036] S1: Add a deep eutectic solvent aqueous solution with a mass fraction of 65% to 1 g of Cistanche deserticola powder. Composed of choline and sorbitol, microwave extraction, the microwave extraction power is 150W, the extraction time is 4min, and filtered to obtain the crude extract of Cistanche deserticola;

[0037] S2: Aqueous two-phase extraction: adding K to the Cistanche cistanche crude extract obtained in step S1 3 PO 4 , mix well at room temperature to obtain a two-phase aqueous system, adjust the pH to 8, let stand, separate the phases at room temperature for 12 minutes, separate the upper phase and the lower phase; in the two-phase aqueous system, K 3 PO 4 The mass fraction of is 60%, and the mass fraction of the deep eutectic solvent in the two-phase system is 28%;

[0038] S3: Secondary aqueous two-ph...

Embodiment 2

[0041] A method for salt-induced deep eutectic solvent extraction of phenylethanol glycosides in Cistanche deserticola, the method comprising the steps of:

[0042] S1: Add a deep eutectic solvent aqueous solution with a mass fraction of 60% to 1 g of Cistanche deserticola powder, the solid-liquid ratio of Cistanche deserticola powder and the deep eutectic solvent aqueous solution is 1:15, and the molar ratio of the deep eutectic solvent is 1:2:1 Composed of choline chloride, glycerin and oxalic acid, microwave extraction, the microwave extraction power is 200W, the extraction time is 3min, and filtered to obtain the crude extract of Cistanche deserticola;

[0043] S2: Aqueous two-phase extraction: adding K to the Cistanche cistanche crude extract obtained in step S1 2 HPO 4 , mix well at room temperature to obtain a two-phase aqueous system, adjust the pH to 6, let stand, separate the phases at room temperature for 10 minutes, separate the upper phase and the lower phase; in...

Embodiment 3

[0047] A method for salt-induced deep eutectic solvent extraction of phenylethanol glycosides in Cistanche deserticola, the method comprising the steps of:

[0048] S1: Add 50% deep eutectic solvent aqueous solution to 1 g of Cistanche deserticola powder, the solid-liquid ratio of Cistanche deserticola powder and deep eutectic solvent aqueous solution is 1:20, and the molar ratio of deep eutectic solvent is 1:1:1 Composed of choline chloride, glucose and glycerin, microwave extraction, microwave extraction power is 100W, extraction time is 5min, filtered to obtain the crude extract of Cistanche deserticola;

[0049] S2: Aqueous two-phase extraction: adding K to the Cistanche cistanche crude extract obtained in step S1 3 PO 4 , mix well at room temperature to obtain a two-phase aqueous system, adjust the pH to 7.5, let stand, separate the phases at room temperature for 15 minutes, separate the upper phase and the lower phase; in the two-phase aqueous system, K 3 PO 4 The mas...

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Abstract

The invention provides a method for extracting phenylethanoid glycoside in herba cistanche by using a salt-induced deep-eutectic solvent on the one hand. According to the method, the phenylethanoid glycoside in the herba cistanche is extracted by using a water solution of the deep-eutectic solvent to extract herba cistanche powder, and adding phosphate into extracting liquid to prepare an aqueous two-phase system. According to the method, conditions of the extraction environment are mild, and phase separation time is short; the yield of the phenylethanoid glycoside in the herba cistanche can reach up to 20%.

Description

technical field [0001] The invention belongs to the field of separation and purification of active ingredients of natural products, and in particular relates to a method for extracting phenylethanol glycosides from Cistanche deserticola with a salt-induced deep eutectic solvent. Background technique [0002] Cistanche deserticola (Cistanche deserticola Y.C.Ma), also known as Cistanche deserticola, Xinjiang Yun, Cun Yun, etc., is a perennial parasitic plant of Orobanchaceae (Cistanche Hoffmg.et Link). Activity, the relevant pharmacological and physiological activities that have been found include: hormone regulation, laxative, liver protection, neuroprotection, myocardial ischemia protection, immune regulation, and bone formation promotion. The main chemical components of Cistanche deserticola are: phenylethanol glycosides, benzyl alcohol Glycosides, iridoids, monoterpenes, lignans and phenylpropanols, alkaloids, amino acids, sugars, sterols and volatile oil components. Among...

Claims

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Application Information

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IPC IPC(8): C07H15/18C07H1/08
CPCC07H1/08C07H15/18
Inventor 张喜峰杨生辉王丹霞罗光宏陈天仁
Owner HEXI UNIV
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