A method for salt-induced deep eutectic solvent extraction of phenylethanol glycosides in Cistanche cistanche

Abstract

The invention provides a method for extracting phenylethanoid glycoside in herba cistanche by using a salt-induced deep-eutectic solvent on the one hand. According to the method, the phenylethanoid glycoside in the herba cistanche is extracted by using a water solution of the deep-eutectic solvent to extract herba cistanche powder, and adding phosphate into extracting liquid to prepare an aqueous two-phase system. According to the method, conditions of the extraction environment are mild, and phase separation time is short; the yield of the phenylethanoid glycoside in the herba cistanche can reach up to 20%.

Description

technical field [0001] The invention belongs to the field of separation and purification of active ingredients of natural products, and in particular relates to a method for extracting phenylethanol glycosides from Cistanche deserticola with a salt-induced deep eutectic solvent. Background technique [0002] Cistanche deserticola (Cistanche deserticola Y.C.Ma), also known as Cistanche deserticola, Xinjiang Yun, Cun Yun, etc., is a perennial parasitic plant of Orobanchaceae (Cistanche Hoffmg.et Link). Activity, the relevant pharmacological and physiological activities that have been found include: hormone regulation, laxative, liver protection, neuroprotection, myocardial ischemia protection, immune regulation, and bone formation promotion. The main chemical components of Cistanche deserticola are: phenylethanol glycosides, benzyl alcohol Glycosides, iridoids, monoterpenes, lignans and phenylpropanols, alkaloids, amino acids, sugars, sterols and volatile oil components. Among...

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Problems solved by technology

[0003] In the prior art, solvent extraction is generally used to extract phenylethanol glycosides from Cistanche deserticola. For example, CN105017344 and CN102441040 disclose a process for extracting phenylethanol glycosides from Cistanche deserticola. Organic solvents are used to reflux the extracts, and macropores are used to extract them. Adsorption resin separates phenylethanol glycosides, but the macroporous resin needs more pretreatment steps and is time-consuming; another example is CN101041677, which uses membrane separation technology to produce raw materials containing phenylethanol glycosides from Cistanche deserticola and its preparation method, but the cost of membrane equipment is higher , and it is easy to cause membrane pollution and blockage, the membrane needs to be physically or chemically cleaned to prolong the non-process time; and CN104189100 discloses a method for extracting phenylethanol glycosides from Cistanche deserticola in two aqueous phases, which is to add Cistanche deserticola powder to ethanol / ammonium sulfate, ethanol / dipotassium hydrogen phosphate, ethanol / sodium citrate or PEG / ammonium sulfate system and other two-phase aqueous systems for extraction. This method has the advantages of mild conditions, high water content, high efficiency, and low energy consumption. However, this method still has problems such as the volatilization of organic solvents, the inability to reuse the aqueous two-phase system, unstable properties, and pollution to the environment; And in the obtained phenylethanol glycosides, the total content of echinacoside and verbascoside is relatively low

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