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Method for separating and purifying beet red pigment by two aqueous phase extraction system

A beet red pigment, separation and purification technology, applied in the field of biochemical industry, can solve the problems of difficult separation of sugar impurities, and achieve the effects of large processing capacity, high speed and reduced energy consumption

Active Publication Date: 2010-06-16
QINGDAO PENGYUAN KANGHUA NATURAL PROD
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Problems solved by technology

[0005] The purpose of the present invention is to provide a method for separating and purifying beet red pigment by two-phase extraction, which solves the problem that beet red pigment is difficult to separate from sugar impurities with similar molecular weight in the crude extract of beet red

Method used

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Embodiment Construction

[0012] The following is an example of this patent.

[0013] (1) Preparation of beet red crude extract: cut the beet root into 5mm thick slices, use 0.05mol / L hydrochloric acid aqueous solution as the extraction agent, solid-liquid ratio 1:3, extract at room temperature for 12h, and extract three times in this way , combined extracts, filtered with filter paper.

[0014] (2) two-phase extraction: adjust the pH value of beet red crude extract to the range of 3, add polyethylene glycol 6000 and ammonium sulfate respectively, so that the mass concentration of polyethylene glycol in the formed two-phase extraction system The concentration of ammonium sulfate is 25%, the mass concentration of ammonium sulfate is 15%, stirring and extracting for 30min, and then standing for 12h, the upper layer is polyethylene glycol aqueous phase containing pigment, and the lower layer is ammonium sulfate aqueous phase containing carbohydrate impurities.

[0015] (3) Separation of beet red pigment ...

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Abstract

The invention relates to a method for separating and purifying a beet red pigment, in particular to a method for purifying a crude extract of the beet red pigment by a two aqueous phase extraction method. The method is characterized by comprising the following steps of: soaking the beet tuberous roots with acidic aqueous solution for extracting the beet red pigment and obtaining a pigment crude extract; then adjusting the pH; adding polyethylene glycol and ammonium sulfate to form a two aqueous phase system; fully mixing and extracting the mixture; forming two phases after standing, wherein the beet red pigment enters a top phase of the polyethylene glycol, and saccharide impurities enter a bottom phase of the ammonium sulfate; separating the beet red pigment from the polyethylene glycol in the top phase by using an ultrafiltration membrane method; concentrating the beet red pigment by using inverse penetration or nanofiltration membranes; then concentrating the beet red pigment in vacuum until the solid content of the beet red pigment is over 20 percent; and finally obtaining a beet red pigment product by using a spray drying method.

Description

technical field [0001] The invention relates to a method for separating and purifying beet red pigment, in particular to a method for separating and purifying beet red pigment by using a two-phase extraction system, and belongs to the technical field of biochemical industry. Background technique [0002] Beet pigments are mainly water-soluble pigments extracted from the tubers of Chenopodiaceae red beet (Beta vulagaris L.), including Betacyanines and a small amount of Betaxanthines. The bright color of beet red pigment is not only a good coloring agent, but also has the functions of anti-oxidation and prevention of cardiovascular diseases. It has broad application prospects in food, medicine, cosmetics and other fields. [0003] During the production of beet red pigment, the crude extract contains a large amount of sugar impurities, which greatly affects the quality and stability of the pigment. At present, there are existing patents for the preparation of beet red pigment,...

Claims

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Application Information

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IPC IPC(8): C09B61/00C09B67/10
Inventor 刘晶晶任莉红郭仁红展鹏远
Owner QINGDAO PENGYUAN KANGHUA NATURAL PROD
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