Method for concentrating and separating flavonoids from toona sinensis leaves
A technology of Chinese toon leaf flavonoids and Chinese toon leaves, which is applied in the field of enrichment and separation of Chinese toon leaf flavonoids in a two-phase system, can solve the problems of high price of macromolecular polymers, high cost of flavonoids, low toxicity of acetone, etc. Achieve the effects of shortening the experiment cycle, easy to master, and not affected by the environment
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Embodiment 1
[0022] (1) Extraction of Toon Leaf Flavonoids
[0023] Toona sinensis leaves were dried and crushed, and added to Φ(C 2 h 5 OH) = 70% ethanol solution, reflux extraction 4 times, 1.5h each time, filter and remove impurities to obtain the crude extract of Toona sinensis leaves, vacuum concentrate to about 1 / 6 of the original volume to obtain a concentrated solution.
[0024] (2) Two-phase enrichment and separation of flavonoids from Toona sinensis leaves
[0025] Take Chinese toon leaf concentrate 9mL, add 3.1g of ammonium sulfate and 1mL of ethanol, mix well, bathe in water at 45°C for 2h, shake continuously during the period, separate the upper and lower phases while hot after phase separation, cool to room temperature and measure the volume of the upper and lower phases, which are 3.36mL respectively Compared with 8.00mL (upper and lower phase volume ratio, the same below) 0.420. Measure respectively (spectrophotometry, hereinafter the same) the flavone concentration of t...
Embodiment 2
[0029] (1) Extraction of Toon Leaf Flavonoids
[0030] Chinese toon leaves are dried or shade-dried, crushed, and added to Φ(C 2 h 5 OH)=70% ethanol solution, reflux extraction 4 times, 1.5h each time, filter and remove impurities to obtain the crude extract of Chinese toon leaves, vacuum concentrate to about 1 / 6 of the original volume to obtain a concentrated solution, and measure the concentration of flavonoids in the concentrated solution to be 3238.011 mg / L.
[0031] (2) Two-phase enrichment and separation of flavonoids from Toona sinensis leaves
[0032] Take Chinese toon leaf concentrate 16mL, add 5.2g of ammonium sulfate and 4mL of ethanol to mix, place in a water bath at 40°C for 3 hours, shake continuously during the period, separate the upper and lower phases while hot after phase separation, cool to room temperature and measure the volume of the upper and lower phases, respectively 10.00mL and 12.20 mL, compared to 0.820. The concentration of flavonoids in the u...
Embodiment 3
[0036] (1) Extraction of Toon Leaf Flavonoids
[0037] Toona sinensis leaves were dried in the shade, crushed, weighed 20g, added 70% ethanol solution at a material-to-liquid ratio of 1:12, and extracted 4 times by microwave at 80°C for 20min each time. After filtering to remove impurities, the crude extract of Toona sinensis leaves was obtained, and concentrated in vacuum to about 1 / 6 of the original volume to obtain a concentrated solution.
[0038] (2) Two-phase enrichment and separation of flavonoids from Toona sinensis leaves
[0039]Take Chinese toon leaf concentrate 7mL, add 2.0g of ammonium sulfate and 3mL of ethanol, bathe in water at 45°C for 2 hours, shake continuously during the period, separate the upper and lower phases while hot after phase separation, cool to room temperature and measure the volume of the upper and lower phases, which are 6.30mL and 4.50 mL, compared to 1.400. The concentrations of flavonoids in the upper and lower phases were measured to be ...
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