Method for concentrating and separating flavonoids from toona sinensis leaves

A technology of Chinese toon leaf flavonoids and Chinese toon leaves, which is applied in the field of enrichment and separation of Chinese toon leaf flavonoids in a two-phase system, can solve the problems of high price of macromolecular polymers, high cost of flavonoids, low toxicity of acetone, etc. Achieve the effects of shortening the experiment cycle, easy to master, and not affected by the environment

Inactive Publication Date: 2009-04-01
GUANGXI UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0005] When flavonoids are enriched and separated by the two-phase aqueous phase formed by polymers and salts, flavonoids are enriched in the macromolecular polymer phase. In order to recover macromolecular polymers, flavonoids must be separated by extraction or ultrafiltration The macromolecular polymers in flavonoids are not only technically cumbersome, but also the cost of extracting flavonoids is higher due to the higher price of macromolecular polymers.
with acetone-K 2 HPO 4 When enriching and separating flavonoids in a two-phase system, flavonoids are enriched in the acetone phase. Although acetone is a small molecule, it is easier to recover than macromolecular polymers, but acetone is low in toxicity and expensive, and remains in the product affect product safety

Method used

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  • Method for concentrating and separating flavonoids from toona sinensis leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Extraction of Toon Leaf Flavonoids

[0023] Toona sinensis leaves were dried and crushed, and added to Φ(C 2 h 5 OH) = 70% ethanol solution, reflux extraction 4 times, 1.5h each time, filter and remove impurities to obtain the crude extract of Toona sinensis leaves, vacuum concentrate to about 1 / 6 of the original volume to obtain a concentrated solution.

[0024] (2) Two-phase enrichment and separation of flavonoids from Toona sinensis leaves

[0025] Take Chinese toon leaf concentrate 9mL, add 3.1g of ammonium sulfate and 1mL of ethanol, mix well, bathe in water at 45°C for 2h, shake continuously during the period, separate the upper and lower phases while hot after phase separation, cool to room temperature and measure the volume of the upper and lower phases, which are 3.36mL respectively Compared with 8.00mL (upper and lower phase volume ratio, the same below) 0.420. Measure respectively (spectrophotometry, hereinafter the same) the flavone concentration of t...

Embodiment 2

[0029] (1) Extraction of Toon Leaf Flavonoids

[0030] Chinese toon leaves are dried or shade-dried, crushed, and added to Φ(C 2 h 5 OH)=70% ethanol solution, reflux extraction 4 times, 1.5h each time, filter and remove impurities to obtain the crude extract of Chinese toon leaves, vacuum concentrate to about 1 / 6 of the original volume to obtain a concentrated solution, and measure the concentration of flavonoids in the concentrated solution to be 3238.011 mg / L.

[0031] (2) Two-phase enrichment and separation of flavonoids from Toona sinensis leaves

[0032] Take Chinese toon leaf concentrate 16mL, add 5.2g of ammonium sulfate and 4mL of ethanol to mix, place in a water bath at 40°C for 3 hours, shake continuously during the period, separate the upper and lower phases while hot after phase separation, cool to room temperature and measure the volume of the upper and lower phases, respectively 10.00mL and 12.20 mL, compared to 0.820. The concentration of flavonoids in the u...

Embodiment 3

[0036] (1) Extraction of Toon Leaf Flavonoids

[0037] Toona sinensis leaves were dried in the shade, crushed, weighed 20g, added 70% ethanol solution at a material-to-liquid ratio of 1:12, and extracted 4 times by microwave at 80°C for 20min each time. After filtering to remove impurities, the crude extract of Toona sinensis leaves was obtained, and concentrated in vacuum to about 1 / 6 of the original volume to obtain a concentrated solution.

[0038] (2) Two-phase enrichment and separation of flavonoids from Toona sinensis leaves

[0039]Take Chinese toon leaf concentrate 7mL, add 2.0g of ammonium sulfate and 3mL of ethanol, bathe in water at 45°C for 2 hours, shake continuously during the period, separate the upper and lower phases while hot after phase separation, cool to room temperature and measure the volume of the upper and lower phases, which are 6.30mL and 4.50 mL, compared to 1.400. The concentrations of flavonoids in the upper and lower phases were measured to be ...

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Abstract

The invention relates to a method for enriching and separating flavones in cedrela sinensis leaves by an aqueous two-phase system, the cedrela sinensis leaves are firstly dried and crushed, crude extract liquid of the cedrela sinensis leaves is obtained after reflux extraction and impurity removal, ammonium sulfate and ethanol are added according to the proportion after concentration, heating and the even mixing are carried out, thereby being split into upper and lower phases; the flavones are mainly distributed in the upper phase containing the ethanol and further concentrated and purified. The lower phase is cooled, crystallized and filtered after splitting the upper and the lower phases, ammonium sulfate crystals are recovered; anhydrous ethanol is added in the upper phase liquid for precipitating, removing the ammonium sulfate and other residual impurities and then recovering the ethanol, and a product is obtained by vacuum drying the concentrated liquid. The method has the advantages of rapid phase split, integrated process and improves the concentration and purity of the flavones in the upper phase; the material consumption is reduced, the toxicity is small, the ethanol and the ammonium sulfate are easy to be recovered and utilized; the recovery rate of the flavones in the cedrela sinensis leaves is high, the production method is simple, easy to master and not affected by the environment; the cost is low, and the method is applicable to industrial production.

Description

technical field [0001] The invention belongs to a method for enriching and separating flavonoids from Chinese toon leaves in a two-phase aqueous system. Background technique [0002] Chinese toon [Toona sinensis (A.Juss.) Roem], also known as spring bud tree, Chinese toon, Chinese toon tree, is called red toon in "Plant Names and Facts", which belongs to the deciduous tree of the Meliaceae family (Meliaceae). Unique and precious fast-growing wood, vegetable and medicinal multi-purpose tree species, deciduous trees with huge crowns, luxuriant leaves, straight trunks, and rapid growth. Toona sinensis has very high medicinal value. It is pointed out in "Compendium of Materia Medica" that the leaves, buds, roots, bark and fruits of Toona sinensis can be used as medicine. Modern medical experimental studies have shown that Toona sinensis leaf extract has the functions of regulating lipid metabolism, alleviating hyperglycemia, improving sperm motility, delaying liver fibrosis, in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/58A61P1/16A61P3/06A61P3/10A61P15/08A61P35/00A61K127/00
Inventor 陈丛瑾黄克瀛
Owner GUANGXI UNIV
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