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Tissue culture breeding method of hippeastrum rutilum

A technology of tissue culture and culture medium, applied in the field of plant tissue culture, can solve the problems of low efficiency of Hippeastrum tissue culture and breeding, achieve the effect of solving vitrification, promoting the growth of bulbs, and improving economic benefits

Active Publication Date: 2021-08-13
GUANGDONG ACAD OF FORESTRY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Based on this, it is necessary to provide a method for tissue culture breeding of Hippeastrum to solve the problem of low efficiency of traditional Hippeastrum tissue culture

Method used

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  • Tissue culture breeding method of hippeastrum rutilum
  • Tissue culture breeding method of hippeastrum rutilum
  • Tissue culture breeding method of hippeastrum rutilum

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Experimental program
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Embodiment 1

[0068] Embodiment 1: the establishment of Zhudinglan tissue culture system ( figure 1 ) implementation steps:

[0069] 1. Explant preparation:

[0070] Use healthy and disease-free seed bulbs with a diameter of about 5cm as the material, use a fine brush to remove the soil on the surface of the material, cut off the root system, dip in detergent to clean, rinse with running water for 30 minutes, dry the water with filter paper and place it on an ultra-clean workbench for later use . Before inoculation, soak with 75% alcohol for 30s, rinse with sterile water for 3 times, then disinfect with 0.1% mercuric chloride for 8min, and rinse with sterile water for 3 times. Cut off about 1 / 2 of the upper part of the bulb bulb with a sterilized scalpel, peel off the outer layer of 1-3 layers of wrinkled and unfulfilled scales, and then divide the bulbs along the radial direction of the bulb, each group is at least 1cm wide , and must have a bulb disc at the base.

[0071] 2. Adventiti...

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Abstract

The invention discloses a tissue culture breeding method for hippeastrum hippeastrum. The method comprises the following steps: (a) supply of explants: taking scales with bulb discs at the bases as the explants; (b) adventitious bud induction: transferring the disinfected explants into an induction culture medium, and culturing the explants for 25-30 days to obtain adventitious bud seedlings; (c) multiplication culture: c1, carrying out subculture on the adventitious bud seedlings in a solid culture medium; c2, after culturing for 3-4 weeks, cutting seed balls obtained in the step c1, and transferring cut bodies into a semi-solid culture medium for culturing; c3, after culturing for 3-4 weeks, cutting the seed balls obtained in the step c2, and transferring cut bodies into the solid culture medium; wherein the solid culture medium is prepared by adding 2.0 mg / L to 2.5 mg / L of 6-BA, 25 g / L to 35 g / L of cane sugar and 5.5 g / L to 6.5 g / L of carrageenan into an MS (Murashige and Skoog) culture medium, and the semi-solid culture medium is prepared by adding 0.8 mg / L to 1.2 mg / L of 6-BA, 25 g / L to 35 g / L of cane sugar and 2.5 g / L to 3.5 g / L of carrageenan into a 1 / 2 MS culture medium; and (d) transplanting of seedlings: transplanting the seedlings obtained by multiplication culture into a matrix for culture.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for tissue culture and breeding of Hippeastrum chinensis. Background technique [0002] Hippeastrum rutilum, also known as amaryllis, huazhoulan, etc., is a perennial herb of the genus Hippeastrum in the family Amaryllidaceae. It is native to Peru and Brazil. It has tall and straight flower stems, large flowers, gorgeous colors, and high ornamental value. , deeply loved by consumers at home and abroad. Relevant studies have shown that Hippeastrum and its isolated chemical components have antibacterial, antiviral and antitumor effects, and have rich pharmacological effects. [0003] The commonly used propagation methods of Hippeastrum mainly include natural division, cutting and sowing, etc., but the propagation speed is slow and it is difficult to meet the market demand. The use of tissue culture technology for in vitro rapid propagation has the advantages...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001Y02A40/22
Inventor 廖焕琴杨会肖杨晓慧徐放陈新宇潘文朱报著徐斌张卫华
Owner GUANGDONG ACAD OF FORESTRY
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