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Method of Tissue Culture Breeding of Hippeastrum

A technology of tissue culture and culture medium, applied in the field of plant tissue culture, can solve the problems of low efficiency of Hippeastrum tissue culture and breeding, achieve the effect of solving vitrification, promoting the growth of bulbs, and improving economic benefits

Active Publication Date: 2022-06-07
GUANGDONG ACAD OF FORESTRY
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  • Summary
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Based on this, it is necessary to provide a method for tissue culture breeding of Hippeastrum to solve the problem of low efficiency of traditional Hippeastrum tissue culture

Method used

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  • Method of Tissue Culture Breeding of Hippeastrum
  • Method of Tissue Culture Breeding of Hippeastrum
  • Method of Tissue Culture Breeding of Hippeastrum

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0068] Example 1: Establishment of Zhu Dinglan tissue culture system ( Figure 1 Implementation steps:

[0069] 1. Explant preparation:

[0070] Using a robust and disease-free seed ball with a diameter of about 5 cm as the material, use a fine brush to remove the soil on the surface of the material, cut off the root system, dip the detergent to clean, rinse with running water for 30min, and use filter paper to absorb the water and place it on the ultra-clean workbench for later. Soak in 75% alcohol for 30s before inoculation, rinse 3 times with sterile water, then sterilize with 0.1% liters of mercury for 8 min and rinse 3 times with sterile water. About 1 / 2 of the upper part of the bulb is cut off with a sterilized scalpel, peeling off the outer layers of 1-3 layers of wrinkled, underfilled scales, and then dividing the bulbs radially along the bulb, each group is at least 1 cm wide, and the base must have a bulb disc.

[0071] 2. Adventitious bud induction:

[0072] MS+4.0mg / L6-...

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Abstract

The invention discloses a method for tissue culture and breeding of Hippeastrum, which comprises the following steps: (a) providing explants: using scales with bulb discs at the base as explants; (b) inducing adventitious buds: using the sterilized The explants are transferred to the induction medium, and cultured for 25 to 30 days to obtain adventitious sprouts; (c) Proliferation culture: c1, subculture the adventitious sprouts in a solid medium; c2, culture 3~ After 4 weeks, the bulbs obtained in step c1 are cut, and the cut bodies are transferred to a semi-solid medium for cultivation; c3, after 3 to 4 weeks of cultivation, the bulbs obtained in step c2 are cut, and the cut bodies are transferred to the (d) transplanting into seedlings: transplanting the seedlings obtained by the proliferation culture into the substrate for cultivation.

Description

Technical field [0001] The present invention relates to the field of plant tissue culture technology, specifically to a vermilion tissue culture breeding method. Background [0002] Hippeastrum rutilum, alias solitary flower, Orchid, etc., is a perennial herb of the genus Hippeastrum of the amaryllidaceae family, native to Brazil, Peru, with upright flower stems, large flowers, gorgeous flowers, high ornamental value, loved by consumers at home and abroad. Relevant studies have shown that zhudinghong and its isolated chemical components have antibacterial, antiviral and anti-tumor effects, and are rich in pharmacological effects. [0003] The commonly used propagation methods of vermilion are mainly natural splitting, cuttings and sowing, but the propagation speed is slow and it is difficult to meet the needs of the market. The use of tissue culture technology for in vitro fast propagation has the advantages of high propagation coefficient, short seedling formation time and conve...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001Y02A40/22
Inventor 廖焕琴杨会肖杨晓慧徐放陈新宇潘文朱报著徐斌张卫华
Owner GUANGDONG ACAD OF FORESTRY
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