Primary fir wood culture bud induction method

A technology of primary culture and bud induction, applied in the field of fir asexual reproduction, can solve problems such as vitrification and browning of explants, and achieve the effects of preventing browning, promoting bud induction, and good bud growth.

Active Publication Date: 2015-04-29
GUANGXI FORESTRY RES INST
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The object of the present invention is to provide a kind of fir primary culture bud induction method, improve the existing bud induction technology, overcome the problems such as explant browning and vitrification in the bud induction process, improve bud induction rate, bud index and bud growth status, Obtain a large number of buds with good quality, so as to meet the needs of multiplication and culture, and promote the establishment of a rapid propagation system of Chinese fir tissue culture

Method used

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  • Primary fir wood culture bud induction method
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  • Primary fir wood culture bud induction method

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Embodiment 1

[0026] A bud induction method for the primary generation of Chinese fir culture, the bud induction medium used is an improved 1 / 2 MS medium, adding kinetin 6-furfuryl aminopurine (KT), vitamin C, agar and sucrose; using 1 / 2 MS medium As the basic medium, NH in macroelements 4 NO 3 content of 550 mg / L; kinetin 6-furfurylaminopurine (KT) content of 1.0 mg / L; vitamin C content of 10 mg / L; agar content of 0.8 % and sucrose content of 3 % to make bud induction medium spare. Select the 4-6 cm long semi-lignified thick shoots that germinated from the base of Chinese fir as explants, cut off the needles and leaves of the shoots of Chinese fir, put them in a triangular flask, and add a concentration of 1-2% detergent For fine solution, seal the mouth of the bottle with gauze, wash it on a shaker with a rotating speed of 200 r / min for 10 min, then wash it under running water for 1 h, then wash it twice with sterile water, and turn the explants to ultra-clean Sterilize with 0.15% merc...

Embodiment 2

[0028] A bud induction method for primary culture of Chinese fir, the bud induction medium used is an improved 1 / 2MS medium, added with kinetin 6-furfurylaminopurine (KT), vitamin C, agar and sucrose; based on 1 / 2MS medium Medium, NH in macroelements 4 NO 3 content of 550 mg / L; kinetin 6-furfurylaminopurine (KT) content of 1.5 mg / L; vitamin C content of 15 mg / L; agar content of 0.8% and sucrose content of 3% to make bud induction medium spare. Select the thick semi-lignified shoots with a length of 4-6 cm germinated from the base of Chinese fir as explants, cut off the needles and leaves of the shoots of Chinese fir, place them in a triangular bottle, and add detergent with a concentration of 2-3% Solution, seal the bottle mouth with gauze, wash it on a shaking table with a rotation speed of 200r / min for 15min, then wash it under running water for 1.5h, then wash it twice with sterile water, and transfer the explant to the ultra-clean workbench Sterilize with 0.15% mercuric...

Embodiment 3

[0030] A bud induction method for primary culture of fir, the bud induction medium used is an improved 2 / 3MS medium, adding kinetin 6-furfuryl aminopurine (KT), vitamin C, agar and sucrose; based on 2 / 3MS medium Medium, NH in macroelements 4 NO 3 content of 825 mg / L; kinetin 6-furfurylaminopurine (KT) content of 2.0 mg / L; vitamin C content of 15 mg / L; agar content of 0.8% and sucrose content of 3%, making bud induction medium spare. Select 6-8 cm thick semi-lignified shoots that germinated from the base of Chinese fir as explants, cut off the needles and leaves of the shoots of Chinese fir, place them in a triangular flask, and add detergent with a concentration of 2-3%. For the fine solution, seal the bottle mouth with gauze, wash it on a shaking table with a rotation speed of 200r / min for 15min, then wash it under running water for 2h, then wash it twice with sterile water, and transfer the explant to the ultra-clean workbench Sterilize with 0.15% mercuric acid solution f...

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Abstract

The invention discloses a primary fir wood culture bud induction method. The method comprises the following steps: inoculating an explant as a coppice shoot of a fir wood base part and subjected to sterile explant treatment to a bud induction culture medium subjected to high-quality primary culture, culturing under the conditions of proper temperature, humidity and illumination intensity, and inducing bud germination. The conventional fir wood induction technology is improved, the problems that the explant is subjected to browning and vitrifaction in the fir wood bud induction process and the like are solved, the fir wood bud induction rate, the budding index and the germination and growth conditions are improved, and large-number high-quality buds are obtained, so that the multiplication culture requirement is met, the establishment of a rapid fir wood tissue culture reproductive system is promoted, and the method has high economic benefits and social benefits.

Description

technical field [0001] The invention relates to asexual propagation technology of Chinese fir, in particular to a method for inducing buds of the first generation of Chinese fir. Background technique [0002] Chinese fir( Cunninghamia lanceolata ) is a fir family plant, which is an important fast-growing timber tree species endemic to my country. Chinese fir has wide adaptability, excellent material and wide range of uses. It is one of the main tree species for afforestation in the southern regions of my country. With the development of my country's economy and the country's emphasis on forestry, the afforestation area of ​​Chinese fir will continue to expand, and the requirements for the quantity and quality of seedlings will continue to increase. market demand. Tissue culture technology is a rapid asexual reproduction technology. Select excellent Chinese fir families or clones as materials, and propagate seedlings through tissue culture methods, which can not only meet t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 黄开勇陈琴郝海坤戴俊张照远蓝肖唐庆兰
Owner GUANGXI FORESTRY RES INST
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