An initial culture bud inducing method for lithocarpus carolinae

A technology of primary culture and bud induction, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of browning of explants, improve germination rate, reduce pollution rate, and promote growth rate and robustness Effect

Inactive Publication Date: 2017-05-10
LIUZHOU LINGTONG TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a method that can overcome the problems of explant browning and vitrification in the bud induction process, improve the bud induction rate, bud index and bud growth status, and obtai

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The semi-lignified shoots germinated from the base of Cordyceps chinensis, with a length of 6-7 cm and a diameter of 0.2-0.3 cm were selected as explants. Cut off the needles and leaves of Hongxin Kemengtiao, put them in a triangular flask, add a detergent solution with a volume concentration of 1%, seal the bottle mouth with gauze, and wash it for 10 minutes on a shaker with a rotating speed of 200 r / min. Rinse under running water for 2 h, then wash twice with sterile water, transfer the explants to an ultra-clean workbench and sterilize them with 0.1% mercuric chloride solution for 10 min; then rinse with sterile water for 8~ After 10 times, the stems cut to 3.5 cm long were inoculated in the bud induction medium, and 2 stems were inoculated in each bottle.

[0022] The raw material content of the bud induction medium is: 1 / 3 improved WPM medium+6-BA 1.0mg / L+activated carbon 10mg / L+lactose 5.0g / L+sucrose 20.0g / L. Wherein the improved WPM medium composition and volume...

Embodiment 2

[0025] The semi-lignified sprouts with a length of 7-8 cm and a diameter of 0.2-0.3 cm that germinated at the base of Cordyceps chinensis were selected as explants. Cut off the needles and leaves of Hongxin Kemengtiao, put them in a triangular flask, add a 2% volume concentration of detergent solution, seal the bottle mouth with gauze, and wash it for 10 minutes on a shaker with a rotation speed of 200 r / min. Rinse under running water for 1.5 h, then wash twice with sterile water, transfer the explants to an ultra-clean workbench and sterilize them with 0.1% mercury chloride solution for 10 min; then rinse with sterile water for 8~ After 10 times, the stems cut to 3.5 cm long were inoculated in the bud induction medium, and 2 stems were inoculated in each bottle.

[0026]The raw material content of the bud induction medium is: 1 / 3 improved WPM medium+6-BA 1.5mg / L+activated carbon 10mg / L+lactose 5.0g / L+sucrose 20.0g / L. Wherein the improved WPM medium composition and volume to ...

Embodiment 3

[0029] The semi-lignified sprouts with a length of 7-8 cm and a diameter of 0.3-0.4 cm that germinated from the base of Cordyceps chinensis were selected as explants. Cut off the needles and leaves of Hongxin Kemengtiao, put them in a triangular flask, add a solution of detergent with a volume concentration of 4%, seal the mouth of the bottle with gauze, and wash it for 15 minutes on a shaker with a rotation speed of 200 r / min. Rinse under running water for 1 h, then wash twice with sterile water, transfer the explants to an ultra-clean workbench and sterilize them with 0.1% mercury chloride solution for 10 min; then rinse with sterile water for 8~ After 10 times, the stem segments cut to a length of 4.0 cm were inoculated in the bud induction medium, and 2 stem segments were inoculated in each bottle.

[0030] The raw material content of the bud induction medium is: 1 / 2 improved WPM medium+6-BA 1.5mg / L+activated carbon 10mg / L+lactose 5.0g / L+sucrose 20.0g / L. Wherein the impro...

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Abstract

An initial culture bud inducing method for lithocarpus carolinae is disclosed. The method is characterized in that lithocarpus carolinae root coppice shoots are adopted as explants, and the explants are subjected to aseptic processing, then inoculated to a high-quality initial culture bud inducing medium, and cultured under conditions including a proper temperature, proper humidity and proper illumination intensity to induce bud germination. The method overcomes problems such as explant browning and vitrification in a bud inducing process, increases the bud inducing rate, the budding index and bud growth statuses, and can obtain a high number of high-quality buds, thus meeting requirements of multiplication culture and promoting establishment of a tissue culture rapid multiplication system for the lithocarpus carolinae.

Description

technical field [0001] The invention relates to the asexual reproduction technology of Akesia chinensis, in particular to a method for inducing buds cultured in the first generation of Akesia chinensis. Background technique [0002] Lithocarpus carolinae (Skan) Rehd is an arbor of the genus Lithocarpus (Fagaceae), about 20 meters high, 40 cm in diameter at breast height, and the top bud scales are triangular and long lanceolate. Dark brown after drying, biennial branches have scattered lenticels, branches, bud scales, and leaves are all glabrous. Leaves thick papery, oblong, sparsely obovate-oblong, 13-18 cm long, 4-6 cm wide, apex caudate shortly pointed, base wedge-pointed, midrib slightly raised on leaf surface, or upper segment flat , 15-20 on each side of the lateral veins, reaching to the tooth end of the leaf margin, or sharply curved upward near the leaf margin, gradually slender and submerged, with stellate trichomes in the vein axils, the lower part of the leaf ma...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 黄文卫
Owner LIUZHOU LINGTONG TECH CO LTD
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