An initial culture bud inducing method for lithocarpus carolinae
A technology of primary culture and bud induction, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of browning of explants, improve germination rate, reduce pollution rate, and promote growth rate and robustness Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0021] The semi-lignified shoots germinated from the base of Cordyceps chinensis, with a length of 6-7 cm and a diameter of 0.2-0.3 cm were selected as explants. Cut off the needles and leaves of Hongxin Kemengtiao, put them in a triangular flask, add a detergent solution with a volume concentration of 1%, seal the bottle mouth with gauze, and wash it for 10 minutes on a shaker with a rotating speed of 200 r / min. Rinse under running water for 2 h, then wash twice with sterile water, transfer the explants to an ultra-clean workbench and sterilize them with 0.1% mercuric chloride solution for 10 min; then rinse with sterile water for 8~ After 10 times, the stems cut to 3.5 cm long were inoculated in the bud induction medium, and 2 stems were inoculated in each bottle.
[0022] The raw material content of the bud induction medium is: 1 / 3 improved WPM medium+6-BA 1.0mg / L+activated carbon 10mg / L+lactose 5.0g / L+sucrose 20.0g / L. Wherein the improved WPM medium composition and volume...
Embodiment 2
[0025] The semi-lignified sprouts with a length of 7-8 cm and a diameter of 0.2-0.3 cm that germinated at the base of Cordyceps chinensis were selected as explants. Cut off the needles and leaves of Hongxin Kemengtiao, put them in a triangular flask, add a 2% volume concentration of detergent solution, seal the bottle mouth with gauze, and wash it for 10 minutes on a shaker with a rotation speed of 200 r / min. Rinse under running water for 1.5 h, then wash twice with sterile water, transfer the explants to an ultra-clean workbench and sterilize them with 0.1% mercury chloride solution for 10 min; then rinse with sterile water for 8~ After 10 times, the stems cut to 3.5 cm long were inoculated in the bud induction medium, and 2 stems were inoculated in each bottle.
[0026]The raw material content of the bud induction medium is: 1 / 3 improved WPM medium+6-BA 1.5mg / L+activated carbon 10mg / L+lactose 5.0g / L+sucrose 20.0g / L. Wherein the improved WPM medium composition and volume to ...
Embodiment 3
[0029] The semi-lignified sprouts with a length of 7-8 cm and a diameter of 0.3-0.4 cm that germinated from the base of Cordyceps chinensis were selected as explants. Cut off the needles and leaves of Hongxin Kemengtiao, put them in a triangular flask, add a solution of detergent with a volume concentration of 4%, seal the mouth of the bottle with gauze, and wash it for 15 minutes on a shaker with a rotation speed of 200 r / min. Rinse under running water for 1 h, then wash twice with sterile water, transfer the explants to an ultra-clean workbench and sterilize them with 0.1% mercury chloride solution for 10 min; then rinse with sterile water for 8~ After 10 times, the stem segments cut to a length of 4.0 cm were inoculated in the bud induction medium, and 2 stem segments were inoculated in each bottle.
[0030] The raw material content of the bud induction medium is: 1 / 2 improved WPM medium+6-BA 1.5mg / L+activated carbon 10mg / L+lactose 5.0g / L+sucrose 20.0g / L. Wherein the impro...
PUM
Property | Measurement | Unit |
---|---|---|
Diameter | aaaaa | aaaaa |
Diameter | aaaaa | aaaaa |
Diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com