35results about How to "Strong meristem ability" patented technology

The invention provides a rapid rhizoma polygonati propagation technology method. The method comprises the following steps: breaking seed dormancy by changing temperature treatment and hormone treatment, performing induction in vitro to culture callus by utilizing seeds subjected to dormancy breakage as materials, performing differentiation culture and root induction, and performing vegetative propagation on rhizoma polygonati seedlings. By adoption of the technology, the rhizoma polygonati propagation factor can be greatly improved, the production period is shortened, the sowing quantity is reduced, and the production cost is reduced.

The invention discloses a primary fir wood culture bud induction method. The method comprises the following steps: inoculating an explant as a coppice shoot of a fir wood base part and subjected to sterile explant treatment to a bud induction culture medium subjected to high-quality primary culture, culturing under the conditions of proper temperature, humidity and illumination intensity, and inducing bud germination. The conventional fir wood induction technology is improved, the problems that the explant is subjected to browning and vitrifaction in the fir wood bud induction process and the like are solved, the fir wood bud induction rate, the budding index and the germination and growth conditions are improved, and large-number high-quality buds are obtained, so that the multiplication culture requirement is met, the establishment of a rapid fir wood tissue culture reproductive system is promoted, and the method has high economic benefits and social benefits.

The invention discloses a tissue culture rapid propagation seedling method of hybrid paper mulberry, and belongs to the technical field of hybrid paper mulberry plantation. The tissue culture rapid propagation seedling method of hybrid paper mulberry comprises following steps: explant selection, induced culturing, subculturing, rooting culture, and transplanting. According to the tissue culture rapid propagation seedling method, current year stems with buds are selected as explants, the meristematic capacity is excellent, and differentiation propagation is more convenient to realize; LED illumination culture is capable of adjusting culture material growth process, increasing the environmental adaptability, realizing reasonable optimization on composition of induction medium, proliferationmedium, and rooting medium, and illumination conditions of different stages, increasing seedling tissue culture efficiency, and achieving relatively induction rate, reproduction rate, and rooting rate; the adventitious bud induction differentiation rate is 94% or higher; after subculture, average seedling height reaches 3.5cm, rooting rate reaches 100%, and transplanting survival rate reaches 68%or higher. The tissue culture rapid propagation seedling method is adopted for seedling of hybrid paper mulberry, the seedling efficiency is high, the propagation coefficient is large, seedling cost is reduced, and the obtained tissue culture seedlings are strong, and are high in quality.

The invention relates to a tissue culture method of Pueraria thomsonii, comprising the steps of treating an explant, to be specific, collecting a Pueraria thomsonii twig with axillary buds, disinfecting, and killing bacteria for use; inducing a callus, to be specific, inducing the treated Pueraria thomsonii twig to a first medium for culturing to obtain the callus; inducing cluster buds, to be specific, dicing the callus, inoculating to a second medium, and culturing to obtain cluster buds; performing rooting culturing, to be specific, dividing the cluster buds to obtain single buds, transplanting the single buds to a third medium, and culturing to obtain Pueraria thomsonii plantlets. The tissue culture method of Pueraria thomsonii has the advantages that the Pueraria thomsonii twig with axillary buds having high differentiating capacity and low polyphenol content is used as an explant, different media are used in different culture stages, browning of Pueraria thomsonii during tissue culture is prevented, and survival rate of Pueraria thomsonii in tissue culture is effectively increased.

The invention discloses a snakegourd fruit tissue culture seedling growing method, and relates to the technical field of snakegourd fruit seedling growing. The method comprises the steps of explant selection, tissue seedling culture, nursery selection and transplantation and growing of tissue culture seedlings. According to the method, seedling growing is conducted through tissue culture, seedlinggrowing efficiency is improved greatly, seedling growing cost is reduced, and the varietal characteristics of the snakegourd fruit can be well maintained. Compared with a traditional root-divided breeding method, the reproduction coefficient can be increased by 4-5 times, the seedling growing period can be shortened by 10%, a snakegourd fruit rapid propagation technical system can be established,and seedling yield can be increased.

The invention relates to a tissue culture propagation method of Polygonatum multiflorum, comprising the steps of in an aseptic environment, collecting embryo from mature seeds of Polygonatum multiflorum by peeling, and sterilizing the embryo; inoculating the sterilized embryo to a callus induction medium, and performing light-free culture for 7-12 days so that the embryo expands to form a callus;cutting the callus into small pieces, inoculating the pieces to a callus subculture medium, and culturing for 18-25 days under light intensity of 800-1000 lux and daily lighting time of 10-12 h; cutting the callus subjected to subculture in step S3 into small pieces, inoculating the pieces to a sprouting medium, and culturing for 28-35 days under the light intensity of 1200-1800 lux and daily lighting time of 10-12 h, and inducing to obtain cluster buds; inoculating the robust single ones of the cluster buds to a rooting medium, and performing rooting culturing. The method herein helps greatlyreduce pollution rate of a tissue culture process, and improve propagation efficiency and seedling quality.

The invention discloses a cinnamomum micranthum cuttage breeding method which mainly includes the steps: cutting treatment; cutting bed treatment; seedbed management. Cutting treatment mainly relatesto cutting portions of cuttings, diameter classes of the cuttings, the lengths of the cuttings, cutting modes of bases of the cuttings, rooting treatment of the cuttings and the like. Cutting bed treatment mainly relates to selection of cutting bed media, cutting bed disinfection before cuttage, cutting modes, cutting bed treatment after cutting and the like. According to the method, the survivalrate of the cuttings of cinnamomum micranthum can reach 87% and is increased by 74% as compared with an existing traditional method.

The invention provides a cuttage cultivation method for picria fel-terrae lour. According to the method, the tip portion of the picria fel-terrae lour which is good in growth condition and free of the disease and pest damage is obtained and directly inserted in the soil in a cuttage mode, a callus is formed in the cuttage portion, then rooting is achieved and the plant of the picria fel-terrae lour is obtained. According to the method, a high survival rate and a high growth rate of cultivation of the picria fel-terrae lour can be guaranteed.

The invention discloses a method for inducing rhizoma bletillae tetraploid through calluses. A chemical inducer comprising 0.05%-0.2% of colchicines and 3% of DMSO is used for conducting short-time induction on the calluses, proliferation, differentiation and rooting culture are conducted, and the inductivity of the rhizoma bletillae tetraploid is 2.6%-23.4% through experimental statistics. According to the method, the inductivity of the tetraploid can be increased, and the toxic effect of the chemical inducer on cells can be relieved. Based on the cell totipotency principle, in the differentiation process, one cell is differentiated into a plant, and heterozygotes can be effectively reduced. Thus, the method has the advantages that operation is simple, control is easy, repeatability is good, inductivity is high, and the purification rate of polyploidy is high.

The invention discloses a primary culture bud inducing method of lithocarpus oleaefolius. The primary culture bud inducing method is characterized by being prepared by applying root coppice shoot of lithocarpus oleaefolius as an explant; after the asepsis of the explant, inoculating the explant to an inducing culture base of a high-quality primary culture bud; and culturing the explant under suitable temperature, humidity and light intensity, and inducing the bud germination. The method overcomes the problems of explant brown stain, vitrification and other problems during the bud inducing process, improves the bud inducing rate, budding rate and bud growth status, and acquires buds with large amount and high quality, and thereby meeting the demand of multiplication culture and promoting the setup of a rapid propagation system of the lithocarpus oleaefolius tissue culture.

The invention provides a hydroponic root induction nutrition solution suitable for agathis dammara. The nutrition solution comprises 0.5 mg/L naphthylacetic acid, 472 mg/L calcium nitrate tetrahydrate, 202 mg/L potassium nitrate, 80 mg/L ammonium nitrate, 100 mg/L potassium dihydrogen phosphate, 174 mg/L potassium sulfate and 246 mg/L magnesium sulfate heptahydrate. The prepared hydroponic root induction nutrition solution promotes the healthy and strong growth of hydroponic agathis dammara, and is low in root system rotting rate, high in hydroponic root induction rate and numerous and strongin hydroponic roots are.