35results about How to "Strong meristem ability" patented technology

The invention provides a rapid rhizoma polygonati propagation technology method. The method comprises the following steps: breaking seed dormancy by changing temperature treatment and hormone treatment, performing induction in vitro to culture callus by utilizing seeds subjected to dormancy breakage as materials, performing differentiation culture and root induction, and performing vegetative propagation on rhizoma polygonati seedlings. By adoption of the technology, the rhizoma polygonati propagation factor can be greatly improved, the production period is shortened, the sowing quantity is reduced, and the production cost is reduced.

The invention discloses a primary fir wood culture bud induction method. The method comprises the following steps: inoculating an explant as a coppice shoot of a fir wood base part and subjected to sterile explant treatment to a bud induction culture medium subjected to high-quality primary culture, culturing under the conditions of proper temperature, humidity and illumination intensity, and inducing bud germination. The conventional fir wood induction technology is improved, the problems that the explant is subjected to browning and vitrifaction in the fir wood bud induction process and the like are solved, the fir wood bud induction rate, the budding index and the germination and growth conditions are improved, and large-number high-quality buds are obtained, so that the multiplication culture requirement is met, the establishment of a rapid fir wood tissue culture reproductive system is promoted, and the method has high economic benefits and social benefits.

The invention discloses a tissue culture rapid propagation seedling method of hybrid paper mulberry, and belongs to the technical field of hybrid paper mulberry plantation. The tissue culture rapid propagation seedling method of hybrid paper mulberry comprises following steps: explant selection, induced culturing, subculturing, rooting culture, and transplanting. According to the tissue culture rapid propagation seedling method, current year stems with buds are selected as explants, the meristematic capacity is excellent, and differentiation propagation is more convenient to realize; LED illumination culture is capable of adjusting culture material growth process, increasing the environmental adaptability, realizing reasonable optimization on composition of induction medium, proliferationmedium, and rooting medium, and illumination conditions of different stages, increasing seedling tissue culture efficiency, and achieving relatively induction rate, reproduction rate, and rooting rate; the adventitious bud induction differentiation rate is 94% or higher; after subculture, average seedling height reaches 3.5cm, rooting rate reaches 100%, and transplanting survival rate reaches 68%or higher. The tissue culture rapid propagation seedling method is adopted for seedling of hybrid paper mulberry, the seedling efficiency is high, the propagation coefficient is large, seedling cost is reduced, and the obtained tissue culture seedlings are strong, and are high in quality.

The invention relates to a tissue culture method of Pueraria thomsonii, comprising the steps of treating an explant, to be specific, collecting a Pueraria thomsonii twig with axillary buds, disinfecting, and killing bacteria for use; inducing a callus, to be specific, inducing the treated Pueraria thomsonii twig to a first medium for culturing to obtain the callus; inducing cluster buds, to be specific, dicing the callus, inoculating to a second medium, and culturing to obtain cluster buds; performing rooting culturing, to be specific, dividing the cluster buds to obtain single buds, transplanting the single buds to a third medium, and culturing to obtain Pueraria thomsonii plantlets. The tissue culture method of Pueraria thomsonii has the advantages that the Pueraria thomsonii twig with axillary buds having high differentiating capacity and low polyphenol content is used as an explant, different media are used in different culture stages, browning of Pueraria thomsonii during tissue culture is prevented, and survival rate of Pueraria thomsonii in tissue culture is effectively increased.

The invention discloses a culture medium and a rapid propagation method for tissue culture of Vitex agnus-castus L, wherein the culture medium comprises a bud induction culture medium, a proliferationculture medium and a rooting culture medium. According to the present invention, by using the specific culture medium formula of the three components, the proliferation coefficient and the rooting rate of the tissue culture of Vitex agnus-castus L are significantly improved so as to improve the proliferation efficiency of Vitex agnus-castus L; the current growth branch of Vitex agnus-castus L isselected as the explant material, has characteristics of strong growth and strong branching ability, and does not easily produce browning during the tissue culture; and the reproductive efficiency ofVitex agnus-castus L is improved, and the culture medium and the method can meet the requirements of large-scale production.

The invention discloses a planting method of picria fel-terrae. The method includes the steps of firstly, cultivating reproduction materials; secondly, preparing soil and weeding; thirdly, planting; fourthly, field management; fifthly, harvesting. The method has the advantages that by the tuber division cultivation condition, soil requirements are low, planting cost is lowered, emergence rate of the picria fel-terrae is guaranteed, cultivated seedlings are robust, soil nutrition is sufficient, seedling growing states are good, grown seedlings are high in draught resistance and disease and pest resistance, fast in root growing speed and high in yield, and the method is especially suitable for large-scale planting, and worthy of popularization.

The invention discloses a snakegourd fruit tissue culture seedling growing method, and relates to the technical field of snakegourd fruit seedling growing. The method comprises the steps of explant selection, tissue seedling culture, nursery selection and transplantation and growing of tissue culture seedlings. According to the method, seedling growing is conducted through tissue culture, seedlinggrowing efficiency is improved greatly, seedling growing cost is reduced, and the varietal characteristics of the snakegourd fruit can be well maintained. Compared with a traditional root-divided breeding method, the reproduction coefficient can be increased by 4-5 times, the seedling growing period can be shortened by 10%, a snakegourd fruit rapid propagation technical system can be established,and seedling yield can be increased.

The invention relates to a tissue culture propagation method of Polygonatum multiflorum, comprising the steps of in an aseptic environment, collecting embryo from mature seeds of Polygonatum multiflorum by peeling, and sterilizing the embryo; inoculating the sterilized embryo to a callus induction medium, and performing light-free culture for 7-12 days so that the embryo expands to form a callus;cutting the callus into small pieces, inoculating the pieces to a callus subculture medium, and culturing for 18-25 days under light intensity of 800-1000 lux and daily lighting time of 10-12 h; cutting the callus subjected to subculture in step S3 into small pieces, inoculating the pieces to a sprouting medium, and culturing for 28-35 days under the light intensity of 1200-1800 lux and daily lighting time of 10-12 h, and inducing to obtain cluster buds; inoculating the robust single ones of the cluster buds to a rooting medium, and performing rooting culturing. The method herein helps greatlyreduce pollution rate of a tissue culture process, and improve propagation efficiency and seedling quality.

The invention discloses a cinnamomum micranthum cuttage breeding method which mainly includes the steps: cutting treatment; cutting bed treatment; seedbed management. Cutting treatment mainly relatesto cutting portions of cuttings, diameter classes of the cuttings, the lengths of the cuttings, cutting modes of bases of the cuttings, rooting treatment of the cuttings and the like. Cutting bed treatment mainly relates to selection of cutting bed media, cutting bed disinfection before cuttage, cutting modes, cutting bed treatment after cutting and the like. According to the method, the survivalrate of the cuttings of cinnamomum micranthum can reach 87% and is increased by 74% as compared with an existing traditional method.

The invention provides a cuttage cultivation method for picria fel-terrae lour. According to the method, the tip portion of the picria fel-terrae lour which is good in growth condition and free of the disease and pest damage is obtained and directly inserted in the soil in a cuttage mode, a callus is formed in the cuttage portion, then rooting is achieved and the plant of the picria fel-terrae lour is obtained. According to the method, a high survival rate and a high growth rate of cultivation of the picria fel-terrae lour can be guaranteed.

The invention provides a cultivation method for coleus salix holandia, and relates to the technical field of plant cultivation. The cultivation method comprises the following steps: yard treatment, cutting strip selection and treatment, cutting and final-period management. By the cultivation method for the coleus salix holandia, robust growth of the coleus salix holandia is promoted by deep tillage of soil and fertilizer application, stress resistance is improved, nutrients required for growth in a whole year is ensured fully, reasonable utilization of fertilizer efficiency is ensured by different fertilizer application modes in a growth period, the fertilizer utilization ratio is high, by pest control in the final-period management, diseases of the coleus salix holandia can be prevented and treated, and the rate of survival of the cutting strips is increased.

The invention relates to a root inducing and culturing technique for araucaria heterophylla during hydroponics transferred from earth culturing or substrate culturing, in particular to the technique including selection of araucaria heterophylla suitable for hydroponics, treatment of the roots of araucaria heterophylla before hydroponics, preparation of the root inducing nutrient solution in hydroponics and preparation of nutrient solution for the better growth of the araucaria hydroponics, and belongs to the technical field of woody plant hydroponics. By the root inducing and culturing techniques, the araucaria heterophylla is changed from earth culturing or substrate culturing to hydroponics, and preparation formula of root inducing and growing nutrient solution in hydroponics and the matching technique with low rate of root rotting, high rate of root inducing water, large amount of thick hydroponics roots and robust plants are provided. The root inducing and culturing technique for araucaria heterophylla hydroponics can be applied to the factory production of araucaria heterophylla soilless nutrient solution and home planting hydroponics.

The invention provides a cultivation method for early-fruiting and high-yield pear trees in the north, and relates to the technical field of fruit tree cultivation. The cultivation method comprises the steps of selection and treatment of cuttings, management in the later period and treatment after the pear trees grow for 1-2 years. The cultivation method is scientific, the peer trees fruit early and are high in yield, most diseases and pests attacking the pear trees easily can be prevented, the morbidity of the pear trees in the later period can be reduced, the management in the later period is facilitated, and the losses caused by the diseases and the pests are reduced; pears finally produced by means of the cultivation method are good in taste, high in quality, beautiful in color and high in sweetness, and the pears contain abundant selenium and are beneficial to health.

The invention discloses a method for inducing rhizoma bletillae tetraploid through calluses. A chemical inducer comprising 0.05%-0.2% of colchicines and 3% of DMSO is used for conducting short-time induction on the calluses, proliferation, differentiation and rooting culture are conducted, and the inductivity of the rhizoma bletillae tetraploid is 2.6%-23.4% through experimental statistics. According to the method, the inductivity of the tetraploid can be increased, and the toxic effect of the chemical inducer on cells can be relieved. Based on the cell totipotency principle, in the differentiation process, one cell is differentiated into a plant, and heterozygotes can be effectively reduced. Thus, the method has the advantages that operation is simple, control is easy, repeatability is good, inductivity is high, and the purification rate of polyploidy is high.

The invention discloses a primary fir wood culture bud induction method. The method comprises the following steps: inoculating an explant as a coppice shoot of a fir wood base part and subjected to sterile explant treatment to a bud induction culture medium subjected to high-quality primary culture, culturing under the conditions of proper temperature, humidity and illumination intensity, and inducing bud germination. The conventional fir wood induction technology is improved, the problems that the explant is subjected to browning and vitrifaction in the fir wood bud induction process and the like are solved, the fir wood bud induction rate, the budding index and the germination and growth conditions are improved, and large-number high-quality buds are obtained, so that the multiplication culture requirement is met, the establishment of a rapid fir wood tissue culture reproductive system is promoted, and the method has high economic benefits and social benefits.

The invention discloses a culture method of cunninghamia lanceolata callus. The culture method comprises the steps of: screening cunninghamia lanceolata seeds with filled particles and without pests as explants, carrying out sterile culture to obtain sterile seedlings, clipping leaves, stems or root systems of the sterile seedlings, inoculating in an induction culture medium for inducing callusogenesis, and finally transferring the callus formed by induction into a proliferation medium for proliferation. The culture method is simple and easy to operate, is capable of effectively shortening the induction and proliferation time of the callus and increasing the induction rate of the cunninghamia lanceolata callus, provides a large quantity of excellent callus materials with good colors and compact structures for the development of the cunninghamia lanceolata industry, and has a good economic benefit.

The invention discloses a primary culture bud inducing method of lithocarpus oleaefolius. The primary culture bud inducing method is characterized by being prepared by applying root coppice shoot of lithocarpus oleaefolius as an explant; after the asepsis of the explant, inoculating the explant to an inducing culture base of a high-quality primary culture bud; and culturing the explant under suitable temperature, humidity and light intensity, and inducing the bud germination. The method overcomes the problems of explant brown stain, vitrification and other problems during the bud inducing process, improves the bud inducing rate, budding rate and bud growth status, and acquires buds with large amount and high quality, and thereby meeting the demand of multiplication culture and promoting the setup of a rapid propagation system of the lithocarpus oleaefolius tissue culture.

The invention discloses a primary culture bud induction method for lithocarpus amygdalifolius. The method is characterized by comprising the following steps: taking a base coppice shoot of the lithocarpus amygdalifolius as an explant, performing aseptic treatment on the explant, inoculating the explant to a high-quality primary culture bud induction medium, culturing under the conditions of appropriate temperature, humidity and illumination intensity, and inducing bud germination. According to the method disclosed by the invention, the problems such as explant browning, vitrification and the like in the bud induction process are solved, the bud induction rate, budding index and germination and growth conditions are improved, and a great number of high-quality buds are obtained, so that the multiplication culture requirements are met, and establishment of a rapid tissue culture propagation system for the lithocarpus amygdalifolius is facilitated.

The invention provides a hydroponic root induction nutrition solution suitable for agathis dammara. The nutrition solution comprises 0.5 mg / L naphthylacetic acid, 472 mg / L calcium nitrate tetrahydrate, 202 mg / L potassium nitrate, 80 mg / L ammonium nitrate, 100 mg / L potassium dihydrogen phosphate, 174 mg / L potassium sulfate and 246 mg / L magnesium sulfate heptahydrate. The prepared hydroponic root induction nutrition solution promotes the healthy and strong growth of hydroponic agathis dammara, and is low in root system rotting rate, high in hydroponic root induction rate and numerous and strongin hydroponic roots are.

The invention relates to the field of plant culture and particularly relates to a culturing method of a polyploid anoectochilus roxburghii variety. The culturing method comprises the following steps of culturing a diploid anoectochilus roxburghii seed to obtain a protocorm; soaking the protocorm in 5-15mu mol / L of an oryzalin solution to treat for 10-36 hours; further culturing the soaked protocorm to obtain a clustered shoot; after dividing, transferring the clustered shoot to a rooting medium to culture to obtain a tissue culture seedling with complete root, stem and leaves; and sieving the tissue culture seedling to obtain a polyploid plant. According to the culturing method of the polyploid anoectochilus roxburghii variety, provided by the invention, the anoectochilus roxburghii protocorm with very strong dividing capacity is used as an inducing material, the protocorm is soaked for the specific time by using the oryzalin solution with the specific concentration, and then, a polyploid anoectochilus roxburghii plant can be obtained through screening in cultured plants, wherein the induction rate ranges from 43.2% to 44.8%, and the obtained polyploid anoectochilus roxburghii plant is favorable in character.

The invention relates to a tissue culture propagation method of Polygonatum multiflorum, comprising the steps of in an aseptic environment, collecting embryo from mature seeds of Polygonatum multiflorum by peeling, and sterilizing the embryo; inoculating the sterilized embryo to a callus induction medium, and performing light-free culture for 7-12 days so that the embryo expands to form a callus;cutting the callus into small pieces, inoculating the pieces to a callus subculture medium, and culturing for 18-25 days under light intensity of 800-1000 lux and daily lighting time of 10-12 h; cutting the callus subjected to subculture in step S3 into small pieces, inoculating the pieces to a sprouting medium, and culturing for 28-35 days under the light intensity of 1200-1800 lux and daily lighting time of 10-12 h, and inducing to obtain cluster buds; inoculating the robust single ones of the cluster buds to a rooting medium, and performing rooting culturing. The method herein helps greatlyreduce pollution rate of a tissue culture process, and improve propagation efficiency and seedling quality.

The invention relates to a tissue culture method of Pueraria thomsonii, comprising the steps of treating an explant, to be specific, collecting a Pueraria thomsonii twig with axillary buds, disinfecting, and killing bacteria for use; inducing a callus, to be specific, inducing the treated Pueraria thomsonii twig to a first medium for culturing to obtain the callus; inducing cluster buds, to be specific, dicing the callus, inoculating to a second medium, and culturing to obtain cluster buds; performing rooting culturing, to be specific, dividing the cluster buds to obtain single buds, transplanting the single buds to a third medium, and culturing to obtain Pueraria thomsonii plantlets. The tissue culture method of Pueraria thomsonii has the advantages that the Pueraria thomsonii twig with axillary buds having high differentiating capacity and low polyphenol content is used as an explant, different media are used in different culture stages, browning of Pueraria thomsonii during tissue culture is prevented, and survival rate of Pueraria thomsonii in tissue culture is effectively increased.

The invention provides a cuttage cultivation method for picria fel-terrae lour. According to the method, the tip portion of the picria fel-terrae lour which is good in growth condition and free of the disease and pest damage is obtained and directly inserted in the soil in a cuttage mode, a callus is formed in the cuttage portion, then rooting is achieved and the plant of the picria fel-terrae lour is obtained. According to the method, a high survival rate and a high growth rate of cultivation of the picria fel-terrae lour can be guaranteed.

The invention discloses a culture medium and a rapid propagation method for tissue culture of Vitex agnus-castus L, wherein the culture medium comprises a bud induction culture medium, a proliferationculture medium and a rooting culture medium. According to the present invention, by using the specific culture medium formula of the three components, the proliferation coefficient and the rooting rate of the tissue culture of Vitex agnus-castus L are significantly improved so as to improve the proliferation efficiency of Vitex agnus-castus L; the current growth branch of Vitex agnus-castus L isselected as the explant material, has characteristics of strong growth and strong branching ability, and does not easily produce browning during the tissue culture; and the reproductive efficiency ofVitex agnus-castus L is improved, and the culture medium and the method can meet the requirements of large-scale production.

The invention provides a rapid rhizoma polygonati propagation technology method. The method comprises the following steps: breaking seed dormancy by changing temperature treatment and hormone treatment, performing induction in vitro to culture callus by utilizing seeds subjected to dormancy breakage as materials, performing differentiation culture and root induction, and performing vegetative propagation on rhizoma polygonati seedlings. By adoption of the technology, the rhizoma polygonati propagation factor can be greatly improved, the production period is shortened, the sowing quantity is reduced, and the production cost is reduced.

The present invention relates to a rapid lotus corniculatus l. breeding method, which comprises the following steps: 1) sterilizing lotus corniculatus l. seeds; 2) placing the sterilized and washed lotus corniculatus l.seeds in a culture dish under a sterile state, and culturing for 3-5 days in an illumination and moisture environment to germinate; 3) transferring the germinating seeds obtained from the step 2) in a hormone-free MS base culture medium, wherein a room temperature is 25-27 DEG C, light intensity is 2000 lux, an illumination time every day is 12 h, and a culture time is 20-25 days; 4) taking the bud after a length of the bud is 10-12 cm, and cutting under a sterile condition, wherein a length of every stem segment is about 1.5-2 cm, and has a mature leaf; 5) transferring the stem segment into a disposable growth culture medium; and 6) after cutting the stem segment, placing in a certain environment, wherein a room temperature is 25-27 DEG C, light intensity is 2000-3000 lux, an illumination time every day is 12 h, and a culture time is 20-25 days. With the present invention, a culture period is short, operation management and application promotion are easily achieved, production cost can be significantly reduced, transplantation survival rate is significantly increased, and vitreous shoot phenomenon can be completely eliminated.