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Rapid lotus corniculatus l. breeding method

A fast technology of L. japonicus, which is applied in the field of fast propagation of L. japonicus, can solve the problems affecting the normal development and growth of the root system, and achieve good growth of the seedling root system, strong growth of the plant and root system, and strong meristemality. Effect

Inactive Publication Date: 2014-07-30
SHENZHEN NONGKE GROUP +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The one-time rooting and seedling cultivation technology has the characteristics of short cultivation period, simple facilities, low cost, and easy promotion. The incision is very easy to form irregular callus and cell mass, which greatly affects the normal development and growth of the root system, which has become a major problem to be solved by this technology

Method used

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  • Rapid lotus corniculatus l. breeding method
  • Rapid lotus corniculatus l. breeding method
  • Rapid lotus corniculatus l. breeding method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Disinfect 0.5-1 g (1,000-grain weight 1-1.2 g) mature plump seeds of japonicus japonicus with 0.1% mercuric chloride solution for 20 minutes, and rinse them with sterile water; In the petri dish of filter paper, germinate under light for 5 days; transfer the germinated seeds to the MS basic medium without hormones, room temperature 25-27 ℃, light intensity 2000lux, 12h light time per day, and cultivate for 25 days; Take out when it grows to 10-12cm in length, cut into sections under sterile conditions, each stem section is about 1.5cm long, with 1 mature leaf; transfer the stem section into a bottle containing disposable growth medium, and cut 20 stems in each bottle Segment; After cutting the stem segment, place the bottle in a room temperature of 25-27°C, 2000 lux, and 12 hours of light per day for cultivation. After 25 days, the bottle can be transplanted or subcultured.

[0029] Among them, the one-time growth medium formula is the complete nutrient composition of M...

Embodiment 2

[0031] 0.5-1 gram (dry weight 1-1.2 grams) of japonicus japonicus mature plump seeds are sterilized with 0.1% mercuric chloride for 20 minutes, and rinsed with sterile water; the rinsed japonicus japonicus seeds are placed in a sterile state containing In a petri dish with wet filter paper, germinate under light for 3-5 days; transfer the germinated seeds to MS basic medium without hormones, at room temperature 25-27°C, light intensity 2000lux, 12h light per day, and culture for 20-20 25 days; take out the buds when they grow to 10-12cm in length, cut into sections under sterile conditions, each stem section is about 2cm long, with 1 mature leaf; transfer the stem section into a bottle containing a disposable growth medium, each bottle Cut 20 stem segments; after the stem segments are cut, place the bottle in a room temperature of 25-27°C, 3000 lux, and 12 hours of light per day for cultivation. After 20 days, the bottle can be transplanted or subcultured.

[0032] Among them,...

Embodiment 3

[0034] In recent years, many batches of japonicus japonicus plants have been propagated with this technology. Through field planting, multiple variety transplantation comparison tests have been carried out with conventional seedlings (control). Planting, the growth period is three months, and the result is that the survival rate of clone transplantation is increased by about 10% to 15% compared with the control, and the plant grows strongly and grows fast. During the 4 years from 2007 to 2010, the fresh weight of the stems and leaves of the japonicus plants planted in autumn and grown in the field for 60 days were measured in November every year. 100 treated plants were measured each time, and 100 seeds were sown at the same time. Seedling plant is as contrast, measures hundreds of stems and leaves fresh weight and gets average value (average single plant fresh weight=100 stems and leaves total fresh weight (gram) / 100), measures continuously 4 years, and the result shows, the c...

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Abstract

The present invention relates to a rapid lotus corniculatus l. breeding method, which comprises the following steps: 1) sterilizing lotus corniculatus l. seeds; 2) placing the sterilized and washed lotus corniculatus l.seeds in a culture dish under a sterile state, and culturing for 3-5 days in an illumination and moisture environment to germinate; 3) transferring the germinating seeds obtained from the step 2) in a hormone-free MS base culture medium, wherein a room temperature is 25-27 DEG C, light intensity is 2000 lux, an illumination time every day is 12 h, and a culture time is 20-25 days; 4) taking the bud after a length of the bud is 10-12 cm, and cutting under a sterile condition, wherein a length of every stem segment is about 1.5-2 cm, and has a mature leaf; 5) transferring the stem segment into a disposable growth culture medium; and 6) after cutting the stem segment, placing in a certain environment, wherein a room temperature is 25-27 DEG C, light intensity is 2000-3000 lux, an illumination time every day is 12 h, and a culture time is 20-25 days. With the present invention, a culture period is short, operation management and application promotion are easily achieved, production cost can be significantly reduced, transplantation survival rate is significantly increased, and vitreous shoot phenomenon can be completely eliminated.

Description

technical field [0001] The invention relates to the technical field of plant vegetative propagation, in particular to a method for rapid propagation of japonicus japonicus. Background technique [0002] Lotus cornioulatus L.), also known as bird foot bean, barren sativa, is a perennial herb of the leguminous family Lotus cornioulatus. It is an excellent perennial forage, a common garden ornamental plant, and an excellent nectar plant And soil and water conservation plants, native to the humid and warm regions of Eurasia. It is distributed in South China, Southwest my country, Northwest China, North China and other places. It is an excellent pasture grass with great development potential in temperate and subtropical regions of my country. Seeds have been used for propagation in the past, but the growth of seedlings is slow and the seed hardening rate is high. The germination rate was low due to many factors, which to some extent affected the large-scale planting of japonicu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 钟妙娥何穗华侯学瑛吴燕民卢运明张占路姜静仪濮冶民郑平
Owner SHENZHEN NONGKE GROUP
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