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Primary culture bud inducing method of lithocarpus oleaefolius

A technology of primary culture and bud induction, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of explant browning, etc., to increase the germination rate, reduce the occurrence of vitrification, and promote the induction of buds Effect

Inactive Publication Date: 2017-05-10
LIUZHOU LINGTONG TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a method that can overcome the problems of explant browning and vitrification in the bud induction process, improve the bud induction rate, bud index and bud growth status, and obtain a large number of buds with good quality, so as to meet the needs of proliferation and cultivation. Need, to promote the establishment of the tissue culture rapid propagation system of the Lavenderia tissue culture bud induction method of the first generation culture

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The hemi-lignified sprouts with a length of 6-7 cm and a diameter of 0.2-0.3 cm were selected as explants. Cut off the needles of the sprouting strips of the leaves, put them in a triangular flask, add a detergent solution with a concentration of 1 % by volume, seal the bottle mouth with gauze, and place it on a shaking table with a rotation speed of 200 r / min for 10 min. Rinse under running water for 2 h, then wash twice with sterile water, transfer the explants to the ultra-clean workbench, soak and disinfect with 0.1% mercuric chloride solution by volume for 10 min; then rinse with sterile water for 8 After ~10 times, the stem segments were cut into 3.5 cm long and inoculated into the bud induction medium, and each bottle was inoculated with 2 stem segments.

[0022] The raw material content of the bud induction medium is: 1 / 3 improved WPM medium + 6-KT 1.0 mg / L + activated carbon 10 mg / L + agar 5.0 g / L + sucrose 20.0 g / L. The ratio of components and volume to weigh...

Embodiment 2

[0025] The hemi-lignified sprouting strips with a length of 7-8 cm and a diameter of 0.2-0.3 cm were selected as explants. Cut off the needles of the sprouting strips of the leaves, put them in a triangular flask, add a 2% volume concentration of detergent solution, seal the mouth of the bottle with gauze, and place it on a shaker with a rotation speed of 200 r / min for 10 min. After that, it was rinsed under running water for 1.5 h, and then rinsed twice with sterile water. The explants were transferred to the ultra-clean workbench and immersed in 0.1% mercuric chloride solution for 10 min; then rinsed with sterile water for 8 After ~10 times, the stem segments were cut into 3.5 cm long and inoculated into the bud induction medium, and each bottle was inoculated with 2 stem segments.

[0026] The raw material content of the bud induction medium is: 1 / 3 improved WPM medium+6-KT 1.5mg / L+activated carbon 10mg / L+agar 5.0g / L+sucrose 20.0g / L. The ratio of components and volume to w...

Embodiment 3

[0029] The hemi-lignified sprouts with a length of 7-8 cm and a diameter of 0.3-0.4 cm were selected as explants. Cut off the needles of the sprouting strips of the leaves, put them in a conical flask, add a detergent solution with a concentration of 4% by volume, seal the mouth of the bottle with gauze, and place it on a shaking table with a rotational speed of 200 r / min for 15 min. Then rinsed under running water for 1 h, then rinsed twice with sterile water, transferred the explants to the ultra-clean workbench, soaked and disinfected with 0.1% mercuric chloride solution by volume for 10 min; rinsed with sterile water for 8 min. After ~10 times, the stem segments were cut into 4.0 cm long and inoculated into the bud induction medium, and each bottle was inoculated with 2 stem segments.

[0030] The raw material content of the bud induction medium is: 1 / 2 modified WPM medium+6-KT 1.5mg / L+activated carbon 10mg / L+agar 5.0g / L+sucrose 20.0g / L. The ratio of components and volume...

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Abstract

The invention discloses a primary culture bud inducing method of lithocarpus oleaefolius. The primary culture bud inducing method is characterized by being prepared by applying root coppice shoot of lithocarpus oleaefolius as an explant; after the asepsis of the explant, inoculating the explant to an inducing culture base of a high-quality primary culture bud; and culturing the explant under suitable temperature, humidity and light intensity, and inducing the bud germination. The method overcomes the problems of explant brown stain, vitrification and other problems during the bud inducing process, improves the bud inducing rate, budding rate and bud growth status, and acquires buds with large amount and high quality, and thereby meeting the demand of multiplication culture and promoting the setup of a rapid propagation system of the lithocarpus oleaefolius tissue culture.

Description

technical field [0001] The invention relates to vegetative propagation technology of Cordyceps sinensis, in particular to a method for inducing buds of primary culture of Cordyceps sinensis. Background technique [0002] Olive Leaf Ke ( Lithocarpus oleaefolius A. Camus), also known as Lithocarpus, Lithocarpus, is a tree of Fagaceae (Fagaceae), 8 to 15 meters high, with almost hairless bud scales, annual branches, petioles, leaf backs and inflorescences The rachis are easily shedding brown rust-colored or brown-yellow villous hairs, the biennial branches are dark brown-black, the lenticels are very small and difficult to detect. The bark is dark gray to gray-black, not cracked. The bark is about 13 meters high and the thickness is about 8 mm. The inner bark is light reddish brown, and the ridges are prominently prominent. The starch content of fruit is 50% to 60%. Distributed in Jiangxi, Fujian, Hunan, southern Guizhou, Guangdong, Guangxi. Born in 500 to 1200 meters abov...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 黄文卫
Owner LIUZHOU LINGTONG TECH CO LTD
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