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Method for inducing primary culture buds of Lithocarpus areca

A technology of primary culture and bud induction, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as browning of explants, improve germination rate, strengthen meristem ability, and promote bud induction. Effect

Inactive Publication Date: 2017-03-08
LIUZHOU LINGTONG TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method that can overcome the problems of explant browning and vitrification in the bud induction process, improve the bud induction rate, bud index and bud growth status, and obtain a large number of buds with good quality, thereby satisfying the requirements of proliferation and cultivation. Need, to promote the establishment of Areca palma tissue culture rapid propagation system bud induction method of the first generation culture

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Select the semi-lignified shoots of Areca palmae germinated at the base, with a length of 6-7 cm and a diameter of 0.2-0.3 cm as explants. Cut off the needles and leaves of betel nut leaves, put them in a triangular flask, add a detergent solution with a volume concentration of 1%, seal the bottle mouth with gauze, and wash it on a shaking table with a rotation speed of 200 r / min for 10 min. Rinse under running water for 2 h, then wash twice with sterile water, transfer the explants to an ultra-clean workbench and sterilize them with 0.1% mercuric chloride solution for 10 min; then rinse with sterile water for 8~ After 10 times, the stems cut to 3.5 cm long were inoculated in the bud induction medium, and 2 stems were inoculated in each bottle.

[0022] The raw material content of the bud induction medium is: 1 / 3 improved WPM medium + 6-KT 1.0 mg / L + vitamin C 10 mg / L + agar 5.0 g / L + sucrose 20.0 g / L. Wherein the improved WPM medium composition and volume to weight ra...

Embodiment 2

[0025] Select the semi-lignified shoots of Areca palmae germinated at the base, 7-8 cm in length, and 0.2-0.3 cm in diameter as explants. Cut off the needles and leaves of betel nut leaves, put them in a triangular flask, add a 2% volume concentration of detergent solution, seal the bottle mouth with gauze, and wash it for 10 minutes on a shaking table with a rotation speed of 200 r / min. Rinse under running water for 1.5 h, then wash twice with sterile water, transfer the explants to an ultra-clean workbench and sterilize them with 0.1% mercury chloride solution for 10 min; then rinse with sterile water for 8~ After 10 times, the stems cut to 3.5 cm long were inoculated in the bud induction medium, and 2 stems were inoculated in each bottle.

[0026]The raw material content of the bud induction medium is: 1 / 3 improved WPM medium + 6-KT 1.5 mg / L + vitamin C 10 mg / L + agar 5.0 g / L + sucrose 20.0 g / L. Wherein the improved WPM medium composition and volume to weight ratio are: NH...

Embodiment 3

[0029] Select the semi-lignified shoots of Areca palmae germinated at the base, with a length of 7-8 cm and a diameter of 0.3-0.4 cm as explants. Cut off the needles and leaves of betel nut leaves, put them in a triangular flask, add a 4% volume concentration of detergent solution, seal the bottle mouth with gauze, and wash it for 15 minutes on a shaker with a rotation speed of 200 r / min. Rinse under running water for 1 h, then wash twice with sterile water, transfer the explants to an ultra-clean workbench and sterilize them with 0.1% mercuric chloride solution for 10 min; then rinse with sterile water for 8~ After 10 times, the 4.0 cm long stem segments were cut and inoculated in the bud induction medium, and 2 stem segments were inoculated in each bottle.

[0030] The raw material content of the bud induction medium is: 1 / 2 improved WPM medium + 6-KT 1.5 mg / L + vitamin C 10 mg / L + agar 5.0 g / L + sucrose 20.0 g / L. Wherein the improved WPM medium composition and volume to we...

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Abstract

The invention discloses a method for inducing primary culture buds of Lithocarpus areca. The method is characterized by including: using Lithocarpus areca root coppice shoots as explants, sterilizing the explants, inoculating the explants to a high-quality primary culture bud induction culture medium, and culturing under appropriate temperature, humidity and light intensity conditions to induce germination. The method has advantages that problems such as explant browning and vitrification during bud induction are solved, bud induction rate and germination index are increased, bud growth conditions are improved, many high-quality buds can be obtained to meet the requirements of proliferation culture, and establishment of a Lithocarpus areca tissue culture and rapid propagation system is promoted.

Description

technical field [0001] The invention relates to the asexual reproduction technology of the areca palm, in particular to a method for inducing buds of the first-generation culture of the areca palm. Background technique [0002] Lithocarpus areca (Hick. et A. Camus) A. Camus is a tree of the family Fagaceae (Lithocarpus), 10-15 meters high, with off-white branchlets, glabrous, and lenticels. Leaves are papery, oblanceolate or narrowly long elliptic, 13-25 cm long, 3.5-5.5 cm wide, both ends narrowly pointed, base decelerates, a few lobed sharp teeth or entire margins on the upper part of the leaf margin, Midrib slightly raised on leaf surface, lateral veins 9 to 15 on each side, slightly sunken on leaf surface, distinct branch veins, same color on both sides, glabrous or tufted in axils of veins; petiole 0.5 to 1.5 cm long. Male spikes are axillary on a single ear, with rare short branches and conical shape, 5-8 cm long, many and dense flowers, slender filaments, rather long...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 黄文卫
Owner LIUZHOU LINGTONG TECH CO LTD
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