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Induction method for primary culture buds of quercus acutissima

A technology of primary culture and bud induction, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as browning of explants, improve germination rate, reduce pollution rate, promote growth speed and robustness Effect

Inactive Publication Date: 2017-03-08
LIUZHOU LINGTONG TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method that can overcome the problems of explant browning and vitrification in the bud induction process, improve the bud induction rate, bud index and bud growth status, and obtain a large number of buds with good quality, thereby satisfying the requirements of proliferation and cultivation. Need, to promote the establishment of the rapid propagation system of Quercus quercus tissue culture bud induction method of the first generation culture

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The semi-lignified sprouting sprouting at the base of Sawtooth oak, 6-7cm in length and 0.2-0.3 cm in diameter were selected as explants. Cut off the sprout needles of Quercus acutissima, place them in a triangular flask, add a detergent solution with a volume concentration of 1%, seal the mouth of the bottle with gauze, and place it on a shaker with a rotation speed of 200 r / min for 10 minutes. Rinse under running water for 2 hours, then rinse with sterile water twice, transfer the explants to the ultra-clean workbench and soak and disinfect with a volume concentration of 0.1% mercuric chloride solution for 10 minutes; then rinse with sterile water for 8~ After 10 times, the 3.5 cm long stems were cut and inoculated in the bud induction medium, and each bottle was inoculated with 2 stems.

[0022] The raw material content of the bud induction medium is: 1 / 3 modified WPM medium + 6-BA 1.0 mg / L + vitamin C 10 mg / L + agar 5.0 g / L + sucrose 20.0 g / L. The composition and volu...

Embodiment 2

[0025] The semi-ligninized sprouting sprouting at the base of Sawtooth oak, 7-8 cm long and 0.2-0.3 cm diameter were selected as explants. Cut off the sprout needles of Quercus acutissima, place them in a triangular flask, add a detergent solution with a volume concentration of 2%, seal the mouth of the bottle with gauze, and place it on a shaker with a rotation speed of 200 r / min for 10 minutes. Rinse under running water for 1.5 hours, then wash with sterile water twice, transfer the explants to a clean bench and soak and disinfect with a volume concentration of 0.1% mercuric chloride solution for 10 minutes; then rinse with sterile water for 8~ After 10 times, the 3.5 cm long stems were cut and inoculated in the bud induction medium, and each bottle was inoculated with 2 stems.

[0026] The raw material content of the bud induction medium is: 1 / 3 modified WPM medium+6-BA 1.5mg / L+vitamin C10mg / L+agar 5.0g / L+sucrose 20.0g / L. The composition and volume-to-weight ratio of the modi...

Embodiment 3

[0029] The semi-lignified sprouts with a length of 7-8 cm and a diameter of 0.3-0.4 cm were selected as explants. Cut off the sprout needles of Sawtooth oak, put it in a triangular flask, add a detergent solution with a volume concentration of 4%, seal the bottle with gauze, and place it on a shaker with a rotation speed of 200 r / min for 15 minutes. Rinse under running water for 1 h, then rinse with sterile water twice, transfer the explants to an ultra-clean workbench and soak and disinfect with a volume concentration of 0.1% mercuric chloride solution for 10 min; rinse with sterile water for 8~ After 10 times, the 4.0 cm long stems were cut and inoculated into the bud induction medium, and each bottle was inoculated with 2 stems.

[0030] The raw material content of the bud induction medium is: 1 / 2 modified WPM medium+6-BA 1.5mg / L+vitamin C10mg / L+agar 5.0g / L+sucrose 20.0g / L. The composition and volume-to-weight ratio of the modified WPM medium are: NH 4 NO 3 780mg / L, CaCl 2 ·...

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Abstract

The invention discloses an induction method for primary culture buds of quercus acutissima. The method is characterized by comprising the steps of adopting coppice shoots of bases of quercus acutissima as explants, subjecting the explants to sterile treatment, then, inoculating the explants to a high-quality primary-culture bud induction culture medium, and carrying out culture under the conditions of appropriate temperature, humidity and illumination intensity so as to induce the germination of the buds. According to the method, the problems such as explant browning and vitrification in a bud induction process are solved, the bud inductivity, germination index and bud growth condition are improved, and large-quantity and high-quality buds are obtained, so that the needs of propagation culture are met, and the establishment of a rapid propagation system for quercus acutissima tissue culture is promoted.

Description

Technical field [0001] The present invention relates to asexual reproduction technology of Sawtooth oak, in particular to a method for inducing buds of Sawtooth oak primary culture. Background technique [0002] Quercus acutissima Carruth (Quercus acutissima Carruth), also known as Cyclobalanopsis, Oak Bowl Tree, is a tree of the Fagaceae family Quercus. It is 30 meters tall and has a diameter of 1 meter at breast height. The bark is dark gray-brown and deeply split. Born on the sunny slopes of mountains 60-2200 meters above sea level, into small patches of pure forest or mixed forest, masculine happy light, happy humid climate. Cold-tolerant, drought-resistant and barren, intolerant to water and humidity, intolerant to saline-alkali, it grows best on humid, fertile, deep, well-drained neutral to slightly acidic sandy loam, and it is not suitable for planting on poorly drained or waterlogged soil. Mixed with other tree species can form a good stem shape, deep rooting, strong ger...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 黄文卫
Owner LIUZHOU LINGTONG TECH CO LTD
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