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Primary culture bud induction method for lithocarpus amygdalifolius

A technology of primary culture and bud induction, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of browning of explants, achieve the goal of increasing germination rate, reducing pollution rate, and reducing the occurrence of vitrification Effect

Inactive Publication Date: 2017-02-01
LIUZHOU LINGTONG TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a method that can overcome the problems of explant browning and vitrification in the bud induction process, improve the bud induction rate, bud index and bud growth status, and obtain a large number of buds with good quality, so as to meet the needs of proliferation and cultivation. Need, promote the establishment of Xingyeke tissue culture rapid propagation system of the first generation culture bud induction method of Xingyeke

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The semi-ligninized sprouts with a length of 6-7 cm and a diameter of 0.2-0.3 cm that germinated from the base of apricot leaf were selected as explants. Cut off the needles of Apricot leaf Kemeng strips, put them in a triangular flask, add a detergent solution with a volume concentration of 1%, seal the bottle with gauze, and place on a shaker with a rotation speed of 200 r / min for 10 min. Then rinsed under running water for 2 hours, then rinsed with sterile water twice, transferred the explants to the ultra-clean workbench, soaked and disinfected with a volume concentration of 0.1% mercuric chloride solution for 10 min; rinsed with sterile water for 8 After ~10 times, cut the 3.5 cm long stems and inoculate them in the bud induction medium, and inoculate 2 stems per bottle.

[0022] The raw material content of the bud induction medium is: 1 / 3 modified WPM medium + 6-BA 1.0 mg / L + activated carbon 10 mg / L + agar 5.0 g / L + sucrose 20.0 g / L. The composition and volume-to-w...

Embodiment 2

[0025] The semi-ligninized sprouts with a length of 7-8 cm and a diameter of 0.2-0.3 cm were selected as explants. Cut off the needles of Apricot Leaf Kemeng strips, place them in a triangular flask, add a detergent solution with a volume concentration of 2%, seal the mouth of the bottle with gauze, and place it on a shaker with a rotation speed of 200 r / min for 10 minutes After that, it was rinsed under running water for 1.5 hours, and then washed twice with sterile water. The explants were transferred to the ultra-clean workbench and immersed and disinfected with a volume concentration of 0.1% mercuric chloride solution for 10 minutes; then rinsed with sterile water for 8 After ~10 times, cut the 3.5 cm long stems and inoculate them in the bud induction medium, and inoculate 2 stems per bottle.

[0026] The raw material content of the bud induction medium is: 1 / 3 modified WPM medium + 6-BA 1.5 mg / L + activated carbon 10 mg / L + agar 5.0 g / L + sucrose 20.0 g / L. The composition a...

Embodiment 3

[0029] The semi-ligninized sprouts with a length of 7-8 cm and a diameter of 0.3-0.4 cm that germinated from the base of apricot leaf were selected as explants. Cut off the needles of Apricot Leaf Kemeng strips, place them in a triangular flask, add a detergent solution with a volume concentration of 4%, seal the mouth of the bottle with gauze, and place it on a shaker with a rotation speed of 200 r / min for 15 minutes Then rinsed under running water for 1 h, then rinsed with sterile water twice, transferred the explants to a clean bench and soaked and disinfected with a volume concentration of 0.1% mercuric chloride solution for 10 min; rinsed with sterile water for 8 After ~10 times, cut the 4.0 cm-long stem segments and inoculate them in the bud induction medium, and inoculate 2 stem segments per bottle.

[0030] The raw material content of the bud induction medium is: 1 / 2 modified WPM medium + 6-BA 1.5 mg / L + activated carbon 10 mg / L + agar 5.0 g / L + sucrose 20.0 g / L. The com...

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Abstract

The invention discloses a primary culture bud induction method for lithocarpus amygdalifolius. The method is characterized by comprising the following steps: taking a base coppice shoot of the lithocarpus amygdalifolius as an explant, performing aseptic treatment on the explant, inoculating the explant to a high-quality primary culture bud induction medium, culturing under the conditions of appropriate temperature, humidity and illumination intensity, and inducing bud germination. According to the method disclosed by the invention, the problems such as explant browning, vitrification and the like in the bud induction process are solved, the bud induction rate, budding index and germination and growth conditions are improved, and a great number of high-quality buds are obtained, so that the multiplication culture requirements are met, and establishment of a rapid tissue culture propagation system for the lithocarpus amygdalifolius is facilitated.

Description

Technical field [0001] The present invention relates to asexual propagation technology of Apricot Yew, in particular to a method for inducing buds of the first generation culture of Apricot Yew. Background technique [0002] Lithocarpus amygdalifolius (Skan) Hayata, also known as Lithocarpus amygdalifolius, Red Asparagus, and Lithocarpus, is a tree of the Fagaceae family (Lithocarpus). The new branches and young leaves are densely covered in spring and summer. Brown curly pilose, all hairs fall off after autumn. The male spikes are axillary or multiple spikes arranged in panicles, the inflorescences are densely pilose; the female flowers are in clusters of 3, sometimes with single scattered. The shell is nearly spherical, full of nuts, in a discontinuous ring shape; the fruit wall of the nut is slightly thicker than the shell, and the top of the nut is puberulent. Distributed in central and southern Taiwan, southern Fujian, areas south of the Tropic of Cancer in southeastern Gu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001
Inventor 黄文卫
Owner LIUZHOU LINGTONG TECH CO LTD
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