Inducing method for primary culture buds of lithocarpus hancei

A technology of primary culture and bud induction, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of browning of explants, achieve the effects of increasing germination rate, reducing pollution rate, and overcoming vitrification

Inactive Publication Date: 2017-02-01
LIUZHOU LINGTONG TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method that can overcome the problems of explant browning and vitrification in the bud induction process, improve the bud induction rate, bud index and bud growth status, and obtain a large num

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The semi-lignified sprouts with a length of 6-7 cm and a diameter of 0.2-0.3 cm that germinated from the base of the hard-shelled Cory spp. were selected as explants. Cut off the needles and leaves of the hard shell Kemengtiao, put them in a triangular flask, add a solution of detergent with a volume concentration of 1%, seal the bottle mouth with gauze, and wash it on a shaking table with a rotation speed of 200 r / min for 10 min After that, rinse them under running water for 2 h, then wash them twice with sterile water, transfer the explants to an ultra-clean workbench and sterilize them with 0.1% mercury chloride solution for 10 min; then rinse them with sterile water for 8 min. After ~10 times, cut the 3.5 cm long stem segments and inoculate them in the bud induction medium, and inoculate 2 stem segments in each bottle.

[0022] The raw material content of the bud induction medium is: 1 / 3 improved WPM medium + TDZ 1.0 mg / L + vitamin C 10 mg / L + lactose 5.0 g / L + sucr...

Embodiment 2

[0025] The semi-lignified sprouts with a length of 7-8 cm and a diameter of 0.2-0.3 cm were selected as explants. Cut off the needles and leaves of the hard shell Kemengtiao, put them in a triangular flask, add a solution of detergent solution with a volume concentration of 2%, seal the mouth of the bottle with gauze, and wash it on a shaker at a speed of 200 r / min for 10 minutes After that, rinse under running water for 1.5 h, then wash twice with sterile water, transfer the explants to an ultra-clean workbench and sterilize them with 0.1% mercury chloride solution for 10 min; then rinse with sterile water for 8 After ~10 times, cut the 3.5 cm long stem segments and inoculate them in the bud induction medium, and inoculate 2 stem segments in each bottle.

[0026] The raw material content of the bud induction medium is: 1 / 3 improved WPM medium + TDZ 1.5 mg / L + vitamin C 10 mg / L + lactose 5.0 g / L + sucrose 20.0 g / L. Wherein the improved WPM medium composition and volume to wei...

Embodiment 3

[0029] The semi-lignified sprouts with a length of 7-8 cm and a diameter of 0.3-0.4 cm, which germinated from the base of the hard-shelled Cory spp., were selected as explants. Cut off the needles and leaves of the hard shell Kemengtiao, put them in a triangular flask, add a solution of detergent solution with a volume concentration of 4%, seal the bottle mouth with gauze, and wash it on a shaker at a speed of 200 r / min for 15 minutes After that, rinse under running water for 1 h, then wash twice with sterile water, transfer the explants to an ultra-clean workbench and sterilize them with 0.1% mercury chloride solution for 10 min; then rinse with sterile water for 8 After ~10 times, the 4.0 cm long stem segments were inoculated in the bud induction medium, and 2 stem segments were inoculated in each bottle.

[0030] The raw material content of the bud induction medium is: 1 / 2 improved WPM medium + TDZ 1.5 mg / L + vitamin C 10 mg / L + lactose 5.0 g / L + sucrose 20.0 g / L. Wherein ...

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Abstract

The invention discloses an inducing method for primary culture buds of lithocarpus hancei. The inducing method is characterized by comprising the steps that base coppice shoots of the lithocarpus hancei are adopted as explants; after the explants are subjected to aseptic processing, a high-quality primary culture bud inducing medium is inoculated with the explants; the explants are placed under the appropriate temperature, humidity and illumination intensity conditions to be cultured, and bud germination is induced. According to the inducing method, the problems of browning, vitrifaction and the like of the explants in the bud inducing process are solved, the bud inducing rate and the budding index are increased, the germinated bud growth state is improved, more high-quality germinated buds are obtained, therefore, the multiplication culture requirement is met, and establishment of a rapid propagation system for lithocarpus hancei tissue culture is promoted.

Description

technical field [0001] The invention relates to the asexual reproduction technology of Fructus fragrans, in particular to a method for inducing buds cultured in the first generation of Fructus fragrans. Background technique [0002] hard shell Ke ( Lithocarpus hancei (Benth.) Rehd ) also known as Chipigang (Taiwan, China), Jiuyan (Jiangxi), Bangzhao (Hunan), Shishanzhao (Guangxi), belonging to Fagaceae (Fagaceae) Lithocarpus (Lithocarpus), tree, rarely more than 15 meters high , all parts glabrous except inflorescence rachis and cupule gray pubescent. Branchlets yellowish gray or gray, often with a thin transparent waxy layer. Leaves are papery to hard leathery, ovate, obovate, broadly elliptic, obovate-elliptic, narrowly oblong-elliptic or lanceolate. The flowering period is from April to June, and the fruit matures from September to December of the following year. Bark dark gray or brownish gray, 6-10 mm thick, irregularly lobed longitudinally, peeled in small flakes, ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001
Inventor 黄文卫
Owner LIUZHOU LINGTONG TECH CO LTD
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