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Asexual propagation seedling method of hybrid paper mulberry

A technology for rapid propagation of seedlings and tree building, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of low rooting rate and low survival rate of culture materials, and achieve improved environmental adaptability, high seedling efficiency, The effect of improving efficiency

Inactive Publication Date: 2018-07-31
天长市金农农业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the existing hybrid mulberry tissue culture and rapid propagation technology, the culture materials have problems such as low rooting rate and low survival rate.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A kind of tissue culture fast breeding seedling method of hybrid tree that the present invention proposes, comprises the following steps:

[0022] S1, explant selection: get the new spruce that the hybrid tree takes out in the same year, cut the branch into 2-3cm bud stem segments as explants, clean them with washing powder aqueous solution, rinse, and then carry out disinfection and sterilization treatment;

[0023] S2. Induction culture: inoculate the explants on the induction medium, and use LED lights to cultivate and induce adventitious buds; the composition of the induction medium is as follows: MS+0.7mg / L BA+0.1mg / L NAA+30.0 g / L sucrose+5.5g / L agar, adjust the pH to 5.8-6.0;

[0024] S3. Subculture: inoculate the induced adventitious buds on the proliferation medium, and use LED light to illuminate for subculture; the composition of the proliferation medium is as follows: WPM+1.0mg / L BA+0.5mg / LKT +30.0g / L sucrose+5.5g / L agar, adjust the pH to 5.8-6.0;

[0025] ...

Embodiment 2

[0028] A kind of tissue culture fast breeding seedling method of hybrid tree that the present invention proposes, comprises the following steps:

[0029] S1. Selection of explants: Take the new shoots of the hybrid tree in the same year, cut the unlignified branches into 2-3cm budding stems as explants, clean them with washing powder aqueous solution, rinse for 10 minutes, and put Soak the explants in 70% alcohol for 20s, disinfect with 0.1% HgCl2 solution on a clean bench for 5min, and rinse with sterile water for 3-5 times;

[0030] S2. Induction culture: inoculate the explants on the induction medium, and use LED light to illuminate and cultivate the induced adventitious buds. The light culture is 30 days, and the light cycle is 13h / d; the composition of the induction medium is as follows: MS+0.7mg / L BA+0.1mg / LNAA+30.0g / L sucrose+5.5g / L agar, adjust the pH to 5.8-6.0;

[0031] S3, subculture: Inoculate the induced adventitious buds on the proliferation medium, and use LED...

Embodiment 3

[0035] A kind of tissue culture fast breeding seedling method of hybrid tree that the present invention proposes, comprises the following steps:

[0036] S1, explant selection: take the new shoots of the hybrid tree that were taken in the same year, cut the unlignified branches into 2-3cm bud stem segments as explants, clean them with washing powder aqueous solution, rinse for 15 minutes, and put Soak the explants in 70% alcohol for 25s, disinfect with 0.1% HgCl2 solution on a clean bench for 8min, and rinse with sterile water for 3-5 times;

[0037] S2. Induction culture: inoculate the explants on the induction medium, use LED lights to illuminate and cultivate to induce adventitious buds, light culture for 33 days, and the light cycle is 14h / d; the composition of the induction medium is as follows: MS+0.7mg / L BA+0.1mg / LNAA+30.0g / L sucrose+5.5g / L agar, adjust the pH to 5.8-6.0;

[0038] S3, subculture: Inoculate the induced adventitious buds on the proliferation medium, and...

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Abstract

The invention discloses a tissue culture rapid propagation seedling method of hybrid paper mulberry, and belongs to the technical field of hybrid paper mulberry plantation. The tissue culture rapid propagation seedling method of hybrid paper mulberry comprises following steps: explant selection, induced culturing, subculturing, rooting culture, and transplanting. According to the tissue culture rapid propagation seedling method, current year stems with buds are selected as explants, the meristematic capacity is excellent, and differentiation propagation is more convenient to realize; LED illumination culture is capable of adjusting culture material growth process, increasing the environmental adaptability, realizing reasonable optimization on composition of induction medium, proliferationmedium, and rooting medium, and illumination conditions of different stages, increasing seedling tissue culture efficiency, and achieving relatively induction rate, reproduction rate, and rooting rate; the adventitious bud induction differentiation rate is 94% or higher; after subculture, average seedling height reaches 3.5cm, rooting rate reaches 100%, and transplanting survival rate reaches 68%or higher. The tissue culture rapid propagation seedling method is adopted for seedling of hybrid paper mulberry, the seedling efficiency is high, the propagation coefficient is large, seedling cost is reduced, and the obtained tissue culture seedlings are strong, and are high in quality.

Description

technical field [0001] The invention relates to the technical field of hybrid tree planting, in particular to a method for asexually propagating seedlings of hybrid tree. Background technique [0002] The hybrid mulberry is a plant of the genus Moraceae. It is an aerospace hybrid mulberry that has been cultivated by comprehensive methods such as space-borne space breeding in my country in the past ten years. It has the characteristics of fast growth, high yield, high quality, and multi-resistance. The leaves of the mulberry tree contain crude protein and flavonoids. It is a high-quality plant protein feed. The comprehensive nutritional content exceeds that of bean plants. It can replace soybean meal and fish meal for the breeding of various animals. [0003] The seedling raising methods of hybrid mulberry tree include seedling cutting seedling raising and tissue culture rapid propagation seedling raising. However, the propagation coefficient of cutting seedlings is low, the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 孙巨福戴宇钧任永国
Owner 天长市金农农业发展有限公司
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